Growth and body composition of juvenile mud crab, Scylla serrata, fed different dietary protein and lipid levels and protein to energy ratios.
MetadataShow full item record
Cited times in Scopus
The effect of different dietary protein and lipid levels, and protein to energy (P/E) ratios on growth and body composition of the mud crab, Scylla serrata, was evaluated. Six practical test diets were formulated to contain three protein levels (32%, 40% and 48%) at two lipid levels (6% and 12%), each with P/E ratios ranging from 20.5 to 31.1 mg protein/kJ. Individual crabs were stocked in 36 units of 60-l tanks and maintained on a 40% protein diet until each molted (M0). Newly molted crabs were weighed and fed the test diets until termination at 30 days from the third molt (M3+30). Crabs were monitored daily and body weight (BW) taken after each molt, at intermolt and at termination. Average initial BW (11.18±0.66 g) was taken at 18 days after M0. Carapace width (CW) at M3+30 and of the exuviae (at molt 1, 2, and 3 or M1, M2 and M3), weight of exuviae (M1 to M3), feed conversion ratio or FCR, duration of intermolt, and total number of days of feeding test diets (M0 to M3+30) were determined. At the end of the study, crabs were freeze-dried for analysis of nutrients in the flesh, exoskeleton and fat body. The FCR (3.21–4.21), intermolt duration and total number of days of feeding test diets (111.3–131.2 days) were not affected by dietary treatments (P>0.05). Analysis of covariance was used with CW at M1 and BW at M0+18 as covariates. CW in the 40% protein with 6% lipid or 40/6 diet (P/E ratio, 27.5 mg protein/kJ) did not increase when lipid was increased to 12% (40/12), and it was significantly wider than crabs fed the 48/6 and 48/12 diets (P/E ratios, 31.1 and 27.2 mg protein/kJ). CW and BW did not differ in the 40% and 32% protein diets and were not affected by dietary lipid level at every level of protein. Ca in the exoskeleton was lowest in the 32/6 diet, while exuviae weight was about one-fourth of BW. Crude fat in the lipid deposit of crabs fed 48% protein diets were low. Results showed that the mud crab, S. serrata, grow well when fed diets containing 32–40% dietary protein with either 6% or 12% lipid at dietary energy ranging from 14.7–17.6 MJ/kg.
CitationCatacutan, M. R. (2002). Growth and body composition of juvenile mud crab, Scylla serrata, fed different dietary protein and lipid levels and protein to energy ratios.
Showing items related by title, author, creator and subject.
Evaluation of dietary freeze-dried Chaetoceros calcitrans supplementation to control Vibrio harveyi infection on Penaeus monodon juvenile Effects of supplementation of diets with freeze-dried Chaetoceros calcitrans to control Vibrio harveyi infection are evaluated through immune responses, and disease resistance of juvenile Penaeus monodon. Total lipid and fatty acid profile of Chaetoceros calcitrans is also analyzed. A challenge infection with 107 cfu/mL concentration of Vibrio harveyi is intramuscularly injected to juvenile Penaeus monodon after 45 days of feeding of diets supplemented with 15 g/kg and 30 g/kg dried Chaetoceros calcitrans. The use of dried Chaetoceros calcitrans is compared with that of ß-1,3 glucan Curdlan, a commercial immune enhancer. Incorporation of 30 g/kg Chaetoceros calcitrans in the diet enhances the immune system of shrimp as effected by high prophenoloxidase activity and plasma protein concentration and is better compared to the commercially available Curdlan. Chaetoceros calcitrans also contains polyunsaturated fatty acids (PUFAs) such as linolenic acid and eicosapentanoic acid (EPA) which are responsible for its antibacterial action against Vibrio harveyi. All these biological activities of Chaetoceros calcitrans add up to increase resistance of the juvenile Penaeus monodon to vibriosis as shown by its high survival rate from the challenge infection with Vibrio harveyi. Therefore, it is worthwhile to use Chaetoceros calcitrans as supplementary feed. Its effect in increasing the immune competence coupled with its antibacterial action, make the shrimp resistant to luminous vibriosis that continues to affect the industry, thereby augmenting aquaculture production.
Molecular cloning and localization of GABAA receptor-associated protein in the rotifer Brachionus plicatilis HS Marcial, K Suga, S Kinoshita, G Kaneko, A Hagiwara & S Watabe -
International Review of Hydrobiology, 2014 - Wiley-VCH Verlagγ-Aminobutyric acid receptor type A-associated protein (GABARAP) and its homologs constitute a protein family found in many eukaryotes from yeast to human, and are known to be involved in intracellular membrane trafficking of GABAA receptors and autophagy. In this study, we cloned cDNA-encoding GABARAP from the monogonont rotifer Brachionus plicatilis and examined for its tissue distribution at the protein level in neonates, males and females. Using reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE) techniques, we showed that like other GABARAPs, rotifer GABARAP was also composed of 117 amino acids and highly homologous to vertebrate GABARAP2 ortholog (74–76% identity). GABARAP was demonstrated with its specific antibody to be ubiquitously distributed, irrespective of neonates, males, and females, in the coronal area that covers brain and contains most mechano- and chemoreceptors. Rotifer GABARAP was also expressed in the mature eggs but not in immature eggs. Double immunostaining with mammalian anti-GABA γ receptor antibody showed that rotifer GABARAP co-localized with GABA receptor, suggesting the association of the two proteins. The presence of GABARAP in rotifer implies that it is highly conserved during evolution, and plays important roles in various biological processes.
Conference paperMR Catacutan - In MR Catacutan, RM Coloso & BO Acosta (Eds.), Development and Use of Alternative Dietary Ingredients or Fish Meal Substitutes in Aquaculture Feed Formulation … Ingredients or Fish Meal Substitutes in Aquaculture Feed Formulation, 9-11 December 2014, Nay Pyi Taw, Myanmar, 2015 - Aquaculture Department, Southeast Asian Fisheries Development CenterCultured marine aquatic species are predominantly carnivorous. Major species in the region are seabass, grouper, snapper, tiger shrimp, mangrove crab and abalone. These species, except for abalone, require a high level of dietary protein mostly supplied by marine sources such as fish meal. Global production of marine fish and marine shrimps showed a 3-4 fold increase from 1995 to 2010. For the same period, the usage of commercial feed for production of marine fish and shrimps increased while the fish meal portion in the formulation decreased. This is indicative of fish meal being substituted with alternative sources in commercial feed production, and to some extent the substitution of marine oil which particularly improved the FCR for the marine fish production from 2.0 to 1.9 and for marine shrimps from 2.0 to 1.6. Plant products that include cereal grains, legumes and oilseeds have the most potential among the alternative ingredients for use in aquafeed. The use of these resources for high value marine species is limited due to a variety of anti-nutritional substances they contain. Removal of these substances by processing techniques has improved utilization but with added cost. Hence, fish meal is still the primary source of protein for marine carnivores and its substitution with higher amounts of alternative plant proteins may be difficult compared with lower levels of replacements. The Asian region has accounted for the more than 50% of the total global aquaculture production in 2012 with indications of increased utilization of alternative protein sources in commercial feed production. For the major marine species in the region the increasing trend of plant protein usage with the targeted levels of substitution of fish meal with plant protein sources should be sustainable.