Serum steroid hormones, reproductive and larval performance of the Asian catfish Clarias macrocephalus (Gunther) during the off- and peak reproductive seasons
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Serum samples were taken from gravid female catfish 0 to 24 h after injection of luteinizing hormone releasing hormone analogue (LHRHa) and pimozide (PIM) to monitor the serum steroid hormone levels during the off seasons (February) and the peak of the natural breeding period (August). A parallel study was also conducted to compare egg production, fertilization, hachuring and larval rates upon yolk resorption from spawn’s of hormone-treated fish. Serum E2 levels were significantly different only with varying times after injection (PI); a marked increase occurred at 13 h, but the elevation was higher in fish induced to ovulate during the peak (16.8 ng/ml) than off season(7.7ng/ml). Hormone-treated fish showed higher serum testosterone (T) levels during the peak season (17-23 ng/ml) than those injected during the off season (10-20 ng/ml) at 4-12 h PI. Serum 17¤, 20ß-dihydroxy-4-pregnene-3-one (DHP) levels og hormone-treated fish during the off season were only about half the level (0.29 and 0.52ng/ml) of those treated with the same hormones during the peak season (0.54 and 0.9 ng/ml) at 8 and 12 h PI, respectively. Initial eggs size was larger in fish induced to spawn in August (1.54 mm) than in fish induced in February (1.49 mm). All fish ovulated when induced to spawn during the peak of the natural breeding period, but ovulation rate was only 60% when were injected during the off season. Egg production, fertilization, hatchery and survival rates were similarly much higher when gravid females were induced to spawn in August (88 eggs/g body weight, 97%, 73%, and 95%, respectively) than those spawned in February (20 eggs/g BW,36%, 20%, and 47%, respectively). Results indicate that more eggs and of better quality, higher levels of T and DHP were produced during the peak season.
Tan-Fermin, J. D., Marte, C. L., Adachi, S., & Yamauchi, K. (2001). Serum steroid hormones, reproductive and larval performance of the Asian catfish Clarias macrocephalus (Gunther) during the off- and peak reproductive seasons. In J. Y.-L. Yu (Ed.), Recent Advances in Comparative Endocrinology. Proceedings of the Fourth Congress of the Asia and Oceana Society for Comparative Endocrinology, May 14-18, 2000, Academia Sinica, Taipei, Taiwan, R.O.C. (pp. 288–297). Taipei, Taiwan, R.O.C.: Institute of Zoology, Academia Sinica.
PublisherInstitute of Zoology, Academia Sinica, Taipei, Taiwan, R.O.C.
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Conference paperAD Munro & TJ Lam - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development CenterDespite their great variety of reproductive strategies, a general characteristic of most teleosts is that (where known) natural reproduction shows a long-term periodicity, even in tropical habitats (e.g. see contributions in Munro et al. 1990a). Typically, gonad development from puberty leads to fully-grown gonads by the advent of the spawning season; if conditions are appropriate, then final gonad maturation leads to the production of viable gametes during one or more breeding bouts. Subsequently, in those larger species which spawn over more than one season, the gonads regress and the individual returns to a phase where any growth is somatic. However, there is wide interspecific variability in the pattern of gonad development (Wallace and Selman 1981, de Vlaming 1983, Billard 1986, Selman and Wallace 1989).
Conference paperWE Vanstone, AC Villaluz & LB Tiro Jr. - In Proceedings of the International Milkfish Workshop Conference, May 19-22, 1976, Tigbauan, Iloilo, Philippines, 1976 - Aquaculture Department, Southeast Asian Fisheries Development CenterNewly captured milkfish released 0.8 mm in diameter non-hydrated eggs spontaneously in captivity. After injection with partially purified salmon gonadotropin (SG-G100), 1.2 mm in diameter hydrated eggs were released. These eggs, however, were not fertilized.
Effect of juvenile hormone and serotonin (5-HT) on mixis induction of the rotifer Brachionus plicatilis Muller WG Gallardo, A Hagiwara & TW Snell -
Journal of Experimental Marine Biology and Ecology, 2000 - ElsevierJuvenile hormone (JH) and serotonin (5-HT) were previously shown to enhance mictic (sexual) female production of the rotifer Brachionus plicatilis in batch cultures. To explore the basis of these effects, experiments were conducted on isolated individuals. JH treatment of maternal rotifers with 5 and 50 µg ml-1 (18.8 and 187.7 µM) resulted in significantly higher (P < 0.05) mictic female production in the second (F2) and third (F3) generations. JH treatment was effective even at a lower food concentration of 7 × 105 cells ml , but it was not effective when free ammonia was added at 2.4 and 3.1 µg ml-1. Mictic female production was not increased with exposure to 5-HT up to 50 µg ml-1 (129.1 µM) concentrations. When food level was reduced to 7 × 105 cells ml-1, however, 5-HT-treated rotifers produced significantly (P < 0.05) more mictic females than the control, particularly in F3 generation. Mictic female production of 5-HT-treated rotifers did not differ from that of the control with or without free ammonia, but the intrinsic rate of natural increase (r) of 5-HT-treated rotifers at 3.1 µg ml-1 free ammonia was significantly higher than the control. These results show that juvenile hormone increases mictic female production under optimum and sub-optimum food levels, whereas 5-HT increases both mictic female production at low food level and population growth rate at high free ammonia concentrations. These compounds could be used to manage rotifer cultures and probe the mechanisms controlling the rotifer life cycle as it switches to mictic reproduction.