Characterization of betanodaviruses in the Philippines
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eafp.orgDate
2008Author
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2,205Subject
catadromous fishes
brain
immunofluorescence
genotypes
fish larvae
nucleotides
marine fish
nervous system
viral diseases
RNA
necrosis
polymerase chain reaction
mariculture
breeding stock
fish culture
Dicentrarchus labrax
Pleuronectiformes
Lates calcarifer
Epinephelus coioides
Philippines, Panay I., Iloilo

brain

immunofluorescence

genotypes

fish larvae

nucleotides

marine fish

nervous system

viral diseases

RNA

necrosis

polymerase chain reaction

mariculture

breeding stock

fish culture

Dicentrarchus labrax

Pleuronectiformes

Lates calcarifer

Epinephelus coioides

Philippines, Panay I., Iloilo
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Abstract
Viral nervous necrosis caused by betanodaviruses is one of the most devastating diseases in cultured marine finfish. In the Philippines, mass mortalities occurred in sea bass, Lates calcarifer larvae and grouper, Epinephelus coioides broodstock. The virus was isolated using SSN-1 fish cell line and confirmed by PCR. Cytopathic effect started to develop in the cell line 2 days post infection (p.i) with tissue filtrates until the cells completely disintegrated and detached from the flask at 5 days p.i. and the viral protein was detected by immunofluorescence. Sequence analysis revealed that VNN isolated from the brain of grouper broodstock and sea bass larvae were 98.6% similar. Sequence analysis between the Philippine isolates and red-spotted grouper nervous necrosis virus (RGNNV) genotype is 96.9% similar as compared to 72.0% and 64.0% similar with the barfin flounder nervous necrosis virus (BFNNV) and tiger puffer nervous necrosis virus (TPNNV) genotypes, respectively. These results confirm that the Philippine isolates belong to RGNNV genotype.
Suggested Citation
de la Peña, L. D., Mori, K., Quinitio, G. F., Chavez, D. S., Toledo, J. D., Suarnaba, V. S., ... & Nakai, T. (2008). Characterization of betanodaviruses in the Philippines. Bulletin of the European Association of Fish Pathologists , 28(6), 230-237. http://hdl.handle.net/10862/2076
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ArticleISSN
0108-0288Collections
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