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  • Breeding and Seed Production of Cultured Finfishes in the Philippines
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Effects of single injection of carp pituitary extract and human chorionic gonadotropin on germinal vesicle migration and ovulation in lampan sungai, Puntius schwanenfeldii (Bleeker): A preliminary study

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bspcfp_p078-082.pdf (70.47Kb) Open Access
Downloads: 3,077
Date
1996
Author
Harmin, Sharr A.
Joannes, R.
Cheah, S. H.
Page views
4,797
Subject
ovulation ASFA
sexual maturity ASFA
fish eggs ASFA
embryonic development ASFA
oocytes ASFA
experimental culture ASFA
controlled conditions ASFA
induced ovulation ASFA
freshwater fishes ASFA
oogenesis ASFA
sex hormones ASFA
Malaysia AGROVOC
Taxonomic term
Puntius schwanenfeldii GBIF
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Abstract
The effects of crude pituitary extract (CPE) and human chorionic gonadotropin (hCG) on germinal vesicle migration and ovulation in lampan sungai Puntius schwanenfeldii (Bleeker) were investigated. Sexually mature female lampan sungai were divided into four groups (n=3 per group). In both experiments, fish were injected intramuscularly (im) once and were checked for germinal vesicle migration and sign of ovulation 8 h following injection. In experiment 1, females were treated with CPE at doses of 2, 4, or 6 mg/kg body weight (BW) while controls were administered an equivalent volume of saline. Ovulation occurred in one fish given 2 mg/kg and in another fish that received 6 mg/kg CPE. In a second experiment, female fish were administered hCG at doses of 1000, 2000, or 4000 IU/kg body weight. Similarly, controls received only saline. One female injected with 4000 IU/kg hCG responded positively to the hormone treatment. Clearly, in our study, a single intramuscular injection of CPE and hCG induced spawning in lampan sungai as well as accelerated oocyte development as evidenced by the increasing percentage of oocytes showing germinal vesicle migration.
URI
http://hdl.handle.net/10862/589
Suggested Citation
Harmin, S. A., Joannes, R., & Cheah, S. H. (1996). Effects of single injection of carp pituitary extract and human chorionic gonadotropin on germinal vesicle migration and ovulation in lampan sungai, Puntius schwanenfeldii (Bleeker): A preliminary study. In C. L. Marte, G. F. Quinitio, & A. C. Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993 (pp. 78-82). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
Type
Conference paper
ISBN
9718511326
Collections
  • Breeding and Seed Production of Cultured Finfishes in the Philippines [31]

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