Acute hepatopancreatic necrosis disease (AHPND) outbreaks in Penaeus vannamei and P. monodon cultured in the Philippines
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Acute hepatopancreatic necrosis disease (AHPND) has recently emerged as a serious disease of cultured shrimp. It has also been described as early mortality syndrome (EMS) due to mass mortalities occurring within 20 to 30 d after stocking of ponds with postlarvae. Here, Penaeus vannamei and Penaeus monodon from shrimp farms in the Philippines were examined for the toxin-producing strain of Vibrio parahaemolyticus due to AHPND-like symptoms occurring in marketable size shrimp. In the P. vannamei, histology revealed typical AHPND pathology, such as sloughing of undifferentiated cells in the hepatopancreatic tubule epithelium. Analysis using the IQ2000 AHPND/EMS Toxin 1 PCR test generated 218 bp and 432 bp amplicons confirmative of the toxin-producing strain of V. parahaemolyticus among shrimp sampled from 8 of 9 ponds. In the P. monodon, histology revealed massive sloughing of undifferentiated cells of the hepatopancreatic tubule epithelium in the absence of basophilic bacterial cells. PCR testing generated the 2 amplicons confirmatory for AHPND among shrimp sampled from 5 of 7 ponds. This study confirms the presence of AHPND in P. vannamei and P. monodon farmed in the Philippines and suggests that the disease can also impact late-stage juvenile shrimp.
Citationde la Peña, L. D., Cabillon, N. A. R., Catedral, D. D., Amar, E. C., Usero, R. C., Monotilla, W. D., ... Saloma, C. P. (2015). Acute hepatopancreatic necrosis disease (AHPND) outbreaks in Penaeus vannamei and P. monodon cultured in the Philippines.
We are very grateful to DOST-PCAARRD and SEAFDEC/AQD for funding this work and to the Shrimp Pathogenomics team, especially Dr. N. R. L. Rojas and Dr. E. P. Enriquez. We thank the shrimp farms that provided the samples and the staff of Fish Health Section Diagnostic Services and Microtechnique Lab for the technical support. We also thank T. W. Flegel for confirming our histological analysis and for assistance in editing the manuscript.
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Conference paperF Piedad-Pascual - In RD Fortes, LC Darvin & DL de Guzman (Eds.), Fish and Crustacean Feeds and Nutrition. Proceedings of the Seminar-Workshop on Fish and Crustacean Feeds and Nutrition, 25-26 February 1985, Iloilo City, Philippines, 1989 - Philippine Council for Aquatic and Marine Research and DevelopmentThis paper echoes what transpired during the first International Conference of Penaeid Prawns/Shrimps held in Iloilo City in December 4-7, 1984, particularly on the Nutrition nd Feed Development. Around 25 papers were presented during the conference. The nutrient requirements of P. japonicus and to some extent, P. monodon have been studied quite extensively compared to other penaeid species. Requirements for protein, carbohydrates fats, amino acids and essential fatty acids for juveniles and larvae have been defined compared to those of the broodstock. Optimum protein levels for prawn juveniles vary from 28-38% for P. kerathurus, 40-46% for P. monodon, 43% for P. indicus and 50-54% for P. japonicus. Dissacharides like sucrose and trehalose have been found to be good source of carbohydrates at 20-25% in the diet. Crustacean diets require around 0.5% cholesterol. There are few studies on vitamine and mineral requirements. There are artificial diets for juveniles and microencapsulated diets that can completely replace live organisms as larval feed. Microencapsulated diets have been field-tested for P. vannamei, P. stylirostris, P. monodon, P. indicus and P. merguiensis in Ecuador, Taiwan, Malaysia, Thailand and the Philippines. When a commercial diet for the broodstock becomes available ther will be an artificial diet for athe life cycle of tha prawn.
