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Detection of shrimp viral pathogens with a simple dot-blot enzyme immunoassay protocol

dc.contributor.authorNadala, Jr., Elpidio Cesar B.
dc.contributor.authorYou, Zerong
dc.contributor.authorLoh, Philip C.
dc.contributor.editorLavilla-Pitogo, Celia R.
dc.contributor.editorCruz-Lacierda, Erlinda R.
dc.date.accessioned2021-10-15T07:47:18Z
dc.date.available2021-10-15T07:47:18Z
dc.date.issued2002
dc.identifier.citationNadala, Jr., E. C. B., You, Z., & Loh, P. C. (2002). Detection of shrimp viral pathogens with a simple dot-blot enzyme immunoassay protocol. In C. R. Lavilla-Pitogo & E. R. Cruz-Lacierda (Eds.), Diseases in Asian aquaculture IV: Proceedings of the Fourth Symposium on Diseases in Asian Aquaculture, 22-26 November 1999, Cebu City, Philippines (pp. 37-44). Fish Health Section, Asian Fisheries Society.en
dc.identifier.isbn9718020160
dc.identifier.urihttp://hdl.handle.net/10862/6194
dc.description.abstractDot-blot nitrocellulose enzyme immunoassays (DB-NC-EIA) were developed for the detection of two shrimp viral pathogens, white-spot virus (WSV), and yellow-head virus (YHV). The assays utilized HRP-conjugated virus-specific polyclonal antibodies to detect virus antigen present in gill homogenates of infected shrimp spotted onto nitrocellulose membrane. The DB-NC-EIA was highly specific and sensitive enough to detect the presence of viral proteins before the appearance of overt symptoms. The assays are one of the simplest and most rapid detection methods available for WSV and YHV.en
dc.publisherFish Health Section, Asian Fisheries Societyen
dc.subjectprawns and shrimpsen
dc.subjectviral proteinsen
dc.titleDetection of shrimp viral pathogens with a simple dot-blot enzyme immunoassay protocolen
dc.typeConference paperen
dc.citation.spage37en
dc.citation.epage44en
dc.citation.conferenceTitleDiseases in Asian aquaculture IV: Proceedings of the Fourth Symposium on Diseases in Asian Aquaculture, 22-26 November 1999, Cebu City, Philippinesen
dc.subject.asfaanimal diseasesen
dc.subject.asfadiseasesen
dc.subject.asfapathogensen
dc.subject.asfaenzymesen
dc.subject.asfaviral diseasesen
dc.subject.asfavirusesen


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