Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff: Recent submissions
1301-1320 / 1693
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Ecology on the feeding of milkfish fry and juveniles, Chanos chanos (Forsskal) in the Philippines
(Kagoshima University Research Center for the South Pacific, 1980)An extensive ecological survey was conducted in the Philippines to study the feeding of milkfish, Chanos chanos Forsskal. Types of environments examined included coral reefs, lagoons, mangrove and nipa swamps as well as estuarine systems. It was concluded that organic detritus was the basic nutrient for juvenile milkfish and that depositional environments constitute important nursery and feeding grounds for this spcies. The main pathway of energy flow in these coastal ecosystems through the detritus rather than the grazing pathway. The feeding of milkfish was described in terms of habitat structures, stomach content, feeding chronology and feeding behaviour. The result suggests that a better understanding of the function of the ecosystem will help to improve present aquaculture practices as well as guidelines for resource management. -
Early postmysis stages of the giant tiger prawn, Penaeus monodon Fabricius
(Carcinological Society of Japan, 1980)The morphological development of the early postmysis stages of the giant tiger prawn, Penaeus monodon are described and illustrated using laboratory-reared specimens and wild ones. The first 3 or 4 substages are termed "megalopa" owing to the incomplete branchial formula of the mouth parts, their pelagic or planktonic behavior and their somewhat narrower body. At the 4th or 5th substage, the branchial formula is completed, their behavior is more or less epibenthic, the chromatophores are densely distributed from the tip of the antennular flagella through the ventral side of the abdomen to the tip of the telson. At the beginning of the 7th substage, the chromatophores are distributed over almost the whole body. Thus from the 4th or 5th substage onwards they may be termed juveniles. The main characteristics development are as follows : 1) The cempletion of the rostral spine formula appears in the 7th or 8th substage showing 7 dorsal and 3 ventral spines. 2) The endopod of the 1st maxilliped develops again at the 7th substage, although it degenerates with every molt prior to the 7th. 3) From the 1st substage, the distal 3 segments of the endopod of the 2nd maxilliped are bent sharply inwards. -
Culture experiments on the harpacticoid copepod, Tisbintra elongata Mori, and evaluation of that species as a food organism for milkfish larvae
(Faculty of Fisheries, Kagoshima University, 1980)The mass culture of the harpacticoid copepod, Tisbintra elongata, indigenous to Panay Island were conducted, feeding them on several kinds of food materials. Salinity tolerance as physiological parameter was also examined. At the same time their efficiency as food for milkfish larvae was evaluated. The highest density of 10.5 individuals/m/ was obtained in copepods fed on rice bran and fermented fish solubles at rates of 0.125 to 0.25 and 0.16 mg/indiv./day respectively. The provision of shelter as habitat was also supplementary for growth. This species of copepod was found to be euryhaline, and could grow to high densities in waters hypersaline to their natural habitat. Statistically, no significant difference of growth was observed between the milkfish larvae fed on this species and Artemia nauplius. However, comparatively stable results were obtained using Tisbintra as food. In this rearing of milkfish larvae, those larvae whose sizewas 12.3 to 13.5mm in body length were considered to be just prior to morphological change. -
A fundamental study on the behavior of milkfish fry for improving the efficiency of traditional fry collecting gear in the Philippines
(Kagoshima University Research Center for the South Pacific, 1980)The reaction of milkfish fry to miving and stationary nets of different meshes and colors in an experimental tank was determined. The underwater visibility of the nets saw measured and the water filtration in a fry-sweeper was observed. Milkfish fry were both driven well by the moving nets and retained well by the stationary nets, with the fine-meshed black net most effective in both cases. The white and blue nets were found to be quite invisible to the fry in the blue-painted tank, particularly under contour lighting conditions; the black net was found to be very visible to the fry under both surface and contour light. The underwater visibility of the nets was found to vary with the sea conditions and the light direction. Water filteration in the fry-sweeper was found to be almost perfect. From the results, it was concluded that milkfish fry are caught by the moving fry collecting gear through driving and not by filtering. Since fry collection grounds are usually turbid, it was recommended that dark-colored materials be used for effective driving. It is also deemed much better to use larger mesh nets in the wings of the fry gear to minimize net resistance in the water and facilitate operation. -
