GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis
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γ-Aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) have been shown to increase the reproduction of the Brachionus plicatilis (NH3L strain). In the present study, the endogenous presence of GABA and 5-HT in the rotifers B. plicatilis (NH3L and Kamiura strains) and Brachionus rotundiformis (Langkawi strain) were confirmed by dot blot immunoassay and high-performance liquid chromatography (HPLC). HPLC showed that GABA and 5-HT concentrations in the three rotifer strains range from 71 to 188 pmol/mg and from 12 to 64 pmol/mg, respectively. A total of 33 amino acids were also detected in B. plicatilis and B. rotundiformis, with glutamic acid, serine, glycine, taurine, threonine, alanine, arginine, proline, valine and isoleucine in high concentrations relative to other amino acids.
CitationGallardo, W. G., Hagiwara, A., Hara, K., Soyano, K., & Snell, T. W. (2000). GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis.
This study was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (No. 10660187) and Integrated Research Program for Effects of Endocrine Disrupters on Agriculture, Forestry, and Fisheries and their Action Mechanisms on Domestic Animals and Fishes (ED-99-II-3-1).
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Conference paperAC Fermin - In JV Juario & LV Benitez (Eds.), Seminar on Aquaculture Development in Southeast Asia, 8-12 September 1987, Iloilo City, Philippines, 1988 - Aquaculture Department, Southeast Asian Fisheries Development CenterBighead (Aristichthys nobilis) and silver (Hypophthalmichthys molitrix) carps were reared in ponds, pens and floating cages in Laguna Lake until maturity. Spontaneous gonadal maturation and rematuration of carp broodstock occurred within 2-2.5 years with average weight of 3-4 kg. Under lake conditions, broodstock were not given supplemental feeds. Induced spawning of gravid females was done by intraperitoneal injections using HCG combined with either common carp pituitary homogenates or LHRH-A. Stripping and dry-fertilization of eggs were done 6-8 hr after the final injection. Eggs were incubated in water containing 300-500 ppm Total Hardness. Fertilization and hatching rates were 23-88% and 7-36%, respectively. Post-larval carps were reared in tanks and fine-meshed nylon net cages installed in manured ponds. Tank-reared post-larvae were fed with Brachionus plicatilis and subsequently with Moina macrocopa in combination with powered formulated feeds containing 40% crude protein. Fry were harvested and stocked in nursery cages after 30-45 days of rearing in tanks. Four-month old 50-100 g tilapia (Oreochromis niloticus) stocked in hapa net cages, tanks or ponds were used for breeding. Egg and fry production was significantly high at 4 females/m2 stocking density Different sex ratios, however, did not affect fry production. Spawning frequency and total growth of broodstock was highest in fry fed formulated diets containing 50% crude protein. Harvesting of fry was done every 15 days during summer months and every 21 days during cold months. Fry were reared in tanks and hapa cages and fed diets containing 35% crude protein. Supplemental feeding in the lake was suspended when productivity reached 3 gC/m2 /day.
Feeding selectivity of the seahorse, Hippocampus kuda (Bleeker), juveniles under laboratory conditions FT Celino, GV Hilomen-Garcia & AGC del Norte-Campos -
Aquaculture Research, 2012 - Blackwell Publishing LtdThis study examined the feeding selectivity of Hippocampus kuda juveniles under captive conditions and evaluates different food organisms that could be used to improve hatchery-rearing of this species. Newly born H. kuda were reared for 10 days in 60-L capacity tanks and fed rotifers (Brachionus rotundiformis), zooplankton (mostly Pseudodiaptomus annandalei and Acartia tsuensis) alone or both food sources. The size and amount of food ingested increased as seahorses grew. Selective feeding of seahorses appeared to change as they develop, preferring copepod adults over nauplii and rotifers. A. tsuensis was highly selected by juveniles over P. annandalei. Specific growth rate in terms of body weight (SGR-BW, 15% day–1) was the highest and mortality rate (9% at day 10) the lowest in seahorses fed a mixed food sources. Slowest growth rate (0.3% day–1) and highest mortality rate (60% at day 7) were observed in seahorses fed rotifers alone. These results indicate that copepods are suitable food for seahorse juveniles, but a mixture of food organisms in the rearing tank environment enhances survivorship and growth of H. kuda, thus potentially providing a source of cultured rather than wild specimens for characterizing the life history of this threatened species.
Morphological development and survival of Philippine silver therapon larvae, Leiopotherapon plumbeus (Kner, 1864) reared under different feeding schemes JAP Añano & MRR Eguia - In Proceedings of the DLSU Research Congress 2016, De La Salle University, Manila, Philippines, March 7-9, 2016, 2016 - De La Salle UniversityPhilippine silver therapon, Leiopotherapon plumbeus, locally known as ayungin, is an endemic fish species in the country. The demand for silver therapon remained high despite the decline of its population and commercial catches. Taking into account the potential of this species for stock enhancement, this study focused on developing an initial feeding strategy necessary to provide potential L. plumbeus juveniles for aquaculture. The experiment conducted was a short duration 10-day feeding experiment which used different feeding schemes utilizing live food organisms [Brachionus (B), Moina (M), co-fed with BM, Chlorella (C) and filtered lake water (L)]. Morphometric measurements were evaluated to assess growth performance of larvae among different feeding schemes. Survival rate was determined at the end of feeding experiment at 9 DAH (days after hatching). Growth parameters of L. plumbeus larvae reared under different feeding schemes showed significant differences starting at 5 DAH (P < 0.05) onward. After a total of 10 days, a significant decline in fish larval samples was observed under two feeding schemes (C and L). BM has a significant higher rate of survival (18.07 ± 7.09%) compared to other treatments. B. rotundiformis (15-20 mL-1) can be considered as a starter food for silver therapon larviculture and M. micrura (5-10 mL-1) can be utilized during an assumed co-feeding phase at 6 DAH.