Molecular cloning and localization of GABAA receptor-associated protein in the rotifer Brachionus plicatilis
MetadataShow full item record
Cited times in Scopus
γ-Aminobutyric acid receptor type A-associated protein (GABARAP) and its homologs constitute a protein family found in many eukaryotes from yeast to human, and are known to be involved in intracellular membrane trafficking of GABAA receptors and autophagy. In this study, we cloned cDNA-encoding GABARAP from the monogonont rotifer Brachionus plicatilis and examined for its tissue distribution at the protein level in neonates, males and females. Using reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE) techniques, we showed that like other GABARAPs, rotifer GABARAP was also composed of 117 amino acids and highly homologous to vertebrate GABARAP2 ortholog (74–76% identity). GABARAP was demonstrated with its specific antibody to be ubiquitously distributed, irrespective of neonates, males, and females, in the coronal area that covers brain and contains most mechano- and chemoreceptors. Rotifer GABARAP was also expressed in the mature eggs but not in immature eggs. Double immunostaining with mammalian anti-GABA γ receptor antibody showed that rotifer GABARAP co-localized with GABA receptor, suggesting the association of the two proteins. The presence of GABARAP in rotifer implies that it is highly conserved during evolution, and plays important roles in various biological processes.
CitationMarcial, H. S., Suga, K., Kinoshita, S., Kaneko, G., Hagiwara, A., & Watabe, S. (2014). Molecular cloning and localization of GABAA receptor-associated protein in the rotifer Brachionus plicatilis.
Chemoreceptors; Antibodies; Marine invertebrates; Brain; Receptors; Proteins; Transcription; Hydrobiology; Evolution; Amino acids; Eggs; Reverse transcription; gamma -Aminobutyric acid receptors; GABARAP protein; Reviews; gamma -Aminobutyric acid A receptors; Membrane trafficking; Neonates; Phagocytosis; Yeasts; Amino acids; Membranes; Acids; Rotifera; Brachionus plicatilis; Rotifera
This study was partly supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS).
- Journal Articles 
Showing items related by title, author, creator and subject.
Evaluation of dietary freeze-dried Chaetoceros calcitrans supplementation to control Vibrio harveyi infection on Penaeus monodon juvenile Effects of supplementation of diets with freeze-dried Chaetoceros calcitrans to control Vibrio harveyi infection are evaluated through immune responses, and disease resistance of juvenile Penaeus monodon. Total lipid and fatty acid profile of Chaetoceros calcitrans is also analyzed. A challenge infection with 107 cfu/mL concentration of Vibrio harveyi is intramuscularly injected to juvenile Penaeus monodon after 45 days of feeding of diets supplemented with 15 g/kg and 30 g/kg dried Chaetoceros calcitrans. The use of dried Chaetoceros calcitrans is compared with that of ß-1,3 glucan Curdlan, a commercial immune enhancer. Incorporation of 30 g/kg Chaetoceros calcitrans in the diet enhances the immune system of shrimp as effected by high prophenoloxidase activity and plasma protein concentration and is better compared to the commercially available Curdlan. Chaetoceros calcitrans also contains polyunsaturated fatty acids (PUFAs) such as linolenic acid and eicosapentanoic acid (EPA) which are responsible for its antibacterial action against Vibrio harveyi. All these biological activities of Chaetoceros calcitrans add up to increase resistance of the juvenile Penaeus monodon to vibriosis as shown by its high survival rate from the challenge infection with Vibrio harveyi. Therefore, it is worthwhile to use Chaetoceros calcitrans as supplementary feed. Its effect in increasing the immune competence coupled with its antibacterial action, make the shrimp resistant to luminous vibriosis that continues to affect the industry, thereby augmenting aquaculture production.
Book chapterOM Millamena - In OM Millamena, RM Coloso & FP Pascual (Eds.), Nutrition in Tropical Aquaculture: Essentials of fish nutrition, feeds, and feeding of tropical aquatic species, 2002 - Aquaculture Department, Southeast Asian Fisheries Development CenterThis section aims to teach the reader the ten essential amino acids required by fish and their chemical structures, distinguish between essential and non-essential amino acids; the fate of absorbed amino acids in fish; effects of deficiencies and excesses of dietary amino acids in fish diets; the procedure on how to determine the qualitative and quantitative amino acid requirements of fish; methods of evaluating protein quality; and how to determine protein requirements of some aquaculture species.
Development of formulated feeds for grow-out culture of grouper (Epinephelus coioides) - tank and field studies OM Millamena & JD Toledo - In MA Rimmer, S McBride & KC Williams (Eds.), Advances in grouper aquaculture, 2004 - Australian Centre for International Agricultural Research
Series: ACIAR Monograph 110The objectives of this study were to compare the performance of a Southeast Asian Fisheries Development Centre (SEAFDEC) formulated diet with a commercial feed for growout culture of grouper and to transfer technology on grouper diet developed at SEAFDEC to the industry. In the tank study, Epinephelus coioides juveniles were reared in 12 units of 150-litre tanks at 15 fish/tank with 4 replicates per treatment. Fish were fed the diets at a feeding rate of 5-6% of body weight (BW) and trash fish at 10-12% BW per day for 60 days. In the feeding trial, treatments were arranged in a randomized complete block design with size groups as block. 36 fish were stocked per size group. Formulated feeds were given twice a day for 120 days. In the tank study, the commercial feed resulted to significantly lower growth, survival and food conversion ratio (FCR) compared with the SEAFDEC diet and trash fish control. Results of the field trials at growout ponds did not show significant differences in growth performance, survival and FCR of grouper juveniles fed with the diets. Both the SEAFDEC diet and commercial feed conformed to the established protein requirement of juvenile grouper. In tank trials, the poor performance of commercial feed was attributed to the low protein content and deficiencies in essential amino acids as confirmed by analysis of the amino acid composition. Improvement in growth performance of fish given the commercial feed was achieved in field trials by increasing the dietary protein level and improving the amino acid composition to match that of the grouper juveniles.