Relationship between diet composition and growth rate of the zoeal and mysis stages of Penaeus japonicus Bate
(Fisheries Research Society of the Philippines, 1979)Feeding experiments were conducted on Penaeus japonicus larvae using Chaetoceros gracilis plus Artemia nauplii, an artificially prepared diet (Diet-B), and two commercial feeds (Tapes and mysid meals) in a randomized complete block design with two replicates per diet. The artificially prepared diets were dried and ground to size 10 to 50 microns and fed to the larvae. The larvae were reared in round plastic aquaria each containing 5 liters of filtered seawater. Each aquarium was stocked with 250 larvae and provided with aeration. The larvae were reared from zoea (Z1) to mysis (M3) stage and growth was measured daily. The highest survival rate of 34.2% was obtained when the larvae were fed with Diet-B. C. gracilis plus Artemia nauplii feeding gave a survival rate of 21.6%. On the other hand, growth measured in terms of development, was fastest using C. gracilis plus Artemia nauplii. Larvae metamorphosed into stage in 7 days with an average gain in length of 0.46 mm/day. Diet-B feeding resulted in a comparable growth, the larvae reaching M3 stage in 8 days with an average gain in length of 0.30 mm/day. Analyses of the chemical composition of the diets showed no definite relationship between diet composition and growth and survival rates of the early larval stages of P. japonicus. Results obtained in this study demonstrate that the early larval stages of P. japonicus can be reared with feeding of Diet-B. Since the chemical composition of the diet is known, it can be used as supplemental data for larval feed development and nutritional requirements studies for the early larval stages of P. japonicus and/ or other penaeids. -
Feeding regimes in relation to reproduction and survival of ablated Penaeus monodon
(University of the Philippines at Los Baños, 1979)Pond-reared P. monodon were stocked in four 12-cu m flowthrough maturation tanks at 25 males and 50 females per tank; females were unilaterally ablated. The combinations of feeds for the morning and afternoon rations were pellet-pellet, frozen mussel-frozen mussel, frozen mussel-pellet, and frozen squid-pellet. Reproductive performance in terms of total number of spawnings, total number of eggs produced, total number of nauplii produced, average number of eggs per spawning and average hatching rate was best for the mussel-pellet combination followed by the mussel-mussel and squid-pellet rations. The all pellet diet gave the poorest results. -
Growth and survival of Penaeus monodon postlarvae fed shrimp head meal and fish meal as primary animal sources of protein
(Fisheries Research Society of the Philippines, 1979)Penaeus monodon postlarvae with mean weights of 2 to 3 g were fed shrimp head meal (Diet B), fish meal (Diet C), mussel (Diet E), a combination of equal amounts of shrimp head meal and fish meal (Diet A) or one part shrimp head meal and 2 parts fish meal (Diet D) as primary sources of protein. The larvae were reared for 30 days in cylindrical fiberglass tanks containing 150 l of aerated seawater. Mean weight gains were significantly highest among those fed combinations ofish meal and shrimp head meal. Diets A and D, 0.88 g and 0.59 g, respectively. Mean weight gains among those fed one main source of protein, Diets B, C and E were not significantly different from each other and were very low. Highest survival rate was observed among hose fed Diet C (85%) and the lowest recorded (45%) was obtained from those fed mussel. Those fed Diet B grew the slowest and had the lowest survival rate of the groups given formulated diets. Shrimp head meal and fish meal complement each other in the diet formulations. Shrimp head meal alone does not provide for growth and survival, but fish meal can provide for high survival rate. The addition of shrimp head improves the diet. Cholesterol which is present in shrimp could have caused the difference. -
On the new species of Penaeus (Crustacea, Decapoda: Penaeidae) from North Borneo
(Carcinological Society of Japan, 1979)A new species of penaeid prawn, Penaeus silasi from North Borneo is described in detail and compared with the closely related species such as P. indicus, P. merguiensis and P. penicillatus. The new species is characterized by the following features : The 3rd maxilliped of the adult male has a dactylus as long as or slightly shorter than the propodus which has only a rudimentary tuft of hair instead of a long tuft of bristles as in P. indicus; the anterior plate of the thelycum is triangular and relatively prominent. -
