Nutritional evaluation of mysids Mesopodopsis orientalis (Crustacea:Mysida) as live food for grouper Epinephelus fuscoguttatus larvae
MetadataShow full item record
Cited times in Scopus
The potential of mysids Mesopodopsis orientalis as live food source for grouper Epinephelus fuscoguttatus larvae was investigated. In comparison with Artemia biomass, a common live food source in larviculture, mysids contained significantly higher levels of protein, total lipid, eicosapentaenoic acid (EPA, C20:5n−3), and docosahexaenoic acid (DHA, C22:6n−3). DHA was not detected in Artemia biomass. Grouper larvae fed mysids from 35 to 55 days after hatching (DAH) had 2-fold and 3-fold higher specific growth rates and survival, respectively, than those fed Artemia biomass. DHA levels increased 6-fold while EPA levels remained constant in mysid-fed grouper larvae. In contrast, DHA and EPA significantly decreased in Artemia biomass-fed grouper larvae. Furthermore, the specific activities of amylase, lipase and protease generally significantly increased (P< 0.05) in both mysid-fed and Artemia biomass-fed grouper larvae from 35 to 55DAH. A marked increase in the specific activity of amylase was seen in mysid-fed than in Artemia biomass-fed grouper larvae. Results of the nutritional evaluation suggest that mysids are superior live food organisms than Artemia biomass for grouper larvae and could significantly improve production of grouper juveniles in the nursery phase.
CitationEusebio, P. S., Coloso, R. M., & Gapasin, R. S. J. (2010). Nutritional evaluation of mysids Mesopodopsis orientalis (Crustacea:Mysida) as live food for grouper Epinephelus fuscoguttatus larvae.
The authors express their appreciation and thanks to Zenith Gaye Orozco for technical assistance, Joseph Biñas for the assistance in statistical analysis of the data and preparation of the manuscript, Gwen Anuevo of the Laboratory Facilities for Advanced Aquaculture Technology, SEAFDEC/AQD for proximate analysis, and Irene Cabanilla of SEAFDEC/AQD Finfish Hatchery. We also thank Dr. Yukio Hanamura of Japan International Research Center for Agricultural Sciences, Tsukuba, Japan for species identification of mysids.
Showing items related by title, author, creator and subject.
Book chapterMR Catacutan - In OM Millamena, RM Coloso & FP Pascual (Eds.), Nutrition in Tropical Aquaculture: Essentials of fish nutrition, feeds, and feeding of tropical aquatic species, 2002 - Aquaculture Department, Southeast Asian Fisheries Development CenterThe development of a feed that is both effective and economical for an aquaculture species in all its life stages is a continuous effort. Aquafeed development started when natural food sources in culture systems became inadequate and had to be supplemented with prepared feed. As fish stocking densities in culture increase, supplemental feeding is no longer sufficient. A complete feed that contains all the necessary nutrients in sufficient amounts to bring about good growth, survival, and reproduction is needed. Feed ingredients generally come from animal or plant sources and some are by-products of the food industry. There is no single feed ingredient or feedstuff that contains all the nutrients in adequate amounts. Thus, different feed ingredients are combined to make a feed that has the desired composition and nutrient levels. In combining various feed ingredients, it is important to know how much of each feed ingredient should be used to produce a cost-effective aquafeed. With the growth and expansion of aquaculture into a major industry, several fish species are being cultured; thus, the development of more efficient aquafeed formulations should continue. In developing cost-effective formulated diets, many important factors have to be considered. This chapter discusses these factors and the mathematical calculations in formulating a feed. It aims to enable students to formulate diets using purified and practical feed ingredients, and also to formulate effective supplemental and complete diets for aquaculture species.
Apparent digestibility of selected ingredients in diets for juvenile grouper, Epinephelus coioides (Hamilton) Apparent digestibility coefficients (ADCs) for dry matter (ADCdm) and crude protein (ADCcp) of selected feed ingredients were determined in vivo for grouper using passive faeces collection (Guelph System). A reference diet (RF) and test diets (consisted of 70% RF and 30% test ingredient) with 1% Cr2O3 as an inert indicator were used. An RF contained 45% protein, 10% fat and 15.7 kJ g−1 metabolizable energy. Three isonitrogenous and isocaloric diets, each contained a test ingredient (white fish meal, white cowpea meal and ipil-ipil leaf meal), were used in a growth study based on ADCcp of feed ingredients. An RF without Cr2O3 was a control. The ADC values of experimental diets were also determined. In grouper, the ADCdm of white cowpea meal, defatted soybean meal, wheat flour and shrimp meal (74–76%) were significantly lower than that of squid meal (99%), but comparable with those of the fish meals (84–89%). No significant difference was observed between the ADCdm of ipil-ipil leaf meal, rice bran and wheat flour (56–73%). The ADCcp of white cowpea meal and defatted soybean meal were similar to those of the fish meals, squid meal and shrimp meal (94–99%). The ADCcp of wheat flour was comparable with that of ipil-ipil leaf meal (79–83%). Rice bran had the lowest ADCcp value of 43%. Based on specific growth rate (SGR), the growth of fish fed white cowpea meal-based diets was similar to that of the control fish (3.2–3.3% day−1). Also, no significant difference was observed between the ADCdm (68–72%) and ADCcp (88–91%) of white cowpea meal-based diet and the control diet. The results suggest that ADC values can be used as indicators to determine the nutritional value of feed ingredients. White cowpea meal can be incorporated as a protein source in practical diet for grouper at 20.5% of the diet with no adverse effect on growth.
Apparent digestibility of selected feed ingredients in diets for grouper (Epinephelus coioides) juveniles PS Eusebio, RM Coloso & REP Mamauag - In MA Rimmer, S McBride & KC Williams (Eds.), Advances in grouper aquaculture, 2004 - Australian Centre for International Agricultural Research
Series: ACIAR Monograph 110This study was conducted to determine the quality of selected feed ingredients as protein sources in grouper diets, based on their nutrient composition and apparent digestibility coefficients for dry matter (ADMD) and crude protein (APD). A total of 56 juveniles were used for the 1st batch of test ingredients (Chilean fish meal, white fish meal, shrimp meal, defatted soyabean oilmeal, white cowpea meal and ipil-ipil leaf meal). 54, 72 and 48 juveniles were used for the 2nd, 3rd and 4th batches of test ingredients (squid meal, local meat and bone meal, meat solubles, soya protein concentrates and rice bran; tuna fish meal, imported meat and bone meal, blood meal, maize gluten meal and wheat flour; and poultry feather meal, lupin seed meal and maize germ meal, respectively). Apparent digestibility coefficients were measured in vivo. The apparent digestibility coefficients for ADMD ranged from 37-99%. Squid meal and meat solubles had the highest coefficients, whereas blood meal had the lowest. The APD of all feed ingredients tested were relatively high (79-99%), except for rice bran (43%) and blood meal (15%). ADMD values varied with the levels of fibre and other carbohydrate substances in the feed ingredients. Groupers could utilize dietary protein efficiently regardless of whether it was of animal or plant origin. High APD values were generally obtained in feed ingredients with high protein content. Low digestibility coefficients for feed ingredients could also be attributed to the processing methods used in their preparation.