Studies on the efficacy of Sarafin® (sarafloxacin hydrochloride) on vibrios associated with vibriosis in black tiger shrimp (Penaeus monodon)
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In: Lavilla-Pitogo, C. R., Cruz-Lacierda, E. R. (Eds.). Diseases in Asian Aquaculture IV. Proceedings of the Fourth Symposium on Diseases in Asian Aquaculture, 22-26 November 1999, Cebu City, Philippines. Quezon City, Philippines: Fish Health Section, Asian Fisheries Society. pp. 125-134
PublisherFish Health Section, Asian Fisheries Society
Crustacea; Shrimp culture; Bacterial diseases; Disease control; Toxicity tolerance; Bioassays; Bacteriocides; Philippines; Shrimps; Bacteria; Giant tiger shrimp; Penaeus monodon; Vibrio alginolyticus; Vibrio anguillarum; Vibrio spp.; Vibrio harveyi; Vibrio parahaemolyticus; Vibrio vulnificus; Vibrio mimicus
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ArticleFrom January 1990 to June 1993, 59% of the total juvenile to adult shrimp (Penaeus monodon) submitted for diagnosis at the Fish Health Section of the Aquaculture Department, Southeast Asian Fisheries Development Center, Tigbauan, Iloilo, Philippines were diagnosed to have red disease syndrome. Red disease syndrome is characterized by the reddening of the shrimp body. The aetiology of the disease is unknown. This paper reports for the first time the isolation of four Vibrio phenotypes, namely, Vibrio harveyi, V. parahaemolyticus, V. fluuialis and Vibrio sp. from shrimps with red disease. Pathogenicity test shows that injection with V. parahaemolyticus and V. harveyi can produce the characteristic red discoloration in healthy shrimp.
Current status of acute hepatopancreatic necrosis disease (AHPND) and other transboundary diseases of farmed shrimps in Indonesia MS Hastuti & Desrina - In RV Pakingking Jr., EGT de Jesus-Ayson & BO Acosta (Eds.), Addressing Acute Hepatopancreatic Necrosis Disease (AHPND) and Other Transboundary Diseases for Improved Aquatic … Diseases for Improved Aquatic Animal Health in Southeast Asia, 22-24 February 2016, Makati City, Philippines, 2016 - Aquaculture Department, Southeast Asian Fisheries Development CenterTransboundary diseases have been a constant challenge for the aquaculture industry in Indonesia. In spite of this, Indonesian aquaculture has experienced a steady growth since 2010. Early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) is a serious emerging transboundary disease of cultured shrimp that has not been reported in Indonesia. On the contrary, hepatopancreatic microsporidiosis (HPM) was first detected in 2015. Other previously reported transboundary diseases of shrimp and fish include white spot disease (WSD), Taura syndrome and infectious myonecrosis (IMN), and viral nervous necrosis (VNN) and koi herpesvirus (KHV), respectively. These diseases have been included in the surveillance program conducted in 2016. To avert the spread of these transboundary pathogens in the Indonesian aquaculture facilities and natural waters, competent authorities have been tasked to implement stringent control measures including government policy and regulation, active and passive surveillance, and strengthening farmers and stake holders awareness of the importance of disease control and health maintenance.
Conference paperI Hirono, S Tinwongger, Y Nochiri & H Kondo - In RV Pakingking Jr., EGT de Jesus-Ayson & BO Acosta (Eds.), Addressing Acute Hepatopancreatic Necrosis Disease (AHPND) and Other Transboundary Diseases for Improved Aquatic … Diseases for Improved Aquatic Animal Health in Southeast Asia, 22-24 February 2016, Makati City, Philippines, 2016 - Aquaculture Department, Southeast Asian Fisheries Development CenterAcute hepatopancreatic necrosis disease (AHPND) is caused by unique strains of Vibrio parahaemolyticus (VPAHPND) and V. harveyi that have transferrable plasmid carrying the virulent PirAB-like toxin genes. The genomes of VPAHPND strains and V. harveyi from Thailand and Viet Nam, respectively, have been characterized by our group. The genome of VPAHPND strains from Mexico, Viet Nam, and China have also been studied by other groups. We have developed a conventional polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) methods for the detection of AHPND using a primer set that targets the PirAB-like toxin genes of VPAHPND. We have characterized the toxin genes of VPAHPND strains and also constructed a recombinant plasmid (broad host range) carrying PirAB-like toxin genes. Non-VPAHPND strain N7 which does not carry the plasmid and strain FP11 which is carrying a plasmid not coding for the toxin genes were transformed with the plasmid carrying PirAB-like toxin genes. As a result, the transformed N7 and FP11 strains became virulent and killed whiteleg shrimp (Penaeus vannamei) similar to or at par with the virulence of VPAHPND strain. We then fed the whiteleg shrimp with commercial feed containing the formalin-killed VPAHPND strain. After 2 days of feeding, all of the whiteleg shrimp died. These results clearly indicate that the PirAB-like toxin is the virulence factor of VPAHPND. We have been investigating the virulence mechanism of the PirAB-like toxin produced by VPAHPND strains. First, we calculated the copy number of plasmid encoding the PirAB-like toxin genes of several VPAHPND strains. The copy number of the plasmid varied, ranging from 1 to 36 copies. Interestingly, VPAHPND strains carrying low copy number of plasmid were more virulent than VPAHPND strains carrying high copy number of the plasmid. These results imply that the copy number of toxin genes is not an important factor responsible for the degree of virulence of the VPAHPND strains. We are also studying other factors associated with the virulence of PirAB-like toxin. Likewise, we are developing prevention methods against AHPND including the use of formalin-killed cell vaccine, IgY additive in feed, and nano-bubble treatment of rearing water. This paper summarizes the current R&D on the disease.