On a new species of Trachypenaeus (Crustacea, Decapoda: Penaeidae) from the Philippines, with notes on related species
(Carcinological Society of Japan, 1979)Trachypenaeus villaluzi a new species of penaeid prawn from the Philippines is described in detail and compared with the related species. The new species is characterized by the following features : Mastigobranchia are present on the 2nd and 3rd pereiopods, but absent on the first ; anterior plate of thelycum is deeply concave ; the lower margin of the distolateral projections of the petasma is concave. It is also suggested that Trachypenaeus asper Alcock Should be considered as a valid species instead of being treated as a synonym of T. curvirostris (Stimpson). -
Effects of crude, semipurified, and purified starch of sago (Metroxylon sagu Rottb.) on the water stability of pelleted shrimp diets
(Fisheries Research Society of the Philippines, 1979)Three isonitrogenous, isocaloric practical-type diets with purified, semipurified and crude starch from sago palm as binders, were pelleted through a 2-mm diameter die in a Hobart meat grinder, steamed for 5 min at 85 to 90 C and oven-dried to a moisture content of approximately 10%. The pellets were evaluated for water stability after 3, 6 and 21 h in seawater with 32 ppt salinity and 28 C temperature. Approximately equal amounts of the 3 diets remained intact after 3 and 6 h. After 12 h of immersion the pelleted diets decreased significantly in water stability. However, the diet containing semipurified sago starch had the highest water stability (79.1%). The values were nearly the same for the pellets bound with purified and crude sago starch. Purified sago had the highest additional cost per kg of diet (P4.15). The semipurified and the crude sago starch had about the same costs, at P0.17 and P0.135, respectively. Semipurified sago starch, therefore, is preferable to the crude sago starch. -
Results of drift card experiments and considerations on the movement of milkfish eggs and larvae in the northern Sulu Sea
(Fisheries Research Society of the Philippines, 1979)For a period of one year beginning December 1977, drift card experiments were conducted off the western and southwestern coasts of Panay Island to determine the surface currents in the area. Of a total 2,384 drift cards released during the study, 382 (16.02%) were recovered, 92% of them within 30 days following dispatch. The surface currents in the study area are strongly influenced, in direction and speed, by the prevailing monsoon winds. During the NE monsoon period, the surface currents move away from the coast; during the SW monsoon, toward and/or parallel to the coast. Based on the results, the probable movement and transport of milkfish eggs and larvae from the spawning ground to the fry collection ground are also discussed. -
Notes on Lagenidium from larvae of Penaeus monodon Fabricius. I. Isolation and culture in artificial media
(Fisheries Research Society of the Philippines, 1979)The fungus, tentatively identified as Lagenidium , which attacks the nauplii to postlarval stages of P. monodon , has recently been isolated and cultured from infected larvae under laboratory conditions, using several kinds of agars and broths. Growth of the colonies was found to be faster in the broths and in the Sabouraud agar ghan in the othr media. Observations were made on the variations in sizes of the vegetative and reproductive structures and on the manner of sporulation to form the basis of identification. -
Studies on the fungal diseases in crustaceans. I. Lagenidium scyllae sp. nov. isolated from cultivated ova and larvae of the mangrove crab (Scylla serrata)
(Mycological Society of Japan, 1979)Lagenidium scyllae , a marine mastigomycete from the ova and larvae of the mangrove crab, is described and illustrated as new. The fungus grew at a temperature range of 16-42 C, with an optimum at 22.5-31.8 C. It grew well in peptone-yeast-glucose (PYG) broth containing 2-3% NaCl. In PYG-sea water medium, it grew at a pH range of 5-11. -
Settlement preference of the brown mussel, Modiolus metcalfei, Hanley and its implication on the aquaculture potential of the species
(Fisheries Research Society of the Philippines, 1978)A study on the settlement preference of M. metcalfei was made in Banate Bay, Iloilo, using four types of materials as spat collectors. During the six-month study period, not a single Modiolus spat was found in any of the materials tested. Spats were found attached to the posterior half of living adult mussels collected for related studies. Tests with empty Modiolus shells and bamboo fish corrals as spat collectors showed negative results indicating that settlement response in the brown mussel is elicited by the presence of living animals. -
Preliminary studies on growth and survival of Penaeus japonicus postlarvae fed with Tapes philippinarum and commercial formula feed.
