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Molecular cloning and localization of GABAA receptor-associated protein in the rotifer Brachionus plicatilis

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日付
2014
著者
Marcial, Helen S.
Suga, Koushirou
Kinoshita, Shigaheru
Kaneko, Gen
Hagiwara, Atsushi
Watabe, Shugo
Page views
2,020
主題
chemoreceptors ASFA
antibodies ASFA
marine invertebrates ASFA
brain ASFA
receptors ASFA
proteins ASFA
transcription ASFA
hydrobiology ASFA
evolution ASFA
amino acids ASFA
eggs ASFA
reverse transcription ASFA
gamma -Aminobutyric acid receptors
GABARAP protein
literature reviews ASFA
gamma-aminobutyric acid ASFA
Membrane trafficking
phagocytosis ASFA
yeasts ASFA
membranes ASFA
acids ASFA
Brachionus plicatilis AGROVOC
Rotifera AGROVOC
young animals AGROVOC
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抄録
γ-Aminobutyric acid receptor type A-associated protein (GABARAP) and its homologs constitute a protein family found in many eukaryotes from yeast to human, and are known to be involved in intracellular membrane trafficking of GABAA receptors and autophagy. In this study, we cloned cDNA-encoding GABARAP from the monogonont rotifer Brachionus plicatilis and examined for its tissue distribution at the protein level in neonates, males and females. Using reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE) techniques, we showed that like other GABARAPs, rotifer GABARAP was also composed of 117 amino acids and highly homologous to vertebrate GABARAP2 ortholog (74–76% identity). GABARAP was demonstrated with its specific antibody to be ubiquitously distributed, irrespective of neonates, males, and females, in the coronal area that covers brain and contains most mechano- and chemoreceptors. Rotifer GABARAP was also expressed in the mature eggs but not in immature eggs. Double immunostaining with mammalian anti-GABA γ receptor antibody showed that rotifer GABARAP co-localized with GABA receptor, suggesting the association of the two proteins. The presence of GABARAP in rotifer implies that it is highly conserved during evolution, and plays important roles in various biological processes.
URI
http://hdl.handle.net/10862/2250
Suggested Citation
Marcial, H. S., Suga, K., Kinoshita, S., Kaneko, G., Hagiwara, A., & Watabe, S. (2014). Molecular cloning and localization of GABAA receptor-associated protein in the rotifer Brachionus plicatilis. International Review of Hydrobiology, 99(1-2), 188-197. https://doi.org/10.1002/iroh.201301720 
DOI
10.1002/iroh.201301720
Type
Article
ISSN
1434-2944; 1522-2632
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  • Journal Articles [1163]

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