Effect of temperature and salinity on the hatching of eggs and larval development of sugpo, Penaeus monodon.
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Incubation of Penaeus monodon eggs and rearing of different larval stages were undertaken at nine temperature-salinity combinations. The eggs, nauplii, zoea and mysis from one spawner kept as stock culture at ambient temperatures of 26-30°C and salinity of 32-33 ppt were exposed to temperature levels of 23, 28 and 33°C and salinity levels of 23, 28 and 33 ppt. Eggs and nauplii survived the sudden change of temperature and salinity (from ambient to experimental) but the zoea and mysis did not. However, salinities of 23 and 28 ppt in combination with any of the temperature levels produced weak larvae. Highest mean hatching rate was obtained at the temperature-salinity combination of 23°C-33 ppt, followed by 28°C-33 ppt and 33°C-33 ppt. Incubation periods for these treatments were 22, 16 and 14 hr, respectively. Survival rate of nauplius (taken from stock cultures) to first zoeal stage was highest at 28°C-33 ppt, followed by 33°C-33 ppt and 23°C-33 ppt with molting time of 50, 45 and 75 hr, respectively. The nauplii exposed to 33°C-33 ppt molted to zoea stage within 38 to 40 hr but later died. Those exposed to 23°C-33 ppt and 28°C-33 ppt reached zoea stage within 57 to 60 hr and 48 to 50 hr, respectively. Similarly, the nauplii taken from the stock cultures and reared until postlarval stage (P1) under experimental conditions completed the zoea and mysis stages in 9 to 11 days at 28°C C-33 ppt, 7 to 9 days at 33°C-33 ppt, and 13 to 15 days at 23°C-33 ppt. Statistical analysis showed that salinity had highly significant effect on rates of hatching of eggs and survival from nauplius to first zoeal stage but not temperature although the latter had an apparent effect. However, both factors affected time of hatching of eggs and time of molting from nauplius to zoea. Interaction effect was significant only on rate and time of hatching. Different sources (spawners) of eggs and nauplii did not have significant effect on time of hatching and molting from nauplius to zoea, but significantly affected the hatching rate of eggs and survival rate of nauplii to zoea stage.
Reyes, E.P. (1985). Effect of temperature and salinity on the hatching of eggs and larval development of sugpo, Penaeus monodon. (Abstract only). In Taki Y., Primavera J.H. and Llobrera J.A. (Eds.). Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps, 4-7 December 1984, Iloilo City, Philippines (pp. 177-178). Iloilo City, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center. http://hdl.handle.net/10862/928
PublisherAquaculture Department, Southeast Asian Fisheries Development Center
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Conference paperAC Gonzal, CB Santiago & WB Afuang - In Conservation and Ecological Management of Philippine Lakes in Relation to Fisheries and Aquaculture: Proceedings … Seminar-Workshop held on October 21-23, 1997, INNOTECH, Commonwealth Ave., Diliman, Quezon City, Philippines, 2001 - Aquaculture Department, Southeast Asian Fisheries Development Center; Philippine Council for Aquatic and Marine Research and Development (PCAMRD), Department of Science and Technology; Bureau of Fisheries and Aquatic ResourcesThe backflow of Pasig River into Laguna de Bay was closely monitored beginning April 28, 1997 based on the expected high tide in Manila Bay and the average low lake level of 2.5 m in Station W (N 14°27.7'; E 121°08.5') located at the west lobe of the lake. Saltwater intrusion was first detected during of the highest high tide (i.e., 1.5 m at 1430 h) on April 29. Water from Pasig River flowing into the lake was black and had a characteristic odor of hydrogen sulfide. With the light house (or 'Parola') at the mouth of Pasig River as the reference point, movement of saltwater in the lake was monitored and the area affected estimated with the use of GPS 38 Personal Navigator®. Up to mid May (Week 2), the movement of saltwater into Laguna de Bay was hampered by the intermittent calm weather conditions and moderately strong northeasterly wind ('hanging amihan'). When the wind direction shifted and the southwesterly wind ('hanging habagat') became strong on Week 3 (May 18 to 20), strong water movement and fast diffusion of saltwater into the other parts of the west lobe of the lake were observed. During this period of rapid change in the lake (May 21-24), a high frequency monitoring was conducted in Station W. Fluctuations in chloride ion concentration, conductivity and total dissolved solids, Secchi disc reading, turbidity, dissolved oxygen, and other parameters were noted in the station. Heavy rainfall in the area on May 24-26 and run-offs from the watershed and overflow from the river tributaries increased the lake level. The elevation of the water level resulted in draining out of the lake water into Pasig River to Manila Bay and this practically ended the year's saltwater intrusion into Laguna de Bay. Movement of saltwater reached almost the whole area of the west and central lobes of the lake on the first week of June (week 5) as evidenced by the clearing of water in those areas. It was estimated that clearing of the entire lake because of saltwater movement takes about 2-3 months.