(Fisheries Research Society of the Philippines, 1978)The postlarvae were maintained in 5-l round plastic aquaria and provided with aeration for a 28-day feeding period. Highest survival rate of 100% was obtained when the postlarvae were fed with Diet-B. Growth, in terms of weight and length was highest in postlarvae fed with Kyowa Hakko and mysid feeds. Using T. philippinarum as control, this experiment showed that feeding with Diet-B, Kyowa Hakko and mysid feeds resulted in similar or better growth and survival rates of P. japonicus postlarvae. -
Mass production in concrete tanks of sugpo Penaeus monodon Fabricius spawners by eyestalk ablation
(Fisheries Research Society of the Philippines, 1978)Two batches of 196 and 181 P. monodon females were ablated on one eyestalk in March and July 1977, respectively, and a control batch of 180 unablated females was stocked in July 1977. The females were stocked with males in 120-ton concrete tanks with partial flowthrough of water and fed salted mussel (Modiolus metcalfei ) at 15% body weight daily. Total number of spawnings was 82 for the March-ablated group, 3 for the July-ablated group and 4 for the July unablated group. Maturation and mortality in relation to the moult cycle and tank plus water conditions are discussed. -
Present prawn culture in the Philippines.
(Bureau of Fisheries and Aquatic Resources, 1978)This paper discusses present prawn culture practices in the Philippines, as well as the experiments which are conducted at the SEAFDEC Aquaculture Department by reviewing production of fry, production of juveniles in nursery ponds, growing prawn in rearing ponds, and present problems of prawn culture in the Philippines. -
A study on certain aspects on the biology and control of Caligus sp., an ectoparasite of the adult milkfish Chanos chanos (Forskal)
(Fisheries Research Society of the Philippines, 1978)One unidentified species of copepod belonging to the genus Caligus was found to infest the adult milkfish broodstock kept in canvas tanks at the SEAFDEC research station in Mag-aba, Pandan, Antique, Philippines. This report describes the morphology of the adult and early larval stages of this copepod. A highly effective method of control using the chemical Neguvon (2,2,2-trichloro-1-hydroxyl-phosphoric acid-dimethylethol) at a concentration of 0.25 ppm is recommended. -
Use of hormones in breeding cultivated warm-water fishes with special reference to milkfish, Chanos chanos (Forskal)
(Fisheries Research Society of the Philippines, 1978)The role of hormones in the controlled reproduction of a few test fishes is well documented. However, information on the mechanisms of endocrine regulation of ovulation in cultivated warm-water fishes is very meagre. Hormones, especially the gonadotropic hormones of piscine origin, are increasingly being used in modern aquaculture to produce the seed of many important cultivated fishes. While chorionic gonadotropin and other exogenous mammalian hormones are used in spawning the channel catfish, fish pituitary hormones are usually needed to induce spawning in the difficult-to-spawn Asiatic carps. In mullets, however, either homoplastic pituitary gland or human chorionic gonadotropin (HCG) or a mixture of HCG and a threshold dose of the former is ordinarily injected to precipitate spawning. Of late, semi-purified salmon gonadotropin (SG-G100) has been used to induce spawning in several species of food fishes. While several marine fishes have been artificially bred by administration of hormones, induced spawning of the milkfish, C. chanos has been tried with little success. The milkfish is a widely distributed food fish extensively cultivated in ponds in Southeast Asia. Recently, significant results have been obtained in spawning mature milkfish captured from the wild by hormone injections. The experiments conducted on induced breeding of milkfish leading to the successful fertilization and hatching of milkfish eggs are briefly described. -
Decapsulation of Artemia cysts: A simple technique for the improvement of the use of brine shrimp in aquaculture
(Elsevier, 1977)Although it is a common practice in different disciplines of fundamental research on the brine shrimp, and despite the very interesting applications that it offers for the use of Artemia in aquaculture, the "decapsulation" technique, which removes the outer layer of the cyst shell of Artemia, is not known to shrimp and fish aquaculturists. The present paper describes the technology developed by the authors for the routine decapsulation of Artemia cysts. The advantages which result from the use of decapsulated cysts in aquacultural hatcheries are discussed.

















