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Standardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon)

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delaPenaLD2005.pdf (477.0Kb) AQD Access AQD Access
Downloads: 7
Date
2005-03
Author
de la Peña, Leobert D.
Lavilla-Pitogo, Celia R.
Paner, Milagros G.
Page views
2,346
Subject
White spot syndrome virus ASFA
standardizing ASFA
polymerase chain reaction ASFA
disease recognition ASFA
animal diseases ASFA
DNA ASFA
freshwater crustaceans ASFA
shrimp culture ASFA
RNA ASFA
Penaeus monodon AGROVOC
Episesarma versicolor AGROVOC
Penaeus japonicus AGROVOC
Taxonomic term
Penaeus monodon GBIF
Metadata
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Abstract
Several runs were conducted using the three published PCR protocols and the three DNA extraction methods. All three primer pairs designed by individual authors based on the sequence of a cloned fragment of the white spot syndrome virus (WSSV) genome successfully detected the Philippine WSSV isolates. In addition, a high specificity of the assay was also obtained. CTAB and DNAzol extraction methods consistently gave a better DNA quality using the three PCR protocols. Any of the three PCR protocols can be used in the rapid and sensitive detection of WSSV in shrimp (Penaeus monodon) and crab (Episesarma vesicolor).
URI
http://hdl.handle.net/10862/5907
Suggested Citation
de la Peña, L. D., Lavilla-Pitogo, C. R., & Paner, M. G. (2005). Standardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon). In K. Nagasawa (Ed.), Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia (pp. 19–26). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
Type
Book chapter
ISBN
9718511732
Collections
  • Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia [43]

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