Selection of probiotics for shrimp and crab hatcheries.
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A study aimed at obtaining a biological control agent against bacterial diseases, specifically luminescent vibriosis, of hatchery-reared shrimps and crabs was done to find an alternative for chemotherapy as a disease prevention and control method. Bacteria were isolated from crustacean rearing environments where luminescent vibrosis was not observed, from natural food, and from various feed ingredients. From hundreds of purified strains, 80 bacterial isolates were tested in one-on-one mixed cultures in seawater for their ability to suppress the growth of luminescent Vibrio harveyi. Of the 10 isolates exhibiting that capability, two strains were further studied: C1 from chlorella culture and P9 from a commercial probiotic preparation. However, due to the indigenous nature of C1 strain from the unicellular alga Chlorella sp. and the ease in distinguishing it from other bacteria owing to its colony morphology, more tests were done on C1 strain. To determine the suitability of C1, and to some extent P9, as biocontrol bacteria, their pathogenicity against crab larvae and shrimp postlarvae, and their ability to become associated or incorporated into the larvae were determined. Incorporation into the rotifer, Brachionus, was also tested. Due to the positive results obtained in the incorporation experiments, the growth of strain C1 in microbiological media and unrefined media prepared from agricultural by-products was also tested.
Lavilla-Pitogo, C. R., Catedral, D. D., Pedrajas, S. A. G., & De la Peña, L. D. (2002). Selection of probiotics for shrimp and crab hatcheries. In Y. Inui & E. R. Cruz-Lacierda (Eds.), Disease Control in Fish and Shrimp Aquaculture in Southeast Asia – Diagnosis and Husbandry Techniques: Proceedings of the SEAFDEC-OIE Seminar-Workshop on Disease Control in Fish and Shrimp Aquaculture in Southeast Asia – Diagnosis and Husbandry Techniques, 4-6 December 2001, Iloilo City, Philippines (pp. 136–150). Tigbauan, Iloilo, Philippines: SEAFDEC Aquaculture Department.
PublisherAquaculture Department, Southeast Asian Fisheries Development Center
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Conference paperM Bonifacio - In ET Quinitio, FD Parado-Estepa & RM Coloso (Eds.), Philippines : In the forefront of the mud crab industry development : proceedings of the 1st National Mud Crab Congress, 16-18 November 2015, Iloilo City, Philippines, 2017 - Aquaculture Department, Southeast Asian Fisheries Development CenterThe polyculture of mud crab with one to three other species (milkfish (Chanos chanos), tilapia (Oreochromis spp.) and shrimp or sugpo (Penaeus monodon)) is a common practice in Region 3. Pond preparation is given importance prior to stocking of various species. Removal of sludge in the pond and application of probiotics are included in the standard pond preparation. The size and stocking densities of each species vary depending on the season. The volume of water to be replaced is based on the transparency of the water.The various species are fed natural food grown in the pond in addition to mollusks and low value fish given specifically for mud crab and shrimp. When pond water becomes transparent (indicating the decrease in phytoplankton density), the water is agitated using a small boat. If water continues to be transparent, fertilizers are applied to enhance growth of natural food. The pond water and animals are closely monitored to prevent diseases. Some of the early signs of occurrence of disease include the presence of bubbles on the water surface, and presence of weak shrimp on the feeding trays and along the dikes. The presence of weak shrimp and crab on the pond bottom and continuous swimming of these animals from night to dawn indicate poor pond water quality which can be remedied by circulating and flushing of the pond water every other day.
Development of protocol for the production of hatchery-reared mud crab Scylla serrata juveniles for soft-shell crab farming ET Quinitio, GX Libunao & FD Parado-Estepa - In ET Quinitio, FD Parado-Estepa & RM Coloso (Eds.), Philippines : In the forefront of the mud crab industry development : proceedings of the 1st National Mud Crab Congress, 16-18 November 2015, Iloilo City, Philippines, 2017 - Aquaculture Department, Southeast Asian Fisheries Development CenterDevelopment of economically viable techniques for growing hatchery-reared juvenile crabs to suitable sizes will address the problem on the source of seed stocks for soft-shell crab farming. This paper reports the production of hatchery-reared mud crab Scylla serrata from juveniles in the nursery to 73-106 g body weight (BW) crabs in the grow-out pond for the individual system soft-shell crab farming. Likewise, the performance of hatchery-reared S. serrata, and wild S. tranquebarica and S. olivacea juveniles was determined in the soft-shell crab production set-up.The BW increased from 1.8-1.9 g to 78-113.7 g when stocked at 0.5 ind m-2 and from 1.6-2.3 g to 73-106.7 g at 1.0 ind m-2 after 75 days. Growth rates at both stocking densities were comparable. However, survival was significantly higher (P<0.05) in lower (63.6~c1.01%) than in higher (35.6~c3.34%) stocking density. Male S. serrata (46.0 ~c 1.75%) had significantly higher BW increase than females (39.4 ~c 2.05%). Crabs stocked at sizes of 51-60 g showed significantly greater percent increase in BW (43.26~c 0.98%) compared with those at 61-70 g (40.98~c1.33%), 71-80 g (38.55~c 1.04%), 81-90 g (36.34 ~c 1.27%) and 91-100 g (38.52 ~c 1.67%). Among the three species, hatchery-reared S. serrata (42.14 ~c 1.34%) had significantly higher mean percent BW increase compared with S. olivacea (38.23 ~c 0.49%) and S. tranquebarica (36.16 ~c 0.78%). S. serrata had significantly shorter mean culture period (24.11 ~c 0.95 days) than S. tranquebarica (28.48 ~c 0.54 days) and S. olivacea (28.75 ~c 0.34 days).
Book | Conference publication
Philippines: In the forefront of the mud crab industry: Proceedings of the 1st National Mud Crab Congress ET Quinitio, FD Parado-Estepa & RM Coloso (Eds.) - In ET Quinitio, FD Parado-Estepa & RM Coloso (Eds.), Philippines : In the forefront of the mud crab industry development : proceedings of the 1st National Mud Crab Congress, 16-18 November 2015, Iloilo City, Philippines, 2017 - Aquaculture Department, Southeast Asian Fisheries Development Center