Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff: Recent submissions
Now showing items 801-820 of 1693
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Partial replacement of Artemia sp. by the brackishwater cladoceran, Diaphanosoma celebensis (Stingelin), in the larval rearing of sea bass, Lates calcarifer (Bloch)
(Society of Israeli Aquaculture and Marine Biotechnology, 1998)A feed experiment was conducted to test the brackishwater cladoceran, Diaphanosoma celebensis, as a partial or complete substitute for Artemia in the larval rearing of the sea bass, Lates calcarifer. The cladoceran was fed either alone or in combination with Artemia to 15-day old sea bass larvae (5.6 mm SL, 2.7 mg wet BW) reared at 15 ppt salinity. Groups fed Artemia alone were reared at 15 and 32 ppt salinity levels. After 15 days of rearing, the survival (95-99%), observed mortality (0.8-1.2%) and apparent loss due to cannibalism (0.2-4.1%) did not differ significantly among treatments. The specific growth rates of fish fed only Artemia (18.7-19.1%/day) and combined Artemia+Diaphanosoma (18.7%/day) were significantly higher than that of fish fed only Diaphanosoma (16.3%/day). Diaphanosoma had higher crude protein and crude fat contents than Artemia but the percentage of n-3 highly unsaturated fatty acids, particularly 20:5n3 and 22:6n3, was lower than in Artermia. Results indicated the potential of Diaphanosoma as a partial substitute for Artemia in the larval rearing of sea bass at 15 ppt salinity. -
Effect of aquaculture on world fish supplies
(Nature Publishing Group, 2000)Global production of farmed fish and shellfish has more than doubled in the past 15 years. Many people believe that such growth relieves pressure on ocean fisheries, but the opposite is true for some types of aquaculture. Farming carnivorous species requires large inputs of wild fish for feed. Some aquaculture systems also reduce wild fish supplies through habitat modification, wild seedstock collection and other ecological impacts. On balance, global aquaculture production still adds to world fish supplies; however, if the growing aquaculture industry is to sustain its contribution to world fish supplies, it must reduce wild fish inputs in feed and adopt more ecologically sound management practices. -
Experimental infection of kuruma prawn (Penaeus japonicus) with Vibrio penaeicida
(The Society of Israeli Aquaculture and Marine Biotechnology, 1998)To estimate the portal of entry of Vibrio penaeicida in kuruma prawn (Penaeus japonicus), immersion and oral infection experiments were conducted with pre-treated and intact prawns. In immersion challenge, V. penaeicida produced 30% mortality in the shell-removed group at both 108 and 107 CFU/ml doses while in the eye-cut group, mortalities were 44% and 13% at 108 and 107 CFU/ml, respectively. In the intact group, 10% mortality was observed at 108 CFU/ml and no mortality at 107 CFU/ml after two weeks. The pathogen produced high mortalities in the orally incubated group, the median lethal dose (LD50) being 103-104 CFU/animal. In the groups given V. penaeicida-inoculated kuruma prawn meat and V. penaeicida-contaminated commercial pellets, mortalities within 60 days were 10% and 20%, respectively. These results suggest that the main portal of entry of V. penaeicida is the gastro-intestinal tract although the cuticle (epidermis) or wounds can also be portals. -
Thyroid hormones promote early metamorphosis in grouper (Epinephelus coioides) larvae
(Academic Press, 1998)The response of grouper larvae to the thyroid hormones, thyroxine (T4) and triiodothyronine (T3), was examined. Two-, 3-, and 4-week-old grouper larvae were reared in seawater containing either T4 or T3 at 0.01, 0.1, and 1 ppm. T4 or T3 induced metamorphosis in all age groups in a dose-dependent manner. Regardless of the size of the larvae, metamorphosis was completed in 2 days in larvae treated with 1 ppm of either T4 or T3; 3–4 days in larvae exposed to 0.1 ppm; and 5–6 days in larvae immersed in 0.01 ppm. None of the fish in the control group completed metamorphosis during this period. Compared with the control fish, survival rates were higher in groups exposed to 0.01 ppm and lower in those exposed to 1 ppm of T3. In 4-week-old larvae, T4 treatment (0.01 to 1.0 ppm) resulted in higher survival compared to the control. These results suggest that a dose of 0.01 ppm is appropriate for acceleration of metamorphosis and improvement of survival in 3- and 4-week-old grouper larvae. A lower dose may be apropriate for earlier stages. -
