Standardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon)
AQD Access
Date
2005-03Page views
2,143ASFA keyword
AGROVOC keyword
Taxonomic term
Metadata
Show full item recordShare
Abstract
Several runs were conducted using the three published PCR protocols and the three DNA extraction methods. All three primer pairs designed by individual authors based on the sequence of a cloned fragment of the white spot syndrome virus (WSSV) genome successfully detected the Philippine WSSV isolates. In addition, a high specificity of the assay was also obtained. CTAB and DNAzol extraction methods consistently gave a better DNA quality using the three PCR protocols. Any of the three PCR protocols can be used in the rapid and sensitive detection of WSSV in shrimp (Penaeus monodon) and crab (Episesarma vesicolor).
Suggested Citation
de la Peña, L. D., Lavilla-Pitogo, C. R., & Paner, M. G. (2005). Standardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon). In K. Nagasawa (Ed.), Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia (pp. 19–26). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
Type
Book chapterISBN
9718511732Related items
Showing items related by title, author, creator and subject.
-
An overview of the nutrition, feed and feeding techniques of prawn penaeid/shrimps
(Philippine Council for Aquatic and Marine Research and Development, 1989)This paper echoes what transpired during the first International Conference of Penaeid Prawns/Shrimps held in Iloilo City in December 4-7, 1984, particularly on the Nutrition nd Feed Development. Around 25 papers were presented during the conference. The nutrient requirements of P. japonicus and to some extent, P. monodon have been studied quite extensively compared to other penaeid species. Requirements for protein, carbohydrates fats, amino acids and essential fatty acids for juveniles and larvae have been defined compared to those of the broodstock. Optimum protein levels for prawn juveniles vary from 28-38% for P. kerathurus, 40-46% for P. monodon, 43% for P. indicus and 50-54% for P. japonicus. Dissacharides like sucrose and trehalose have been found to be good source of carbohydrates at 20-25% in the diet. Crustacean diets require around 0.5% cholesterol. There are few studies on vitamine and mineral requirements. There are artificial diets for juveniles and microencapsulated diets that can completely replace live organisms as larval feed. Microencapsulated diets have been field-tested for P. vannamei, P. stylirostris, P. monodon, P. indicus and P. merguiensis in Ecuador, Taiwan, Malaysia, Thailand and the Philippines. When a commercial diet for the broodstock becomes available ther will be an artificial diet for athe life cycle of tha prawn. -
Series: Aquaculture extension manual; No. 19
Prawn hatchery operations
; Quinitio, Emilia T.
; (Aquaculture Department, Southeast Asian Fisheries Development Center, 1996-05)
The manual, an updated version of the 1984 SEAFDEC/AQD manual, presents the underlying principles and step-by-step instructions of prawn larval and post-larval rearing. The techniques described are not only applicable to Penaeus monodon, but may also be modified and applied to other penaeid and metapenaeid species. The following aspects are covered: Site selection; Life cycle; Hatchery design and planning; Hatchery operations; Diseases; and Economics. -
The lowdown on world shrimp culture - II
(INFOFISH, 2001)This paper introduces some new members of the international shrimp culture club and goes on to discuss some recent technological innovations in the industry, particularly the polyculture of tilapia (mainly Oreochromis mossambicus) and shrimp.
