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Nursery and grow-out culture of the abalone Haliotis asinina on a reef flat: A comparison of growth and survival using different culture containers

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Date
2021-08
Author
Lebata-Ramos, Ma. Junemie Hazel ORCID
Mediavilla, Jonas P.
Solis, Ellen Flor
Sibonga, Rema ORCID
Alicante, Frances Patrick L.
Dionela, Cleresa S.
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1,633
ASFA keyword
abalone culture ASFA
growing ponds ASFA
growth ASFA
nursery ponds ASFA
grow-out ASFA
survival ASFA
water temperature ASFA
containers ASFA
AGROVOC keyword
abalones AGROVOC
Haliotis asinina AGROVOC
Culture container
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Abstract
The study was conducted to compare four different culture containers [cage, recycled oil container, tray (control), and tube] in the nursery and grow-out culture of the abalone Haliotis asinina on a reef flat. For nursery culture, early juvenile abalone with mean shell length (SL) of 1.30 ± 0.006 cm and mean body weight (BW) of 0.45 ± 0.007 g were stocked in the containers at 100 ind m−2 and reared until they attained the 3 cm SL size required for grow-out culture in three of the four culture containers. For grow-out culture, juvenile abalone harvested from nursery culture with mean SL of 3.23 ± 0.018 cm and mean BW of 7.33 ± 0.131 g were stocked in the containers at 50 ind m−2 and reared until they were harvestable at 5 cm SL cocktail size in three of the four culture containers. The 3 cm SL grow-out size was attained at 90 days of culture (DOC) in recycled containers, trays, and tubes, while the 5 cm SL cocktail size at 180 DOC in the same containers. Abalone reared in tubes had the highest mean SL after 90 DOC in nursery culture (3.21 cm), while those in trays after 180 DOC in grow-out culture (5.30 cm). However, at the end of the nursery and grow-out culture, there were no significant differences in mean SL among the abalone reared in these three culture containers. In both culture phases, abalone reared in cages had the lowest mean SL, 2.88 and 4.44 cm, respectively. Survival was highest in trays (98.64%) during nursery culture and in tubes (96.57%) during grow-out culture. With comparable results in recycled containers, trays, and tubes for both growth and survival at the nursery and grow-out phases, tubes are recommended for use when culture is to be done on reef flats. Tubes are the most stable and durable among the four culture containers tested. They can withstand strong winds and waves and may be reused for several culture runs.
URI
http://hdl.handle.net/10862/6060
Suggested Citation
Lebata-Ramos, M. J. H., Mediavilla, J. P., Solis, E. F., Sibonga, R., Alicante, F. P. L., & Dionela, C. S. (2021). Nursery and grow-out culture of the abalone Haliotis asinina on a reef flat: A comparison of growth and survival using different culture containers. Aquaculture, 541, 736786. https://doi.org/10.1016/j.aquaculture.2021.736786 
DOI
10.1016/j.aquaculture.2021.736786
Type
Article
ISSN
0044-8486
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  • Journal Articles [1267]

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    Grow-out culture of the abalone Haliotis asinina in Guimaras, Philippines: Comparison of tubes and drums as culture containers and the effects of serpulid infestation 

    Lebata-Ramos, Ma. Junemie Hazel ORCID; Solis, Ellen Flor ORCID (Springer, 2025-02-06)
    This study compared tubes and drums as containers for the grow-out culture of abalone Haliotis asinina using mixed seaweeds and formulated diet as feed. The recycled drums were newly tested containers for abalone culture as the cheaper and larger alternative. However, on the 10th month of culture, serpulids (Pomatoceros sp. and Spirorbis sp.) were observed on the abalone shells; hence, the 12-month culture was extended to 16 months to observe further the effects of these ectoparasites on the growth and survival of abalone. Throughout the culture period, abalone reared in tubes had significantly higher mean shell length and body weight than those reared in drums, measuring 45.42 ± 0.36 mm, 23.40 ± 0.99 g, and 41.45 ± 0.23 mm, 18.26 ± 0.40 g at harvest, respectively. Mean growth rates of those in tubes were significantly higher than those in drums before serpulid infestation but did not differ during months of infestation. Survival was consistently higher in tubes than drums, except during May–September 2023. At harvest, survival in tubes was 46.7 ± 11.0%, 37.8 ± 5.9% in drums. Daily water temperature ranged 28.00–32.33 °C, salinity 16.00–34.37 ppt. Monthly readings (ppm) of dissolved oxygen ranged 3.1–7.4, sulfide 0.00–0.73, phosphate 0.01–0.87, nitrate 0.00–0.99, nitrite 0.00–0.22, and total suspended solids 0.22–4.83. In April–October 2023, serpulids became prevalent (tubes = 31.5 ± 3.4%; drums = 38.3 ± 3.4%) when mean DO was significantly lower and sulfide higher than in June 2022-March 2023. These fluctuations in water parameters may have triggered the infestation, resulting in lower growth rates and abalone survival. But, despite these fluctuations and infestations, tubes remained a better culture container for the abalone.
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    Grow-out culture of tropical abalone, Haliotis asinina (Linnaeus) in suspended mesh cages with different shelter surface areas 

