Grouper research at the Southeast Asian Fisheries Development Center Aquaculture Department
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This paper provides information on grouper research activities that have been carried out in SEAFDEC AQD. It covers various aspects such as broodstock management, seed production, nursery and grow-out culture techniques.
Marte, C. L. (2002). Grouper research at the Southeast Asian Fisheries Development Center Aquaculture Department. In Asia-Pacific Economic Cooperation & Network of Aquaculture Centres in Asia-Pacific (Eds.), Report of the APEC/NACA Cooperative Grouper Aquaculture Workshop, Hat Yai, Thailand 7-9 April 1999 (pp. 143-151). Bangkok, Thailand: Network of Aquaculture Centres in Asia-Pacific.
PublisherNetwork of Aquaculture Centres in Asia-Pacific
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Book chapterPS Eusebio, JD Toledo, REP Mamauag & MJG Bernas - In MA Rimmer, S McBride & KC Williams (Eds.), Advances in grouper aquaculture, 2004 - Australian Centre for International Agricultural Research
Series: ACIAR Monograph 110This study was undertaken to determine the activities of alkaline and acid type proteases [proteinases], α-amylase, lipase [triacylglycerol lipase], trypsin, chymotrypsin, leucine aminopeptidase [cytosol aminopeptidase], and alkaline and acid phosphatases during larval development of the grouper, Epinephelus coioides. The maximum variation in specific activities of alkaline and acid type proteases, α-amylase, lipase, trypsin, chymotrypsin, leucine aminopeptidase, and acid and alkaline phosphatases in the digestive tract of grouper larvae was mostly related to the onset or the end of metamorphosis during larval development.
Temporal changes in innate immunity parameters, epinecidin gene expression, and mortality in orange-spotted grouper, Epinephelus coioides experimentally infected with a fish pathogen, Vibrio harveyi JML1 EC Amar, JP Faisan Jr., MJS Apines-Amar & RV Pakingking Jr. -
Fish and Shellfish Immunology, 2017 - ElsevierChanges in innate immunity parameters and epinecidin mRNA transcript levels were examined to characterize the non-specific immune response of E. coioides to pathogenic V. harveyi JML1 isolated from affected cage-cultured fish. After fish had been injected with bacteria at a dose causing 30% mortality, blood and tissue samples were collected at 0, 6, 12, 24, 48, 72, 96, 120, and 240 h post-infection (hpi) for assessment of indices such as the oxidative burst (OB) and phagocytic index (PI) of head kidney cells, and lysozyme activity (LYS) and total immunoglobulin (Total Ig) levels of the plasma. The epinecidin mRNA transcript levels (EGE) from skin, gills, liver, kidney, and spleen tissues were also determined by gelbased RT-PCR. Lastly, daily mortality (DM), liver total bacterial load (TBC), and presumptive Vibrio count (TVC) were monitored up to 240 hpi. The results revealed that bacteria proliferated rapidly in fish tissue, reaching peak densities at 24 hpi for both TBC and TVC but was on a downward trend thereafter. The pattern in fish mortality closely correlated with TBC and TVC. Total Ig, OB, and PI in E. coioides were suppressed in the early part of infection when V. harveyi load was high but recovered and later increased as bacterial density declined. LYS and EGE were consistently high and their activities were not hampered by bacterial infection. The study demonstrated that V. harveyi JML1 interacts with E. coioides by transiently inhibiting some immune parameters resulting in mortalities. However, consistently high LYS, upregulated EGE, and resurgent PI, OB and Total Ig conferred resistance and subsequent recovery in the fish. The study provides new insights on the interaction between E. coioides and V. harveyi JML1 that can aid in formulating health management strategies for groupers. Further studies on prophylactic interventions to enhance the innate immune response in grouper during infection with V. harveyi JML1 are suggested.
ArticleGF Quinitio, JD Tan-Fermin & A Nagai -
Fisheries Science, 2001 - Japanese Society of Fisheries ScienceThirty immature juvenile grouper Epinephelus coioides (19-168 g bodyweight, BW) were randomly stocked in four units 6 t tanks to determine if mibolerone can be used to induce sex inversion in groupers. After acclimatization and weaning to artificial feed, the feed given daily (4% BW/day) was supplemented with 0, 50, 100, and 200 μg mibolerone/kg feed for about 18 weeks. Thereafter, the hormone treatment was withdrawn and the experiment was terminated at Week 24. Ten fish were killed for gonad histology at stocking to serve as an initial control while about three to five fish were killed every 8 weeks. In general, ovaries of initial controls showed the presence of moderate stromal cells and gonia and few primary oocytes. At Weeks 8 and 16, ovaries of the control fish (0 μg/kg) were similar to that of the initial control except that primary oocytes increased at Week 24. Gonads of fish fed diets containing 100 and 200 μg/kg had none to moderate spermatocytes and few spermatids at Week 8 and 16, although spermatozoa were not observed, indicating that the fish were undergoing spermatogenesis. Spermatogenesis at 50 μg/kg was not as advanced since only few spermatocytes occurred at Weeks 8 followed by moderate gonia and no spermatocytes and spermatids at Week 16. However, the presence of few primary oocytes was observed when mibolerone was withdrawn suggesting that sex-inversed fish reverted back to a female condition. These results show that sex inversion in juvenile grouper can be induced by oral administration of mibolerone and may have possible application on mature females to produce functional males.