Luminescent vibrios associated with mortality in pond-cultured shrimp penaeus monodon in the Philippines: Species composition
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Heavy mortalities due to luminescent vibriosis have been observed among pond-cultured Penaeus monodon shrimp in the Philippines. The species composition of luminescent Vibrio associated with mortalities was determined. A total of 189 luminescent bacteria isolated from the hepatopancreas of affected shrimps, rearing water and seawater from different shrimp farms in 11 provinces of the Philippines were examined for their morphological, physiological and biochemical characteristics. Results revealed a varied composition of Vibrio species. The most dominant luminescent Vibrio species was V. harveyi (65.5%) followed by V. logei (7%), Photobacterium sp. (6%) and V. orientalis (1%). Some isolates, based on their characteristics, were identified as V. campbellii (16%), V. mediterranei (3%), V. fluvialis (0.5%), V. cholerae (0.5%) and V. splendidus II (0.5%), which are known as non-luminescent Vibrios. V. harveyi is thought to be the major etiological agent associated with the luminescent vibriosis in pond-cultured P. monodon and its pathogenicity was confirmed through intramuscular injection to shrimp. Experimental infection showed that V. campbellii was also pathogenic to P. monodon.
Citationde la Peña, L. D., Lavilla-Pitogo, C. R., & Paner, M. G. (2001). Luminescent vibrios associated with mortality in pond-cultured shrimp penaeus monodon in the Philippines: Species composition.
PublisherJapanese Society of Fish Pathology
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Studies on the efficacy of Sarafin® (sarafloxacin hydrochloride) on vibrios associated with vibriosis in black tiger shrimp (Penaeus monodon) RV Pakingking Jr. - In CR Lavilla-Pitogo & ER Cruz-Lacierda (Eds.), Diseases in Asian Aquaculture IV. Proceedings of the Fourth Symposium on Diseases in Asian Aquaculture, 22-26 November 1999, Cebu City, Philippines, 2002 - Fish Health Section, Asian Fisheries SocietyIn vitro activity of Sarafin® (sarafloxacin hydrochloride) was determind againts 7 luminous Vibrio harveyi isolates and 3 non-luminous Vibrio species (V. parahaemolyticus, V. alginlyticus and Vibrio species) isolated from diseased marine shrimp (Penaeus monodon) and rearing water, a strain of V. anguillarum from diseased marine fish, I strain each of V. alginolyticus, V. vulnificus, and V. mimicus from diseased grouper (Epinephelus coioides), and V. alginolyticus from diseased seabass (Lates calcarifer). Bacterial susceptibility was expressed as minimum inhabitory concentration (MIC) and minimum bactericidal concentration (MBC). The MIC and MBC values obtained for all V. harveyi isolates ranged from <0.3 to 1.25µg/ml. For the non-luminous vibrio species, MIC and MBC values ranged from <0.08 to 1.25µg/ml. In vivo tolerance level (24 h static bioassay) of larval and postlarval stage of P. monodon for Sarafin® were ≤10 µg/ml for nauplii, mysis, and postlarvae and ≤1 µg/ml for zoeae. Morphological deformities in the carapace, rostrum, and setae were noted among larvae exposed to ≤50 µg/m Sarafin®. These results indicate that Sarafin® is a potential candidate as a chemotherapeutic agent againts luminous vibriosis in P. monodon.
ArticleFrom January 1990 to June 1993, 59% of the total juvenile to adult shrimp (Penaeus monodon) submitted for diagnosis at the Fish Health Section of the Aquaculture Department, Southeast Asian Fisheries Development Center, Tigbauan, Iloilo, Philippines were diagnosed to have red disease syndrome. Red disease syndrome is characterized by the reddening of the shrimp body. The aetiology of the disease is unknown. This paper reports for the first time the isolation of four Vibrio phenotypes, namely, Vibrio harveyi, V. parahaemolyticus, V. fluuialis and Vibrio sp. from shrimps with red disease. Pathogenicity test shows that injection with V. parahaemolyticus and V. harveyi can produce the characteristic red discoloration in healthy shrimp.
ArticleCR Lavilla-Pitogo, AR Castillo & MC de la Cruz -
Journal of Applied Ichthyology, 1992 - Blackwell PublishingVibrio sp., was consistently isolated from grouper, Epinephelus suillus, with bacterial infection. Fingerlings, which were challenged with the bacterium by injection, were highly susceptible. Immersion challenge resulted in 100% mortality within 48 hrs in fish subjected to combination of injury and exposure to the bacterium. Mortality in uninjured fish was observed in the long bath subgroup, but not in the short bath subgroup. These results are correlated with the present practices in the grouper fingerling industry in the Philippines.