Induced spawning and larval rearing of grouper (Epinephelus salmoides Maxwell)
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Broodfish of grouper (Epinephelus salmoides ) were induced to spawn by hormonal induction. The hormones used for this experiment were HCG + pituitary gland (PG) and LRH-a. The results showed that at dosages of 500 IU HCG + 3 mg of PG per kg of fish for first injection and 1,000 IU HCG + 3 mg of PG per kg of fish at the final injection at an interval of 24 hours, the treated fish spawned naturally in a spawning tank 12 hours after the final injection. At lower dosages of 500 IU HCG + 3 mg PG at 12-hour intervals or 500 IU HCG + 3 mg PG at 24-hour intervals or using 10 mg LRH-a at 12-hour intervals, the eggs can be artificially fertilized only by stripping. The larval rearing experiment was conducted in a 250-l fiberglass tank at the stocking density of 2,500 larvae per tank. Feeds used in this experiment were: (a) Isochrysis + sea urchin eggs; (b) Isochrysis + Brachionus ; and (c) Tetraselmis + Brachionus . The results showed that newly hatched larvae fed with Isochrysis mixed with sea urchin eggs and then Brachionus from day 10 had the best survival rate (9% at day 30).
Excerpt from The First Asian Fisheries Forum. Asian Fisheries Society, Manila, Philippines.
- Conference Proceedings 
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Tendencia, E. A. (European Association of Fish Pathologists, 2004)At SEAFDEC AQD, opaque spawned grouper eggs are observed during collection in cage-reared brood stock; while opaque and multi-colored eggs are often observed in tank-reared fishes. This study aimed to investigate the ...
Daily rates of ingestion on rotifers and Artemia nauplii by laboratory-reared grouper larvae of Epinephelus suillus Duray, Marietta N. (San Carlos Publications, University of San Carlos, 1994)The amount of rotifers and Artemia nauplii consumed daily by the grouper Epinephelus suillus larvae was determined through examination of gut contents. The larvae (2.6 mm TL) were initially fed rotifers on day 2 and newly ...
Duray, Marietta N. (Aquaculture Department, Southeast Asian Fisheries Development Center, 1996)The feeding habits of hatchery-reared Epinephelus suillus larvae were determined by examining their gut contents. The larvae (2.6 mm TL) were initially fed rotifers on day 2 and newly-hatched Artemia nauplii on day 21 (9.1 ...