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dc.contributor.authorMarcial, Helen S.
dc.contributor.authorSuga, Koushirou
dc.contributor.authorKinoshita, Shigaheru
dc.contributor.authorKaneko, Gen
dc.contributor.authorHagiwara, Atsushi
dc.contributor.authorWatabe, Shugo
dc.date.accessioned2015-05-25T02:08:07Z
dc.date.available2015-05-25T02:08:07Z
dc.date.issued2014
dc.identifier.citationMarcial, H. S., Suga, K., Kinoshita, S., Kaneko, G., Hagiwara, A., & Watabe, S. (2014). Molecular cloning and localization of GABAA receptor-associated protein in the rotifer Brachionus plicatilis. International Review of Hydrobiology, 99(1-2), 188-197.en
dc.identifier.issn1434-2944
dc.identifier.urihttp://hdl.handle.net/10862/2250
dc.description.abstractγ-Aminobutyric acid receptor type A-associated protein (GABARAP) and its homologs constitute a protein family found in many eukaryotes from yeast to human, and are known to be involved in intracellular membrane trafficking of GABAA receptors and autophagy. In this study, we cloned cDNA-encoding GABARAP from the monogonont rotifer Brachionus plicatilis and examined for its tissue distribution at the protein level in neonates, males and females. Using reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE) techniques, we showed that like other GABARAPs, rotifer GABARAP was also composed of 117 amino acids and highly homologous to vertebrate GABARAP2 ortholog (74–76% identity). GABARAP was demonstrated with its specific antibody to be ubiquitously distributed, irrespective of neonates, males, and females, in the coronal area that covers brain and contains most mechano- and chemoreceptors. Rotifer GABARAP was also expressed in the mature eggs but not in immature eggs. Double immunostaining with mammalian anti-GABA γ receptor antibody showed that rotifer GABARAP co-localized with GABA receptor, suggesting the association of the two proteins. The presence of GABARAP in rotifer implies that it is highly conserved during evolution, and plays important roles in various biological processes.en
dc.description.sponsorshipThis study was partly supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS).en
dc.language.isoenen
dc.publisherWiley-VCH Verlagen
dc.subjectBrachionus plicatilisen
dc.subjectRotiferaen
dc.subjectyoung animalsen
dc.subjectgamma-aminobutyric aciden
dc.titleMolecular cloning and localization of GABAA receptor-associated protein in the rotifer Brachionus plicatilisen
dc.typeArticleen
dc.identifier.doi10.1002/iroh.201301720
dc.citation.volume99
dc.citation.issue1-2
dc.citation.spage188
dc.citation.epage197
dc.citation.journalTitleInternational Review of Hydrobiologyen
dc.subject.asfachemoreceptorsen
dc.subject.asfaantibodiesen
dc.subject.asfamarine invertebratesen
dc.subject.asfabrainen
dc.subject.asfareceptorsen
dc.subject.asfaproteinsen
dc.subject.asfatranscriptionen
dc.subject.asfahydrobiologyen
dc.subject.asfaevolutionen
dc.subject.asfaamino acidsen
dc.subject.asfaeggsen
dc.subject.asfareverse transcriptionen
dc.subject.asfaliterature reviewsen
dc.subject.asfagamma-aminobutyric aciden
dc.subject.asfaphagocytosisen
dc.subject.asfayeastsen
dc.subject.asfamembranesen
dc.subject.asfaacidsen
dc.identifier.essn1522-2632
local.subjectγ-Aminobutyric aciden
local.subjectGABARAP orthologsen
local.subjectNeurotransmitteren
local.subjectRotiferaen


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    These papers were contributed by Department staff to various national and international journals.

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