Gonadal response of juvenile protogynous grouper (Epinephelus fuscoguttatus) to long term recombinant follicle-stimulating hormone administration
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The role of follicle stimulating hormone (FSH) in the gonadal development of protogynous hermaphroditic grouper (E. fuscoguttatus) was investigated. Recombinant giant grouper (E. lanceolatus) FSH (rggFSH) was produced in yeast. Its receptor binding capacity and steroidogenic potency were confirmed in vitro. Weekly injections of rggFSH to juvenile tiger grouper for 8 weeks (100 μg/kg body weight, BW) resulted in significantly larger and more advanced oocytes (cortical alveolar stage vs. primary growth stage in control). Sustained treatment with rggFSH (20 to 38 weeks at 200 μg/kg BW) resulted in significant reduction in gonad size, degeneration of oocytes and proliferation of spermatogonial cells, indicative of female to male sex change. Gene expression analysis showed that, while initiating female to male sex change, the rggFSH significantly suppressed the steroidogenic genes cyp11b, cyp19a1a and foxl2 which restrained the endogenous production of sex steroid hormones thus prevented the differentiation of spermatogonial cells. Expression profile of sex markers dmrt1, amh, figla and bmp15 suggests that the observed sex change was restricted at the initiation stage. Based on these results, we propose that the process of female to male sex change in the protogynous grouper is initiated by FSH, rather than sex steroids and likely involves steroid-independent pathway. The cortical alveolar stage in oocyte development is the critical point after which FSH-induced sex change is possible in grouper.
Palma, P., Nocillado, J., Superio, J., Ayson, E. G. de J., Ayson, F., Bar, I., & Elizur, A. (2018). Gonadal response of juvenile protogynous grouper (Epinephelus fuscoguttatus) to long term recombinant follicle-stimulating hormone administration. Biology of Reproduction. https://doi.org/10.1093/biolre/ioy228
PublisherOxford University Press
This study was funded by the Australian Centre for International Agricultural Research (ACIAR) through project no. FIS/2012/101 granted to A. Elizur. P. Palma was a recipient of an ACIAR funded Australia Awards John Allwright Fellowship.
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Conference paperAC Emata - In TU Bagarinao & EEC Flores (Eds.), Towards sustainable aquaculture in Southeast Asia and Japan: Proceedings of the Seminar-Workshop on Aquaculture Development in Southeast Asia, Iloilo City, Philippines, 26-28 July, 1994, 1995 - Aquaculture Department, Southeast Asian Fisheries Development CenterMost of the fish research at SEAFDEC AQD in 1992-1994 was on milkfish. Studies were conducted on year-round spawning through hormonal or environmental manipulation; optimum lipid and protein levels and ration size for captive broodstock; and the influence of spawner age on reproductive performance. The economics of hatchery operations, alone or integrated with broodstock as a commercial enterprise, was assessed. Mass production of larvae was refined with the use of commercial or SEAFDEC-formulated larval diets. Alternative rearing schemes in large tanks and ponds were tried. Hatcheryproduced and wild-caught larvae were compared in terms of growth and production in experimental nursery and grow-out ponds. Supplemental diets for brackishwater grow-out culture were formulated. Studies on broodstock management of grouper Epinephelus spp. included lipid enrichment of the diet and hormonal induction of sex inversion. Seed production techniques were developed but survival rates were low. Grouper culture was found economically feasible in experimental ponds with 'trash' fish as feed. The mangrove red snapper Lutjanus argentimaculatus was successfully induced to spawn with injection of human chorionic gonadotropin. Initial larval rearing trials were successful but survival rates must be improved. Hormonal manipulation of spawning of the Asian sea bass Lates calcarifer allows seed production during most of the year. Photoperiod manipulation leads to maturation of females, but not males, beyond the natural breeding season (April-November). Nursery rearing of 9 mm juveniles is feasible in floating net cages with night lights that attract food zooplankton. The requirements of sea bass for lipid, protein, carbohydrates, and essential amino acids were determined. In the rabbitfish Siganus guttatus, weekly injections of luteinizing hormone releasing hormone analogue (LHRHa) sustains milt production for three weeks. Thyroid hormones injected into broodstocks improved the growth of larvae to day 7. Induced spawning techniques for the Asian catfish Clarias macrocephalus were refined by determining the seasonal responsiveness to LHRHa and pimozide injections and testing for pheromonal induction of spontaneous spawning. The optimum insemination rate was determined and egg hatchability was enhanced by removal of