Molecular cloning and localization of GABAA receptor-associated protein in the rotifer Brachionus plicatilis
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2014著者
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2,020主題
chemoreceptors
antibodies
marine invertebrates
brain
receptors
proteins
transcription
hydrobiology
evolution
amino acids
eggs
reverse transcription
gamma -Aminobutyric acid receptors
GABARAP protein
literature reviews
gamma-aminobutyric acid
Membrane trafficking
phagocytosis
yeasts
membranes
acids
Brachionus plicatilis
Rotifera
young animals

antibodies

marine invertebrates

brain

receptors

proteins

transcription

hydrobiology

evolution

amino acids

eggs

reverse transcription

gamma -Aminobutyric acid receptors
GABARAP protein
literature reviews

gamma-aminobutyric acid

Membrane trafficking
phagocytosis

yeasts

membranes

acids

Brachionus plicatilis

Rotifera

young animals

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アイテムの詳細レコードを表示するCited times in Scopus
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γ-Aminobutyric acid receptor type A-associated protein (GABARAP) and its homologs constitute a protein family found in many eukaryotes from yeast to human, and are known to be involved in intracellular membrane trafficking of GABAA receptors and autophagy. In this study, we cloned cDNA-encoding GABARAP from the monogonont rotifer Brachionus plicatilis and examined for its tissue distribution at the protein level in neonates, males and females. Using reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE) techniques, we showed that like other GABARAPs, rotifer GABARAP was also composed of 117 amino acids and highly homologous to vertebrate GABARAP2 ortholog (74–76% identity). GABARAP was demonstrated with its specific antibody to be ubiquitously distributed, irrespective of neonates, males, and females, in the coronal area that covers brain and contains most mechano- and chemoreceptors. Rotifer GABARAP was also expressed in the mature eggs but not in immature eggs. Double immunostaining with mammalian anti-GABA γ receptor antibody showed that rotifer GABARAP co-localized with GABA receptor, suggesting the association of the two proteins. The presence of GABARAP in rotifer implies that it is highly conserved during evolution, and plays important roles in various biological processes.
Suggested Citation
Marcial, H. S., Suga, K., Kinoshita, S., Kaneko, G., Hagiwara, A., & Watabe, S. (2014). Molecular cloning and localization of GABAA receptor-associated protein in the rotifer Brachionus plicatilis. International Review of Hydrobiology , 99(1-2), 188-197. https://doi.org/10.1002/iroh.201301720
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ArticleISSN
1434-2944; 1522-2632Collections
- Journal Articles [1163]
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