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Characterization of betanodaviruses in the Philippines

dc.contributor.authorde la Peña, Leobert D.
dc.contributor.authorMori, K.
dc.contributor.authorQuinitio, Gerald F.
dc.contributor.authorChavez, Denny S.
dc.contributor.authorToledo, Joebert D.
dc.contributor.authorSuarnaba, Vonnie S.
dc.contributor.authorMaeno, Y.
dc.contributor.authorKiryu, I.
dc.contributor.authorNakai, T.
dc.date.accessioned2014-05-22T05:40:30Z
dc.date.available2014-05-22T05:40:30Z
dc.date.issued2008
dc.identifier.citationde la Peña, L. D., Mori, K., Quinitio, G. F., Chavez, D. S., Toledo, J. D., Suarnaba, V. S., … Nakai, T. (2008). Characterization of betanodaviruses in the Philippines. Bulletin of the European Association of Fish Pathologists, 28(6), 230-237.en
dc.identifier.issn0108-0288
dc.identifier.urihttp://hdl.handle.net/10862/2076
dc.description.abstractViral nervous necrosis caused by betanodaviruses is one of the most devastating diseases in cultured marine finfish. In the Philippines, mass mortalities occurred in sea bass, Lates calcarifer larvae and grouper, Epinephelus coioides broodstock. The virus was isolated using SSN-1 fish cell line and confirmed by PCR. Cytopathic effect started to develop in the cell line 2 days post infection (p.i) with tissue filtrates until the cells completely disintegrated and detached from the flask at 5 days p.i. and the viral protein was detected by immunofluorescence. Sequence analysis revealed that VNN isolated from the brain of grouper broodstock and sea bass larvae were 98.6% similar. Sequence analysis between the Philippine isolates and red-spotted grouper nervous necrosis virus (RGNNV) genotype is 96.9% similar as compared to 72.0% and 64.0% similar with the barfin flounder nervous necrosis virus (BFNNV) and tiger puffer nervous necrosis virus (TPNNV) genotypes, respectively. These results confirm that the Philippine isolates belong to RGNNV genotype.en
dc.description.sponsorshipThe study was funded by the Government of Japan Trust Fund to SEAFDEC AQD under the study code 8001-T-FD-FH1104. Thanks are due to Y. Inui, K. Nagasawa, K. Okuzawa and H. Ogata, fish disease experts/trust fund co-manager for their guidance and to M. Peniero, R. Traviña, M. Paner and G. Capulos for their technical assistance.en
dc.language.isoenen
dc.publisherEuropean Association of Fish Pathologistsen
dc.relation.urihttps://eafp.org/download/2008-Volume28/Issue%206/28_230.pdf
dc.subjectDicentrarchus labraxen
dc.subjectPleuronectiformesen
dc.subjectLates calcariferen
dc.subjectEpinephelus coioidesen
dc.subjectPhilippines, Panay I., Iloiloen
dc.titleCharacterization of betanodaviruses in the Philippinesen
dc.typeArticleen
dc.citation.volume28
dc.citation.issue6
dc.citation.spage230
dc.citation.epage237
dc.citation.journalTitleBulletin of the European Association of Fish Pathologistsen
dc.subject.asfacatadromous fishesen
dc.subject.asfabrainen
dc.subject.asfaimmunofluorescenceen
dc.subject.asfagenotypesen
dc.subject.asfafish larvaeen
dc.subject.asfanucleotidesen
dc.subject.asfamarine fishen
dc.subject.asfanervous systemen
dc.subject.asfaviral diseasesen
dc.subject.asfaRNAen
dc.subject.asfanecrosisen
dc.subject.asfapolymerase chain reactionen
dc.subject.asfamaricultureen
dc.subject.asfabreeding stocken
dc.subject.asfafish cultureen
dc.subject.scientificNameLates calcariferen


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  • Journal Articles [1229]
    These papers were contributed by Department staff to various national and international journals.

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