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Development of a giant grouper Luteinizing Hormone (LH) Enzyme-Linked Immunosorbent Assay (ELISA) and its use towards understanding sexual development in grouper

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วันที่
2020-07-13
ผู้เขียน
Peter Dennis, Lachlan
Nocillado, Josephine
Palma, Peter ORCID
Amagai, Takafumi
Soyano, Kiyoshi
Elizur, Abigail
Page views
3,534
เรื่อง
reproduction ASFA
ELISA ASFA
luteinizing hormone AGROVOC
groupers AGROVOC
LH AGROVOC
Reproductive biology
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นามธรรม
A recombinant giant grouper Luteinizing Hormone (LH) consisting of tethered beta and alpha subunits was produced in a yeast expression system. The giant grouper LH β-subunit was also produced and administered to rabbits for antibody development. The recombinant LH and its antibody were used to develop an Enzyme Linked Immunosorbent Assay (ELISA). This ELISA enabled detection of plasma LH levels in groupers at a sensitivity between 391 pg/ml and 200 ng/ml. Different species of grouper were assayed with this ELISA in conjunction with gonadal histology and body condition data to identify links between circulating LH levels and sexual development. We found that circulating levels of LH decreased when oocytes began to degenerate, and sex-transition gonadal characteristics were apparent when LH levels decreased further. When circulating LH levels were related to body condition (body weight/ body length), transitioning-stage fish had relatively high body condition but low plasma LH levels. This observation was similar across multiple grouper species and indicates that plasma LH levels combined with body condition may be a marker for early male identification in the protogynous hermaphrodite groupers.
URI
http://hdl.handle.net/10862/5901
การอ้างอิง
Peter Dennis, L., Nocillado, J., Palma, P., Amagai, T., Soyano, K., & Elizur, A. (2020). Development of a giant grouper Luteinizing Hormone (LH) Enzyme-Linked Immunosorbent Assay (ELISA) and its use towards understanding sexual development in grouper. General and Comparative Endocrinology, 296, 113542. https://doi.org/10.1016/j.ygcen.2020.113542 
DOI
10.1016/j.ygcen.2020.113542
Type
Article
ISSN
0016-6480
คอลเลกชัน
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