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dc.contributor.authorDe Mesa, Czarina Anne
dc.contributor.authorMendoza, Remilyn
dc.contributor.authorAmar, Edgar
dc.contributor.authorde la Peña, Leobert D.
dc.contributor.authorSaloma, Cynthia
dc.coverage.spatialNegrosen
dc.date.accessioned2022-07-04T03:51:50Z
dc.date.available2022-07-04T03:51:50Z
dc.date.issued2021-02-27
dc.identifier.citationDe Mesa, C. A. E., Mendoza, R. M., Amar, E. C., de la Peña, L. D., & Saloma, C. P. (2021). Identification of a chromosomally-encoded sucrose operon-like gene cluster in Vibrio parahaemolyticus strain PH05 isolated from Negros Island, Philippines [Preprint]. bioRxiv. https://doi.org/10.1101/2021.02.27.433172en
dc.identifier.urihttp://hdl.handle.net/10862/6337
dc.description.abstractThe ability of bacteria to metabolize a wide variety of carbon sources has been known to aid in their ability for efficient colonization. Vibrio parahaemolyticus, a known aquatic pathogen has been reported to have the ability to metabolize a number of carbohydrates including D-glucose, D-galactose, L-arabinose, D-mannose, and D-ribose to name a few. Classical isolation of V. parahaemolyticus from other members of the family Vibrionaceae relies on its carbon utilization pattern. Conventionally, V. parahaemolyticus lacks the ability to utilize sucrose and this has been the basis for its isolation using the Thiosulfate-citrate-bile salts-sucrose (TCBS) agar. Reports of V. parahaemolyticus having the ability to utilize sucrose have been presented yet there is paucity of information and detailed study on this phenotype. In this study, we report the V. parahaemolyticus strain PH05 that has the ability to metabolize sucrose. Phenotypic and genotypic characterization of this V. parahaemolyticus strain isolated from Negros Island, Philippines, revealed that V. parahaemolyticus strain PH05 is atypical appearing yellow on TCBS agar plates. It is capable of utilizing sucrose, unlike the majority of V. parahaemolyticus isolates. Genome analyses of this strain revealed the presence of a chromosomally encoded sucrose operon-like gene cluster encoded in chromosome 2 with the following sucrose-utilization associated genes: scrY, ccpA, treP, scrK, and scrB genes coding for sucrose porin, catabolite control protein A, PTS System sucrose-specific EIIBC component, fructokinase, and sucrose-6-phosphate hydrolase. The mode of transmission of these genes to V. parahaemolyticus strain PH05 is still unknown. However, the presence of insertion sequences (IS) and phage elements in the same chromosome suggests horizontal gene transfer events. Taken together, our results point to the possibility that acquired sucrose utilization genes may contribute to the fitness of V. parahaemolyticus strain PH05 in the environment.en
dc.description.sponsorshipThis work was supported by a Department of Science and Technology, Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (DOST-PCAARRD) grant to CPS, EAA, LDD, and a DOST Science Education Institute fellowship to CADM and RMM.en
dc.language.isoenen
dc.publisherCold Spring Harbor Laboratoryen
dc.relation.urihttps://www.biorxiv.org/content/10.1101/2021.02.27.433172v1.full.pdfen
dc.subjectVibrio parahaemolyticusen
dc.subjectPhilippinesen
dc.titleIdentification of a chromosomally-encoded sucrose operon-like gene cluster in Vibrio parahaemolyticus strain PH05 isolated from Negros Island, Philippinesen
dc.typePreprinten
dc.identifier.doi10.1101/2021.02.27.433172
dc.citation.journalTitlebioRxiven
dc.subject.asfapathogensen
dc.subject.asfapathogenic bacteriaen
dc.subject.asfagenomesen
dc.subject.asfachromosomesen
dc.subject.asfaphenotypesen
dc.subject.scientificNameVibrio parahaemolyticusen
dc.subject.scientificNameVibrioen
local.subjectVibrio parahaemolyticusen
local.subjectatypicalen
local.subjectsucroseen
local.subjectgene clusteren
local.subjectoperonen


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  • Journal Articles [1222]
    These papers were contributed by Department staff to various national and international journals.

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