SEAFDEC/AQDINSTITUTIONAL REPOSITORY
    • English
    • 日本語
    • ไทย
    • Bahasa Indonesia
  • 日本語 
    • English
    • 日本語
    • ไทย
    • Bahasa Indonesia
  • ログイン
アイテム表示 
  •   ホーム
  • 03 SEAFDEC/AQD External Publications
  • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
  • Journal Articles
  • アイテム表示
  •   ホーム
  • 03 SEAFDEC/AQD External Publications
  • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
  • Journal Articles
  • アイテム表示
JavaScript is disabled for your browser. Some features of this site may not work without it.

Gonadal response of juvenile protogynous grouper (Epinephelus fuscoguttatus) to long term recombinant follicle-stimulating hormone administration

  • Global styles
  • MLA
  • Vancouver
  • Elsevier - Harvard
  • APA
  • Help
Thumbnail
閲覧/開く
Request a copy
日付
2019-03
著者
Palma, Peter ORCID
Nocillado, Josephine
Superio, Joshua
Ayson, Evelyn Grace de Jesus
Ayson, Felix
Bar, Ido
Elizur, Abigail
Page views
3,425
主題
sex reversal ASFA
sex hormones ASFA
genetic processes ASFA
protogyny ASFA
groupers AGROVOC
Follicle-stimulating hormone
Recombinant gonadotropins
Protogynous
Epinephelus lanceolatus AGROVOC
Taxonomic term
Epinephelus fuscoguttatus GBIF
Metadata
アイテムの詳細レコードを表示する

Cited times in Scopus



Share 
 
抄録
The role of follicle stimulating hormone (FSH) in the gonadal development of protogynous hermaphroditic grouper (E. fuscoguttatus) was investigated. Recombinant giant grouper (E. lanceolatus) FSH (rggFSH) was produced in yeast. Its receptor binding capacity and steroidogenic potency were confirmed in vitro. Weekly injections of rggFSH to juvenile tiger grouper for 8 weeks (100 μg/kg body weight, BW) resulted in significantly larger and more advanced oocytes (cortical alveolar stage vs. primary growth stage in control). Sustained treatment with rggFSH (20 to 38 weeks at 200 μg/kg BW) resulted in significant reduction in gonad size, degeneration of oocytes and proliferation of spermatogonial cells, indicative of female to male sex change. Gene expression analysis showed that, while initiating female to male sex change, the rggFSH significantly suppressed the steroidogenic genes cyp11b, cyp19a1a and foxl2 which restrained the endogenous production of sex steroid hormones thus prevented the differentiation of spermatogonial cells. Expression profile of sex markers dmrt1, amh, figla and bmp15 suggests that the observed sex change was restricted at the initiation stage. Based on these results, we propose that the process of female to male sex change in the protogynous grouper is initiated by FSH, rather than sex steroids and likely involves steroid-independent pathway. The cortical alveolar stage in oocyte development is the critical point after which FSH-induced sex change is possible in grouper.
URI
http://hdl.handle.net/10862/3354
Suggested Citation
Palma, P., Nocillado, J., Superio, J., Ayson, E. G. d. J., Ayson, F., Bar, I., & Elizur, A. (2019). Gonadal response of juvenile protogynous grouper (Epinephelus fuscoguttatus) to long term recombinant follicle-stimulating hormone administration. Biology of Reproduction, 100(3), 798-809. https://doi.org/10.1093/biolre/ioy228 
DOI
10.1093/biolre/ioy228
Type
Article
ISSN
0006-3363; 1529-7268
Collections
  • Journal Articles [1163]

Related items

Showing items related by title, author, creator and subject.

  • Thumbnail

    Research on marine and freshwater fishes 

    Emata, Arnil C. (Aquaculture Department, Southeast Asian Fisheries Development Center, 1995)
    Most of the fish research at SEAFDEC AQD in 1992-1994 was on milkfish. Studies were conducted on year-round spawning through hormonal or environmental manipulation; optimum lipid and protein levels and ration size for ...
  • Thumbnail

    Transport of hatchery-reared and wild grouper larvae, Epinephelus sp. 

    Estudillo, Chona B.; Duray, Marietta N. (Elsevier, 2003)
    Optimum packing conditions for the transport of hatchery-reared and wild grouper larvae were investigated under simulated condition or actual air transport. Simulation of transport motion was done through the use of an ...
  • Thumbnail

    Broodstock management and seed production of marine fishes 

    Paniza, J. R. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2001)

© SEAFDEC/AQD  2022
連絡先 | ご意見をお寄せ下さい
 

 

ブラウズ

リポジトリ全体コミュニティ/コレクション公開日著者タイトル主題このコレクション公開日著者タイトル主題

登録利用者

ログイン登録

統計

View Usage Statistics

© SEAFDEC/AQD  2022
連絡先 | ご意見をお寄せ下さい
 

 

Export citations

Export the current results of the search query as a citation list. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

The list of citations that can be exported is limited to items.

Export citations

Export the current item as a citation. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

Export Citations

DOCUMENT REQUEST NOT AVAILABLE

This publication is still available (in PRINT) and for sale at AQD bookstore. The library is currently restricted to send PDF of publications that are still for sale.

You may contact bookstore@seafdec.org.ph or visit AQD bookstore for orders.

FILE UNDER EMBARGO

This file associated with this publication is currently under embargo. This will be available for download after the embargo date.