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dc.contributor.authorChanphong, Jitkasem
dc.contributor.authorChinabut, Supranee
dc.contributor.authorPolchana, Jaree
dc.contributor.authorThompson, Kim D.
dc.contributor.authorAdams, Alexandra
dc.contributor.editorLavilla-Pitogo, Celia R.
dc.contributor.editorCruz-Lacierda, Erlinda R.
dc.date.accessioned2021-11-18T01:02:18Z
dc.date.available2021-11-18T01:02:18Z
dc.date.issued2002
dc.identifier.citationChanphong, J., Chinabut, S., Polchana, J., Thompson, K. D., & Adams, A. (2002). Production and characterization of a monoclonal antibody against Aeromonas hydrophila. In C. R. Lavilla-Pitogo & E. R. Cruz-Lacierda (Eds.), Diseases in Asian aquaculture IV: Proceedings of the Fourth Symposium on Diseases in Asian Aquaculture, 22-26 November 1999, Cebu City, Philippines (pp. 355-360). Fish Health Section, Asian Fisheries Society.en
dc.identifier.isbn9718020160
dc.identifier.urihttp://hdl.handle.net/10862/6227
dc.description.abstractA monoclonal antibody (MAb), 8D10, was produced against Aeromonas hydrophila strain 94030 isolated from giant gourami (Osphronemus gourami). This was classified as an IgG2a immunoglobulin by ELISA, and recognised a 41 kDa band on whole cell preparations of the A. hydrophila by Western blotting. The reactivity of the MAb against twenty-five different A. hydrophila isolates was examined by an indirect ELISA and Western blotting. Twelve other bacterial species were also tested by the indirect ELISA to examine the specificity of the MAb. One of the A. hydrophila isolate (strain 95014) gave a strong reaction (89.9%) with the MAb in the ELISA and also recognised the 41 kDa band. Sixteen other strains were weak positive in the ELISA, and eleven of these strains were weakly positive by Western blotting. No cross-reaction with the non-A. hydrophila isolates occurred.en
dc.publisherFish Health Section, Asian Fisheries Societyen
dc.subjectAeromonas hydrophilaen
dc.subjectimmunoglobulinsen
dc.subjecthybridomasen
dc.titleProduction and characterization of a monoclonal antibody against Aeromonas hydrophilaen
dc.typeConference paperen
dc.citation.spage355en
dc.citation.epage360en
dc.citation.conferenceTitleDiseases in Asian aquaculture IV: Proceedings of the Fourth Symposium on Diseases in Asian Aquaculture, 22-26 November 1999, Cebu City, Philippinesen
dc.subject.asfamonoclonal antibodiesen
dc.subject.asfafreshwater fishesen
dc.subject.asfaantigensen
dc.subject.asfaimmunizationen
dc.subject.scientificNameOsphronemus gouramien


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