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LHRHa and pimozide-induced breeding in the catfish, Clarias macrocephalus (Gunther).

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Date
1994
Author
Tan, A.M.
Garcia, M.A.
Tan-Fermin, Josefa D.
Gapasin, Rolando S.J..
Page views
2,438
Subject
food fish ASFA
catfish culture ASFA
induced ovulation ASFA
hormones ASFA
sexual maturity ASFA
spawning ASFA
eggs AGROVOC
Egg quality
Philippines AGROVOC
Catfishes
Clarias macrocephalus AGROVOC
Metadata
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Abstract
Clarias macrocephalus is endemaic yet dwinding freshwater foodfish in the Philippines. Induced breeding protocol was developed after a series of studies which determined the appropriate egg size, proper time of stripping females, effective dose combination of luteinizing hormone-releasing hormone analogue (LHRHa) and pimozide (PIM), and their effects on egg quality. Based on its similar osmotic pressure with catfish plasma, eggs were fixed in 1% phosphate-bufferedd formalin. Catfish eggs with a mean diameter of 1.40 - 1.60 mm were responsive to hormonal treatment and increased to 1.50 - 1.70 mm at ovulation. Oocyte maturation, indicated by eggs in the germinal vesicle breadown (GVBD) stage, was observed at least 13 h pst-injection, followed by ovulation 4 h thereafter. Techniques developed in these studies showed a simsle, convenient, and effective way to induce spawning of C. macrocephalus. A simultaneous injetion of 0.05 ųg LHRHa + 1 ųg PIM/g BW at 1800 - 1900 h, followed by stripping at 16 - 20 h post-injection has consistently resulted in 100% ovulation, high fertilization and hatching rates.
URI
http://hdl.handle.net/10862/392
Type
Conference paper
Koleksi
  • Conference Proceedings [288]

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