Showing items related by title, author, creator and subject.

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  Evaluation of dietary freeze-dried Chaetoceros calcitrans supplementation to control Vibrio harveyi infection on Penaeus monodon juvenile 

  Seraspe, Ebonia B.; Gabotero, Shirleny; de la Peña, Milagros R.; Pahila, Ida G.; Amar, Edgar (Elsevier, 2014)

  Effects of supplementation of diets with freeze-dried Chaetoceros calcitrans to control Vibrio harveyi infection are evaluated through immune responses, and disease resistance of juvenile Penaeus monodon. Total lipid and fatty acid profile of Chaetoceros calcitrans is also analyzed. A challenge infection with 10⁷ cfu/mL concentration of Vibrio harveyi is intramuscularly injected to juvenile Penaeus monodon after 45 days of feeding of diets supplemented with 15 g/kg and 30 g/kg dried Chaetoceros calcitrans. The use of dried Chaetoceros calcitrans is compared with that of ß-1,3 glucan Curdlan, a commercial immune enhancer. Incorporation of 30 g/kg Chaetoceros calcitrans in the diet enhances the immune system of shrimp as effected by high prophenoloxidase activity and plasma protein concentration and is better compared to the commercially available Curdlan. Chaetoceros calcitrans also contains polyunsaturated fatty acids (PUFAs) such as linolenic acid and eicosapentanoic acid (EPA) which are responsible for its antibacterial action against Vibrio harveyi. All these biological activities of Chaetoceros calcitrans add up to increase resistance of the juvenile Penaeus monodon to vibriosis as shown by its high survival rate from the challenge infection with Vibrio harveyi. Therefore, it is worthwhile to use Chaetoceros calcitrans as supplementary feed. Its effect in increasing the immune competence coupled with its antibacterial action, make the shrimp resistant to luminous vibriosis that continues to affect the industry, thereby augmenting aquaculture production.
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  Advanced broodstock diets for the mangrove red snapper and a potential importance of arachidonic acid in eggs and fry 

  Emata, Arnil C.; Ogata, Hiroshi Y.; Garibay, Esteban S.; Furuita, Hirofumi (Springer Verlag, 2003)

  Mangrove red snapper fed advanced broodstock diets containing squid meal and squid oil exhibited higher hatching rates, cumulative survival and survival activity index than those fed a basal diet or a basal diet supplemented with mixture of antioxidants. On the other hand, fatty acid analyses of ovaries and fry of wild fish and eggs and larvae of broodstock fed raw fish revealed high arachidonic acid (ARA) and docosahexaenoic acid (DHA) levels and relatively lower eicosapentaenoic acid (EPA) levels consequently showing high ARA/EPA and DHA/EPA ratios compared to cold water species. This suggests that ARA may be nutritionally more important for egg and larval development and survival in tropical marine fish and its supplementation in broodstock diets may enhance reproductive performance of mangrove red snapper.
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  The essential fatty acid requirement of milkfish (Chanos chanos Forsskal) 

  Borlongan, Ilda G. (Springer Verlag, 1992)

  The essential fatty acid (EFA) requirement of milkfish was examined by a 12-week feeding trial using defined, purified diets at water temperature of 28–29°C and salinity of 32‰. The test diets contained varying levels of 18:0 (triglyceride form, TG), 18:3(n−3), 18:2(n−6) and (n−3) highly unsaturated fatty acids (n−3 HUFA). Milkfish juveniles were starved for 7 days and were than fed lipid-free diet for 30 days before the initiation of feeding trials. Low growth and feed efficiency together with high mortalities were observed in fish fed the lipid-free diet as well as in the EFA-deficient diet. Supplementation of 2% 18:2(n−6) to the tristearin based diet did not improve growth rate of milkfish as effectively as feeding with (n−3) fatty acids. The highest weight gain was obtained in milkfish fed a combination of 5% 18:0 + 1.0% 18:3(n−3) + 0.5% 20:5(n−3) + 0.5% 22:6(n−3) although the supplementation of 2% 18:3(n−3) alone or combination of 0.5% 20:5(n−3) + 0.5% 22:6(n−3) to the tristearin based diets were also effective for improvement of growth. Thus, (n−3) fatty acids, such as 18:3(n−3) and (n−3)HUFA were nutritionally more important than 18:2(n−6) for milkfish. The fatty acid composition of the polar lipids from whole body of milkfish juveniles fed the various test diets were influenced by the composition of the dietary fatty acids.