Development of farming schemes following disease occurrences in monodon shrimp farming using intensive method in three Southeast Asian countries T Matsuura, LD de la Peña, CP Ean, R Siow & AH Alias - In T Matsuura (Ed.), Comparative Analysis of Aquaculture Management in Brackish Mangrove Areas in Three Southeast Asian Countries, 2007 - Japan International Research Center for Agricultural Sciences
Series: JIRCAS Working Report; No. 56All three countries experienced major progress in intensive shrimp farming at different ties. Intensive farming of monodon shrimp (Penaeus monodon, an indigenous species) was first launched by the Philippines, followed by Thailand, then by Peninsular Malaysia. The survey was conducted from 2000-2005. The disease-causing bacterial that seiously damaged monodon culture were luminous bacteria in the Philippines and white spot virus in Thailand and Malaysia. Production decreased because of these diseases in the mid-1990s in the Philippines and after 2000 in Thailand and Malaysia. In 1998, the Green Water System (hereinafter referred to as GWS) was developed, and a proportion of culture ponds introduced it and resumed monodon culture using the intensive method. In Thailand, the vannamei shrimp (Penaeus vannamei, an exotic species from South America) has now replaced monodon previously raised using the extensive method. In Malaysia, monodon is cultured using only the intensive method, and some culture pond enterprises started to culture vannamei instead of monodon in 2004. In the Philippines, companies engaging in monodon culture have many ponds and lower stocking density because they culture large-size shrimp, but are exposed to high running costs such as fuels and probiotics. On the other hand, in Thailand, individuals engaging in monodon culture have few ponds and higher stocking density since they culture small-scale shrimp. Labor costs are low because most of the work is done by family member.
Current status and impact of early mortality syndrome (EMS)/acute hepatopancreatic necrosis disease (AHPND) and hepatopancreatic microsporidiosis (HPM) outbreaks on Thailand s shrimp farming S Putth & J Polchana - In RV Pakingking Jr., EGT de Jesus-Ayson & BO Acosta (Eds.), Addressing Acute Hepatopancreatic Necrosis Disease (AHPND) and Other Transboundary Diseases for Improved Aquatic … Diseases for Improved Aquatic Animal Health in Southeast Asia, 22-24 February 2016, Makati City, Philippines, 2016 - Aquaculture Department, Southeast Asian Fisheries Development CenterOutbreak of early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) in whiteleg shrimp (Penaeus vannamei) and black tiger shrimp (P. monodon) was first documented in August 2011 in Eastern Thailand. The disease subsequently spread to almost all shrimp production areas in 2012 until the early part of 2016. These episodes of AHPND outbreaks consequently impacted the shrimp industry as evidenced by significant reduction in the production of farmed shrimps, shortage of raw materials for the shrimp export industry, and reduction of global shrimp supply from Thailand. Following the discovery of Vibrio parahaemolyticus as the causal agent of AHPND, PCR techniques subsequently became available for the rapid and accurate detection of AHPND in cultivated shrimps. The Department of Fisheries (DOF) consequently included AHPND in the National Surveillance Program focusing on the investigation of risk factors responsible for the outbreak and concomitant spread of the disease. As a result, the quality of broodstock and postlarvae (PL) and as well as farm management practices, i.e. pond bottom and water preparation, stocking density, feeds and feeding practices, and water quality fluctuations were identified as key risk factors associated with AHPND outbreaks. By and large, the DOF has undertaken mitigating measures to control and prevent further outbreaks of AHPND including the improvement of sanitation in marine shrimp broodstock and PL hatcheries, quality evaluation and disease screening of broodstocks and PL, detection of pathogens in soil and water samples, and acquisition of new broodstocks for improved genetic diversity. To date, Thailand s shrimp industry has gradually recovered from the devastating effects of AHPND since 2015. Heptopancreatic microsporidiosis (HPM) caused by Enterocytozoon hepatopenaei (EHP), a sporeforming microsporidian, is another emerging disease of cultured penaeids in Thailand. EHP was first documented in farmed P. monodon in 2004, however, its impact was not clearly evaluated at that time. EHP was again observed in P. vannamei in 2014 at the same period of AHPND outbreak in Thailand. In the field, EHP could be transmitted horizontally through feeding of the EHPcontaminated feed and feces from infected shrimp. Samples collected from numerous shrimp farms showed that EHP was heavily present in both ponds with successful and failed crops indicating that EHP infection in shrimp may not be a significant contributing factor to a failed production run. In vitro challenge likewise showed that there was no correlation between EHP and white feces syndrome. However, EHP infection at significantly high levels could affect shrimp growth. One of the mitigating measures to control EHP infection in cultured shrimp is the reduction of contamination in hatcheries and grow-out facilities. Despite the negative impacts of AHPND and HPM on the shrimp industry of Thailand, all parties of the shrimp sector have been working in concert to attain the projected annual shrimp production volume of approximately 300,000 metric tons (MT) in 2016.