the adhesive coat before incubation. Several practical diets for catfish during grow-out culture were tested against 'trash' fish. The broodstock management for bighead carp Aristichthys nobilis was studied. Cage-reared juveniles from cage-reared broodstock showed the best growth. To improve the reproductive performance, the broodstock diets were supplemented with vitamins A, C, and E. Research on tilapias focused on genetics and strain selection. Several strain testing procedures for Nile tilapia were evaluated in their efficiency to detect economically important strain differences. Reference lines were developed from two existing red tilapia strains to measure and reduce the effects of uncontrolled nongenetic variables in strain evaluation experiments with Nile tilapia. The tolerance of two Nile tilapia strains to heavy metals was similar when gauged by the 24-hour and 96-hour lethal concentration and by fish growth, survival, and reproductive performance. In a separate study, four strains of red tilapia showed generally higher seed production when reared in tanks than in cages. Improvements in the feed and feeding management for Nile tilapia were also studied. Intensive tilapia farming and feeding have led to oxygen depletion and fish kills in Sampaloc Lake. To rehabilitate the lake, it is imperative to reduce the farming area from 30 to 6 hectares; stop the use of commercial feeds; and remove the water hyacinths and other debris. Fish kills in Laguna de Bay have also become serious in recent years, and a review of the occurrences, losses, and possible causes is currently being conducted. Studies on the epizootic ulcerative syndrome of snakeheads in Laguna de Bay have yet to pinpoint the pathogen. Skin lesions in tilapias in several ponds and lakes in the country were found to be due to bacteria.
Conference paperHM Ali - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development CenterResearch on grouper (Epinephelus suillus) fry production in captivity has been carried out in Malaysia since 1986 at Tanjung Demong Marine Finfish Production and Research Centre (TDMFPRC) but the breakthrough was only achieved four years later in 1990. Eggs were obtained through natural and induced spawning in tanks. Natural spawning of grouper in captivity seldom occurred and was unpredictable. However induced spawnings were successfully carried out by injecting human chorionic gonadotropin (hCG) intramuscularly at a dose of 500-1000 IU/kg fish. The results from several trials on larval rearing conducted since 1989 until recently showed that larvae obtained from natural spawnings survived longer with some reaching the juvenile stage. The highest recorded survival rate of 43 days posthatch (32.5 mm total length) was 12.1% at 28-32 °C water temperature. On the other hand, 100% mortality usually occurred in larvae obtained from induced spawning 7 days after hatching. The major constraints of grouper fry production in Malaysia are lack of male spawners, inconsistent and unpredictable natural spawning, small quantity of eggs released every spawning day, poor fertilization and hatching rate, weak hatchlings, and high mortality rate at the early stages of larval development. The latter is probably due mainly to problems on initial feeding.
Conference paperGF Quinitio, RM Coloso, A Duller & D Reyes - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development CenterThe effect of different fat sources on the egg quality of grouper, Epinephelus suillus eggs was evaluated. Fish in three tanks, each containing 3 females and 1 male, were fed various types of feeds namely: trash fish (control), trash fish + cod liver oil (treatment 1) and trash fish + SELCO, a lipid emulsion containing high levels of highly unsaturated fatty acid (HUFA) (treatment 2). Approximately 77.9 million eggs spawned from January to October 1992 by the control group, 40.0 million by fish in treatment 1, and 36.4 million by the treatment 2 group. Egg production (0.45 million eggs/kg BW) among the control group was significantly higher than treatment 2. Egg production of treatment 1 (0.06-0.36 million eggs/kg BW) was not significantly different (P<0.01) from the control group nor treatment 2 (0.02-0.30 million eggs/kg BW). Fertilization and hatching rates showed significant differences among the three groups with control > treatment 1 > treatment 2. There were no differences detected in the egg and oil globule diameters among the treatments. Crude protein and lipid levels of floating (good) and sinking (bad) eggs collected in February to March 1992, and August to September 1992 were similar in all treatments. Unfed larvae from treatment 1 survived until the fifth day after hatching while those in the control and treatment 2 groups lasted only until the third day. These results suggest that supplementation of cod liver oil and SELCO in the trash fish diet of E. suillus broodstock does not influence egg production, fertilization and hatching rates, and egg quality.