Induced spawning and larval rearing of grouper (Epinephelus salmoides Maxwell)
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Broodfish of grouper (Epinephelus salmoides ) were induced to spawn by hormonal induction. The hormones used for this experiment were HCG + pituitary gland (PG) and LRH-a. The results showed that at dosages of 500 IU HCG + 3 mg of PG per kg of fish for first injection and 1,000 IU HCG + 3 mg of PG per kg of fish at the final injection at an interval of 24 hours, the treated fish spawned naturally in a spawning tank 12 hours after the final injection. At lower dosages of 500 IU HCG + 3 mg PG at 12-hour intervals or 500 IU HCG + 3 mg PG at 24-hour intervals or using 10 mg LRH-a at 12-hour intervals, the eggs can be artificially fertilized only by stripping. The larval rearing experiment was conducted in a 250-l fiberglass tank at the stocking density of 2,500 larvae per tank. Feeds used in this experiment were: (a) Isochrysis + sea urchin eggs; (b) Isochrysis + Brachionus ; and (c) Tetraselmis + Brachionus . The results showed that newly hatched larvae fed with Isochrysis mixed with sea urchin eggs and then Brachionus from day 10 had the best survival rate (9% at day 30).
Excerpt from The First Asian Fisheries Forum. Asian Fisheries Society, Manila, Philippines.
The First Asian Fisheries Forum. 663-666
- Conference Proceedings 
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ArticleEA Tendencia -
Bulletin of the European Association of Fish Pathologists, 2004 - European Association of Fish PathologistsAt SEAFDEC AQD, opaque spawned grouper eggs are observed during collection in cage-reared brood stock; while opaque and multi-colored eggs are often observed in tank-reared fishes. This study aimed to investigate the occurrence of these opaque and multicolored eggs and at the same time to compare the bacterial microbiota of eggs from brood stock reared in cages, to those from fish reared in concrete tanks. Grouper eggs from brood stocks reared in cages and tanks were processed for bacterial count and identification. Results showed that total bacterial count (on MA) and presumptive Vibrio count (on TCBS) of eggs from brood stock reared in concrete tanks were lower than those from cage-reared fishes. Aeromonads (for tank-reared) and Pseudomonads (for cage-reared) were the dominant bacteria in the good eggs; while Vibrios were dominant in the bad eggs for both egg sources. Total bacterial count of the egg-incubating medium from the brood stock tanks (104 cfu/ml) was lower than the total bacterial count of water from the cages (107 cfu/ml). Presumptive Vibrio counts of water from the tanks (102 cfu/ml) were lower than those from the cages (106 cfu/ml). The Aeromonads dominated the water from the tanks; while Vibrios dominated those from the cages. Good eggs that did not hatch, turned yellow after 3 days, and pink after 5 days.
Conference paperMN Duray - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development CenterThe feeding habits of hatchery-reared Epinephelus suillus larvae were determined by examining their gut contents. The larvae (2.6 mm TL) were initially fed rotifers on day 2 and newly-hatched Artemia nauplii on day 21 (9.1 mm TL). The amount of rotifers initially ingested averaged 1.3 individuals/larva. The ingestion rate increased as larvae grew. Larvae immediately showed strong preference for Artemia to rotifers on the first day of introduction. E. suillus larvae showed diurnal feeding pattern at day 7 (3.6 mm TL), day 14 (4.9 mm TL), day 21 (9.1 mm TL) and day 28 (11.1 mm TL). Feeding incidence decreased in the evening and was nil at 2100-2200 h. Active feeding started earlier in older larvae and satiation was between 0900-1000 h. The results of this study will be used as a basis in developing a good feeding scheme for E. suillus larvae.
Daily rates of ingestion on rotifers and Artemia nauplii by laboratory-reared grouper larvae of Epinephelus suillus MN Duray -
The Philippine Scientist, 1994 - San Carlos Publications, University of San CarlosThe amount of rotifers and Artemia nauplii consumed daily by the grouper Epinephelus suillus larvae was determined through examination of gut contents. The larvae (2.6 mm TL) were initially fed rotifers on day 2 and newly hatched Artemia nauplii on day 21 (9.1 mm TL). The amount of food consumed by the larvae increased with larval growth. Larvae also showed diurnal feeding pattern at day 7 (3.6 mm TL), day 14 (4.9 mm TL), day 21 (9.1 mm TL) and day 28 (11.1 mm TL). Feeding incidence increased in the evening and became zero at 2100-2200 h. Active feeding started earlier in the older larvae and satiation was between 0900-1100 hr.