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    LHRHa and pimozide-induced breeding in the catfish, Clarias macrocephalus (Gunther).

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    Date
    1994
    Author
    Tan, A.M.
    Garcia, M.A.
    Tan-Fermin, Josefa D.
    Gapasin, Rolando S.J..
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    Abstract
    Clarias macrocephalus is endemaic yet dwinding freshwater foodfish in the Philippines. Induced breeding protocol was developed after a series of studies which determined the appropriate egg size, proper time of stripping females, effective dose combination of luteinizing hormone-releasing hormone analogue (LHRHa) and pimozide (PIM), and their effects on egg quality. Based on its similar osmotic pressure with catfish plasma, eggs were fixed in 1% phosphate-bufferedd formalin. Catfish eggs with a mean diameter of 1.40 - 1.60 mm were responsive to hormonal treatment and increased to 1.50 - 1.70 mm at ovulation. Oocyte maturation, indicated by eggs in the germinal vesicle breadown (GVBD) stage, was observed at least 13 h pst-injection, followed by ovulation 4 h thereafter. Techniques developed in these studies showed a simsle, convenient, and effective way to induce spawning of C. macrocephalus. A simultaneous injetion of 0.05 ųg LHRHa + 1 ųg PIM/g BW at 1800 - 1900 h, followed by stripping at 16 - 20 h post-injection has consistently resulted in 100% ovulation, high fertilization and hatching rates.
    URI
    http://hdl.handle.net/10862/392
    Type
    Conference paper
    Subject
    Finfishes; Catfish culture; Induced breeding; Hormones; Sexual maturity; Eggs; Egg quality; Spawning; Philippines; Catfishes; Clarias macrocephalus
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    • Conference Proceedings [281]


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