A preliminary study on the effect of hypoxia on carotenoid metabolism in black tiger shrimp Penaeus monodon Fabricius FI Heralde, M Leaño, A Reyes & R Coloso - 1996 - Aquaculture Department, Southeast Asian Fisheries Development CenterThe pigmentation of black tiger shrimp Penaeus monodon is due to astaxanthin, astaxanthin monoester, and astaxanthin diester. These carotenoids are biosynthesized from beta-carotene or zeaxanthin. Biosynthesis is postulated to be mediated by the enzymes C3 and C4 monooxygenase and caroten-4-ol dehydrogenase. Blue-shrimp syndrome, characterized by low total astaxanthin levels in shrimp epidermis, is associated with nutritional deficiency for carotenoids but other factors such as high organic matter, hypoxia, high density, and high pH may also be involved. In this study, the effect of hypoxia on carotenoid metabolism in P. monodon was investigated with respect to retention and transformation of carotenoids and the activity of the enzymes C3 and C4 monooxygenase and caroten-4-ol dehydrogenase.Two tanks were prepared each containing 25 pieces of shrimp fed a control diet (not containing any carotenoid) for two weeks. Shrimps in one tank (Treatment 1) were given beta-carotene supplemented diet (3.8 ppm) in the next two weeks while those in the other (treatment 2) were fed the control diet. Two days after introduction of the test diets, both treatments were subjected to hypoxia challenge (dissolved oxygen, 2.5-2.7 mg/L) and the carotenoid content and profile of feces monitored for succeeding days. Assay of enzyme activity was performed five days after hypoxia challenge. Results showed a ten-fold increase in fecal total carotenoid content of shrimps in treatment 2 (122.2 ppm) compared with that in treatment 1 (12.4 ppm) on hypoxia challenge and restores to basal level (3.9-4.1 ppm) after three days. The carotenoids found in the feces of shrimps in treatment 2 were astaxanthin, astaxanthin monoester, astaxanthin diesters, and an unidentified carotenoid which was also found in the muscle of stressed shrimp. Using beta-carotene as substrate, conversion to astaxanthin was not observed in the hepatopancreas homogenates of shrimps in both treatments. Instead, a slow conversion of astaxanthin to betacarotene (i.e., a reverse reaction) was noted in treatment 2 exhibiting twice the activity in treatment 1. Radiolabelling studies using 14C-labelled astaxanthin as substrate confirmed the existence of this enzyme-mediated reductive pathway from astaxanthin which occurs at a very slow rate. This study demonstrated the carotenoid-depleting effect of hypoxia on shrimp, both through enhanced fecal release and moderately elevated reductive pathway from astaxanthin. This effect suggests a possible mechanism by which blue-shrimp syndrome may develop.
Effect of various levels of squid protein on growth and some biochemical parameters of Penaeus japonicus juveniles. LC Suarez, J Guillaume & AV Wormhoudt - In Y Taki, JH Primavera & JA Llobrera (Eds.), Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps, 4-7 December 1984, Iloilo City, Philippines, 1985 - Aquaculture Department, Southeast Asian Fisheries Development CenterAn unknown growth factor previously suspected in squid meal was found in the protein fraction of squid (Loligo vulgaris). It is clearly different from hydro-alcohol-soluble feed attractants that are also present in squid meal. This squid protein fraction (SPF) improves the growth of Penaeus japonicus juveniles when added either in a semi-purified or in a more complex mixed diet. This growth-promoting effect does not seem to be related to the amino acid composition of SPF. In order to obtain more information on its action, several levels (1.5 to 16.0%) of SPF were added to a mixed diet. The diets were isoproteic (59% D.M.), isolipidic (8.5% D.M.), supplemented with vitamins, cholesterol, glucosamine, etc. They were fed as wet pellets to 3 replicates of 15 shrimp; blue mussel was used as the control. The growth of shrimp increased with the SPF level and attained a plateau above 6%. Body weight was significantly higher than that of the control group at this level. RNA content and RNA:DNA ratio increased with the SPF level indicating that growth was improved more by hypertrophy than by hyperplasy of the cells. The hepatosomatic ratio remained unchanged. The assay of two digestive enzymes, proteases and amylases, showed no clear effect of SPF on protease or amylase activities. More experiments are needed to explain the effect of the unknown growth factor of SPF.