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Draft genome sequence of Vibrio parahaemolyticus strain PH1339, which causes acute hepatopancreatic necrosis disease in shrimp in the Philippines
(American Society for Microbiology, 2019-11-14)
We report the first draft genome sequence of an acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus strain isolated from a Penaeus vannamei sample from the Philippines. The strain carries the ...
Draft genome sequence of multidrug-resistant Vibrio parahaemolyticus strain PH698, infecting penaeid shrimp in the Philippines
(American Society for Microbiology, 2019-11-21)
The emergence of multidrug-resistant bacterial strains in diverse settings has been reported globally. In the Philippine shrimp aquaculture industry, antibiotics are used for the treatment of bacterial diseases during the ...
Mortality of pond-cultured Litopenaeus vannamei associated with acute hepatopancreatic necrosis disease (AHPND) and white spot syndrome virus (WSSV) infection in the Philippines
(Japanese Society of Fish Pathology, 2017)
Mortalities of up to 60% were observed in pond-cultured Litopenaeus vannamei in Bohol, Philippines. Histopathological examination revealed typical acute hepatopancreatic necrosis disease (AHPND) pathology. PCR test generated ...
Antibacterial properties of the microalgae Chaetoceros calcitrans
(Asian Fisheries Society, 2012)
The antibacterial properties of the microalgae Chaetoceros calcitrans were assessed. Samples of C. calcitrans were first extracted in methanol, and then in different organic solvents of increasing polarity, n-hexane (n-Hex), ...
Induction of immunity and resistance to white spot syndrome virus (WSSV) in shrimp Penaeus monodon (Fabricius) by synthetic oligodeoxynucleotide and bacterial DNA
(University of the Philippines Los Baños, 2012)
Shrimps like all invertebrates are believed to lack true adaptive immunity but recent evidence indicate that they can be protected against pathogenic organisms by priming their immune system with immunostimulatory substances. ...
Surveillance of emerging fish viral pathogens in some Southeast Asian countries
(Society of Israeli Aquaculture and Marine Biotechnology, 2009)
Preventing the transboundary movement of fish viral pathogens in a global environment requires active surveillance. This study examined the presence of three emerging viral pathogens among koi, common, grass, and silver ...
Acute hepatopancreatic necrosis disease (AHPND) outbreaks in Penaeus vannamei and P. monodon cultured in the Philippines
(Inter Research, 2015)
Acute hepatopancreatic necrosis disease (AHPND) has recently emerged as a serious disease of cultured shrimp. It has also been described as early mortality syndrome (EMS) due to mass mortalities occurring within 20 to 30 ...
Genomic analysis of Vibrio harveyi strain PH1009, a potential multi-drug resistant pathogen due to acquisition of toxin genes
(Elsevier, 2023-03-25)
In has increasingly been observed that viral and bacterial coinfection frequently occurs among cultured shrimp and this coinfection could exacerbate the disease phenotype. Here, we describe a newly discovered bacterial strain, Vibrio harveyi PH1009 collected from Masbate Island, Philippines that was found to be co-infecting with the White Spot Syndrome virus a sample of black tiger prawn, Penaeus monodon. The genome of V. harveyi PH1009 was sequenced, assembled, and annotated. Average Nucleotide identity calculation with Vibrio harveyi strains confirmed its taxonomic identity. It is a potential multi-drug and multi-heavy metal resistant strain based on the multiple antibiotic and heavy metal resistance determinants annotated on its genome. Two prophage regions were identified in its genome. One contained genes for Zona occludens toxin (Zot) and Accessory cholera toxin (Ace), essential toxins of toxigenic V. cholerae strains apart from CTX toxins. Pan-genome analysis of V. harveyi strains, including PH1009, revealed an “open” pan-genome for V. harveyi and a core genome mainly composed of genes necessary for growth and metabolism. Phylogenetic tree based on the core genome alignment revealed that PH1009 was closest to strains QT520, CAIM 1754, and 823tez1. Published virulence factors present on the strain QT520 suggest similar pathogenicity with PH1009. However, PH1009 Zot was not found on related strains but was present in strains HENC-01 and CAIM 148. Most unique genes found in the PH1009 strain were identified as hypothetical proteins. Further annotation showed that several of these hypothetical proteins were phage transposases, integrases, and transcription regulators, implying the role of bacteriophages in the distinct genomic features of the PH1009 genome. The PH1009 genome will serve as a valuable genomic resource for comparative genomic studies and in understanding the disease mechanism of the Vibrio harveyi species....
Whole-genome sequencing for identification of bacterial isolates affecting local penaeid shrimp farms: A national survey for the Philippines
(5M Books Ltd, 2022)
The shrimp aquaculture industry is a major economic player in the food and livelihood sectors, both globally and in the Philippines. The development of industrialized shrimp farming methods has led to a rapid increase in ...
In vitro and in vivo evaluation of the efficacies of commercial probiotics and disinfectant against acute hepatopancreatic necrosis disease and luminescent vibriosis in Litopenaeus vannamei
(Elsevier, 2024-05)
The bioactivities of two commercially available probiotics and one chemical disinfectant were tested against strains of Vibrio parahaemolyticus (VP<sub>AHPND</sub>) and V. harveyi. This study aimed to determine shrimp pathogenic Vibrios' in vitro and in vivo sensitivities to commercial probiotics and a chemical disinfectant. The probiotics and disinfectant were tested first in vitro, followed by the in vivo trials. Results showed that upon administration of probiotics either through diet or adding into the tank water, the survivability of shrimp was increased during challenge with VP<sub>AHPND</sub> and V. harveyi. Also, the disinfectant was tested against the same pathogens and showed positive bactericidal effects at 2500 ppm and 5000 ppm. The present findings suggest that adding probiotics to the rearing water or the shrimp feeds effectively prevents infection by lowering the load of pathogenic bacteria. In comparison, the effectiveness of the disinfectant (PUR) depends on its appropriate concentration and timing of application. It is not only limited to rearing water but is also applicable for decontaminating pond liners, tanks, and other paraphernalia....