    Fermin, Armando C.; Buen, Shela Mae (Kluwer Academic Publishers, 2001)
    This study investigated the effects of shelter surface area (SSA) on the feeding, growth and survival of the donkey-ear abalone, Haliotis asinina reared in mesh cages (0.38×0.38×0.28m) suspended in flow-through tanks (water volume = 6 m3). Cages had sections of polyvinylchloride (PVC) that provided shelters with surface area of 0.22 m2, 0.44 m2 and 0.66 m2. Hatchery-produced abalone with initial shell length of 32 ± 1 mm and wet weight of 7.5 g were stocked at 50 individuals cage−1 that corresponded to stocking densities of ca. 227, 113 and 75 abalone m−2 of SSA. The ratios of shelter surface area to cage volume (SSA:CV) were 5.5, 11 and 16.5. Abalones were provided an excess red seaweed Gracilariopsis bailinae (= Gracilaria heteroclada) at weekly intervals over a 270-day culture period. Feeding rates (18–20% of wet weight), food conversion ratio (26–27) and percent survival (88–92%) did not differ significantly among treatments (p > 0.05). Body size at harvest ranged from 56 to 59 mm SL and 52 to 57 g wet body weight with significant differences between abalone reared at SSA 0.22 m2 and 0.66 m2 (p < 0.05). Abalone reared in cages with 0.66 m2 SSA grew significantly faster at average daily growth rates of 132 μm and 188 mg day−1. Stocking densities of 75–113m−2 SSA in mesh cages suspended in flow-through tanks resulted in better growth of abalone fed red seaweed.
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    Evaluation of density and cage design for the nursery and grow-out of the tropical abalone Haliotis asinina Linne 1758 

    Encena II, Vincent C.; de la Peña, Milagros; Balinas, Vicente T. (National Shellfisheries Association, 2013)
    The effect of stocking density and cage design on the growth, survival rate, and feed conversion ratio was evaluated for the nursery (11–15 mm in shell length) and juvenile grow-out (26–30 mm in shell length) of the tropical abalone Haliotis asinina. Abalone were fed Gracilaria sp. within a randomized 2 × 3 factorial experiment using 2 stocking densities (Tl (500 pieces/m2) and T2 (1,000 pieces/m2)) and 3 cages (D1, box; D2, mesh cage; D3, prefabricated multitier trays). In addition, 3 stocking densities (T1, 50 pieces/m ; T2, 100 pieces/m; T3, 200 pieces/m) were evaluated in the prefabricated multitier trays. We found that, in the nursery experiment, 4-mo-old tropical abalone juveniles reared for 90 d showed no significant differences in growth (shell length and body weight) and survival rates among the 3 nursery cages used (Tukey's post hoc test, P > 0.05). Feed conversion ratio, however, was lowest for the high-density treatment T1D3 (7.8 ± 0.76) and was significantly different from the low density treatment T1D1 (11.32 ± 1.2) and intermediate density treatment T1D2 (12.39 ± 1.12; t-test, P > 0.05). Conversely, at higher densities (T2), the same trend applied with abalone reared in multitier basket systems (T2D3), having the highest growth rates and survival rates (29.3 ± 0.07 mm average shell length (ASL) and 5.16 ± 0.52 g average body weight (ABW)), followed closely by those reared in mesh cages (T2D2) and boxes (T2D1). Feed conversion ratio was also lowest for T2D3 (7.56 ± 0.79) and was significantly lower than T2D1 and T2D2. Between treatments, however, abalone reared at lower densities (T1) had significantly higher growth and survival than those reared at higher densities (T2), regardless of the nursery cage used, indicating an inverse relationship between stocking density, growth, and survival. For the grow-out study, tropical abalone reared in multitier trays at low densities (T1) attained the highest growth in shell length and body weight (49.7 ± 0.11 mm ASL and 29.8 ± 2.6 g ABW, respectively) at 180 d of culture, which was significantly greater than those reared in the high-density treatment (T3) with significantly smaller shell length and body weight (43.8 ± 0.18 mm ASL and 21.2 ± 2.0 g ABW), but not significantly different than the intermediate density treatment. This trend started from day 60 of culture onward when analyzed using Duncan's multiple range test (P > 0.05). Survival rates were not significantly different among stocking density treatments, nor were feed conversion ratios. We recommend, for nursery rearing of abalone juveniles, using multitier trays (D3) or boxes (D1) at 500 pieces/m2 stocking density to attain a grow-out size of 26–30 mm in shell length in 90 days. A stocking density of 100 pieces/m2 is recommended to grow abalone in multitier trays to attain a cocktail size of 50 mm ASL and 30 g ABW in 180 d with survival rates between 85.6% and 83.1%.

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