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    • Computer-based resources for aquatic animal health 

      Arthur, James Richard (Fish Health Section, Asian Fisheries Society, 2002)
      A brief review of computer-based resources for aquatic animal health is presented, with emphasis on information sources likely to be of interest to scientists, diagnosticians, and university instructors working in the Asian Region. Examples discussed include multimedia websites and CD-ROMs such as the Food and Agriculture Organization s Aquatic Animal Pathogen and Quarantine Information System (AAPQIS), diagnostics programs such as Alday de Graindorge and Flegel s Diagnosis of Shrimp Diseases, and Fishvet s Fish-Vet and Aquarium Hobby-Vet ; fish histology and necropsy software such as the University of Maryland, Aquatic Pathobiology Center s FishGuts and the Akvapatologisk Laboratorium s AquaPath©: Histology Atlas of Salmonids; more broadly based fisheries software such as ICLARM s FishBase; and a wide range of websites, homepages and discussion groups.
    • Studies on the efficacy of Sarafin® (sarafloxacin hydrochloride) on vibrios associated with vibriosis in black tiger shrimp (Penaeus monodon) 

      Pakingking, Rolando V., Jr. ORCID; Lacierda, Erlinda; Torres, James L. (Fish Health Section, Asian Fisheries Society, 2002)
      In vitro activity of Sarafin® (sarafloxacin hydrochloride) was determined against 7 luminous Vibrio harveyi isolates and 3 non-luminous Vibrio species (V. parahaemolyticus, V. alginolyticus and Vibrio species) isolated from diseased shrimp (Penaeus monodon) and rearing water, a strain of V. anguillarum from diseased marine fish, 1 strain each of V. alginolyticus, V. vulnificus, and V. mimicus from diseased grouper (Epinephelus coioides), and V. alginolyticus from diseased seabass (Lates calcarifer). Bacterial susceptibility was expressed as minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The MIC and MBC values obtained for all V. harveyi isolates ranged from <0.3 to 1.25 µg/ml. For the non-luminous Vibrio species, MIC and MBC values ranged from <0.08 to 1.25 µg/ml. In vivo tolerance levels (24 h static bioassay) of larval and postlarval stages of P. monodon for Sarafin® were <10 µg/ml for nauplii, mysis, and postlarvae and ≤1 µg/ml for zoeae. Morphological deformities in the carapace, rostrum, and setae were noted among larvae exposed to >50 µg/ml Sarafin®. These results indicate that Sarafin® is a potential candidate as a chemotherapeutic agent against luminous vibriosis in P. monodon.
    • Swollen hindgut syndrome (SHG) in hatchery-reared Penaeus monodon postlarvae 

      Lavilla-Pitogo, Celia R.; Paner, Milagros G.; Traviña, Remia D. (Fish Health Section, Asian Fisheries Society, 2002)
      In the course of routine microscopic analysis of hatchery-reared Penaeus monodon postlarvae, several batches were found with hindgut abnormalities not previously described in shrimp postlarvae. The abnormality was named swollen hindgut syndrome (SHG) because it affected mainly the hindgut. Postlarvae with SHG showed enlargement and distention of the hindgut folds and its junction with the midgut, although in some cases swelling also occurred in the midgut of the sixth abdominal segment. Over a five-year period, the yearly prevalence of SHG ranged from 6 to 13% of all batches examined. No seasonal pattern was observed as SHG occurred year-round. Despite the numerous samples obtained, SHG has not been associated with specific predisposing factors in the hatchery. The abnormality caused cessation of the rhythmic movements of the hindgut-midgut junction resulting to failure of affected postlarvae to excrete fecal pellets. Swollen hindgut syndrome, although reversible to some extent, caused mortality and significant size variation within batches of postlarvae resulting in their unsuitability for stocking in grow-out farms.