Quality assessment of newly hatched mud crab, Scylla serrata, larvae
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Starvation and exposure to formalin were investigated as possible stress tests for evaluating the quality of mud crab, Scylla serrata, larvae. For the starvation stress test, newly hatched zoeae stocked in 150-ml containers were either starved or fed rotifers. Similarly, newly hatched zoeae were stocked in containers with seawater of 0 (control), 20, 30 and 40 mg/L formalin for the formalin stress test. The zoeae from the same batches were used for seed production to monitor their performance and validate the results of stress tests. Starvation was found to be unsuitable for larval quality evaluation. However, the impact of initial food deprivation on the newly hatched larvae indicates that feeding immediately after hatching is necessary for mud crab larvae. Exposure of larvae to 40 mg/L formalin for 3 hr appeared to be a reliable and practical method for larval quality assessment as the survival of larvae in the mass production tanks validated the classification of good and poor quality batches in the stress tests. On this basis, a hatchery operator can decide which batch should be cultured further. Finally, there appears to be a link between the quality of larvae and the performance at the megalopa and early juvenile crabs.
CitationQuinitio, E. T., dela Cruz-Huervana, J. J., & Parado-Estepa, F. D. (2018). Quality assessment of newly hatched mud crab, Scylla serrata, larvae.
Aquatic crustaceans; Food availability; Marine environment; Containers; Starvation; Evaluation; Seed production; Juveniles; Larvae; Quality control; Tests; Hatching; Quality; Hatcheries; Sea water; Zoeae; Biological stress; Tanks; Scylla serrata; Formaldehyde; Formalin; Larval quality; Mud crab hatchery
This study (Br-01 C2007 and Br-03 C-2010) was supported by the SEAFDEC/AQD and Trust Fund of the Government of Japan and PCAARRD-Department of Science and Technology. The authors express their gratitude to the Crustacean Hatchery staff for the technical assistance.
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