Production, feeding and storage of Tetraselmis tetrathele paste by electrolytic flocculation
View/
Date
2023-02Page views
1,102ASFA keyword
AGROVOC keyword
Taxonomic term
Metadata
Show full item recordShare
Abstract
An innovative microalgae harvesting technique using electroflocculation was carried out at different voltage treatments (4, 7, and 9 V) using a fabricated voltage regulator and compared with harvesting by electroflocculation at 12 V using a car battery to efficiently electroflocculate Tetraselmis tetrathele. This study was conducted to determine the ideal voltage settings that could improve the quality of harvested paste in terms of nutritional value and metal contamination for its later use in aquaculture. The use of 7 V achieved low Pb content producing the best harvest biomass (1.4 kg, 87 min) which is comparable to the harvest biomass (1.7 kg, 68 min) using a 12 V car battery. Storage of T. tetrathele paste is best done in a chiller (2 ± 1 °C) rather than a freezer (-20 ± -4 °C) for ease in reactivation and maintaining higher cell viability for later use as a starter in aquaculture. Storage in a chiller allows T. tetrathele paste to be resuspended after six months and used as a starter culture.
Keywords
Electro-flocculation Microalgal paste Microalgae harvesting Pb content Tetraselmis tetratheleSuggested Citation
Villa-Franco, A., Teves, C. M. L., Dato-on, K. L. G., de Jesus-Ayson, E. G. T., Usero, R., & de la Peña, M. R. (2023). Production, feeding and storage of Tetraselmis tetrathele paste by electrolytic flocculation. Journal of Applied Phycology , 35(1), 85-90. https://doi.org/10.1007/s10811-022-02887-9
Type
ArticleISSN
0921-8971; 1573-5176Collections
- Journal Articles [1266]
Related items
Showing items related by title, author, creator and subject.
-
Use of rotifers-fed microalgal paste in the seed production of mangrove crab Scylla serrata in the Philippines
Huervana, Joana Joy
; ; Villa-Franco, Annie (Springer, 2022-09-09)
Despite the progress in the production of artificial diets for marine larvae, feeding during the early life stages of most aquaculture species still relies on live feeds such as rotifers. Advanced rotifer culture techniques are also available, however, in the Philippines, majority use batch cultures with fresh microalgae. These microalgae are prone to collapse resulting to inadequate supply of food for the rotifers that are fed to the larvae. Mangrove crab is one of the economically important aquaculture species in the country where mass seed production requires sufficient and steady supply of rotifers. Thus, here we examined the possibility of substituting fresh culture of green microalgae Tetraselmis tetrathele (FA) with flocculated T. tetrathele paste (FP) and commercially available Tetraselmis sp. (CP) as feed for rotifers (Brachionus rotundiformis) in the seed production of mangrove crab. Rearing of larvae was done until crab instar 1 (DOC 24) where growth and survival were taken to evaluate the viability of rotifers-fed microalgal paste. It was observed that the growth index (FA–6.93 ± 0.07; FP–6.91 ± 0.07; CP–6.96 ± 0.05) was similar for all treatments (P > 0.05) at termination. Comparable survival rates were noted for FA and FP at 1.08 ± 0.59% and 3.21 ± 2.09%, respectively (P > 0.05) while a significantly higher survival (P < 0.05) was recorded for CP at 7.73 ± 1.90%. These findings suggest that the algal paste tested are potential alternatives to fresh microalgae and this technique could benefit hatchery operators who lack the capital for setting up the facility and technical skills to maintain microalgae production. -
Acute toxicity of water-accommodated fraction and chemically enhanced WAF of bunker C oil and dispersant to a microalga Tetraselmis tetrathele
Santander-Avancena, Sheryll; Sadaba, Resurreccion ; ; ; (Springer Verlag, 2016)
This study assessed the toxicity of water-accommodated fraction (WAF) and chemically enhanced WAF (CEWAF) of bunker C oil and dispersant (DISP) to a microalga, Tetraselmis tetrathele. The 72-h median effective concentration (72-h EC50) of CEWAF and DISP were determined at 3.30 % and 2.40 %, respectively. The no observed effect concentration (NOEC) of CEWAF to T. tetrathele was at 2.0 % and lowest observed effect concentration (LOEC) was at 3.0 % while NOEC and LOEC of DISP to T. tetrathele were determined at 1.0 % and 2.0 %, respectively. The addition of dispersant to oil increased the amount of total PAH present in the CEWAF test solutions. DISP alone was highly toxic, and the toxicity of CEWAF was primarily caused by the presence of dispersant. -
Live food: A lesser known essential
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2003)This article is a short discussion of the requirements for live food production in aquaculture and a brief presentation of the processes involved.