Studies on the reproductive biology of the Donkey's ear abalone, Haliotis asinina Linné
(Elsevier, 1998)The sex ratio, initial size at sexual maturity, spawning period, time interval between successive spawnings and fecundity of the Donkey's ear abalone, Haliotis asinina were studied. The sex ratio of wild abalone (>60 mm shell length) collected from Panagatan Cays, Antique, Philippines was approximately 1:1. Sexual maturity was first attained at a size of 35.0 mm and 35.9 mm for hatchery-reared male and female abalone, respectively, and 40.6 mm for wild-caught male and female specimens. Based on calculated gonad bulk indices and histological observations, wild-caught abalone appeared capable of spawning during most months of the study period except June for males and May–June for females, during which most specimens collected were at a resting stage. This abalone species is a serial spawner with an asynchronous spawning behavior. Hatchery-reared abalone have short time intervals between successive spawnings of 13–15 days. Ripe females measuring 58.1–69 mm in shell length may release about 150,000 to 600,000 eggs per spawning. -
Use of metaldehyde as a molluscicide in semi-commercial and commercial milkfish ponds
(Elsevier, 1998)The effect of metaldehyde on brackish water pond snails, Cerithidea cingulata, was tested in 250 m2 ponds, and in semi-commercial and commercial milkfish ponds. The field trials were conducted at three locations, namely Luzon, Visayas, and Mindanao, Philippines. Three application rates (80, 100, 120 kg(ha) of 10% metaldehyde formulation, an untreated control and a reference standard 25% niclosamide EC (1.0 L/ha) were tested during the dry season in a pond with heavy snail infestation. Seven days after application (DAA), snail mortality rates (86-87%) did not differ significantly among the various metaldehyde treatments but were significantly higher than those in the control (6%) and the reference standard (29%). In the field trials, in heavily infested ponds (more than 2000 snails/m2), a dose of 120 kg/ha was effective under both dry and wet conditions. In moderately infested ponds (less than 2000 snails/m2), a dose of 80 kg/ha of a 10% metaldehyde formulation was effective under dry conditions but a dose of up to 120 kg/ha was needed under wet conditions. In a pond dosed with 120 kg/ha of the 10% metaldehyde formulation, the concentration increased in pond water, until 3 DAA relative to the initial level, indicating that the active ingredient had dissolved from the granules. From day 3, the concentration declined steadily to approx. 16% of the initially detected amount at 15 DAA. In the pond sediment, the metaldehyde concentration steadily declined to approx. 1% of the initially detected amount at 15 DAA. As metaldehyde is rapidly degraded in aquatic systems its efficacy in controlling brackish water pond snails depends on a high initial dosage. -
Molluscicidal activity of tobacco dust against brackishwater pond snails (Cerithidea cingulata Gmelin)
(Elsevier, 1998)Toxicity tests on the effect of tobacco dust against brackishwater pond snails (Cerithidea cingulata Gmelin) of various stages or size ranges were conducted under controlled laboratory conditions. The 72-h LC50 for the juveniles, sub-adult and adult snails are 30, 87 and 166 kg ha−1, respectively, while the 72-h LC99 are 290, 522 and 712 kg ha−1, respectively. The nicotine content of the tobacco dust used was 2.8%. Lethal concentration increased as the life stages or size of the snail increased. Molluscicidal activity also progressively increased with time of exposure. The nicotine content and toxicity of different types of tobacco [flue-cured (neutral), flue-cured (full-flavor), burley and batek (full-flavor)] were determined on adult snails. Lethal concentrations (LC50) and (LC99) decreased as nicotine content of the tobacco dust increased indicating a higher molluscicidal activity. The effective application rate is affected by the nicotine content of the tobacco dust. An equivalent nicotine concentration of ca 24 kg ha−1 corresponded with the 72-h LC99 under laboratory conditions. Sevenday bioassay experiments on the effect of tobacco dust on two size groups of milkfish juveniles (0.5 and 2–3 g) showed no mortalities up to the concentration lethal to the pond snails. -
Fishing cooperatives in Capiz, central Philippines: their importance in managing fishery resources.
(Elsevier, 1998)Fishery cooperatives can co-manage coastal fishery resources, help improve the living conditions of small-scale fishers, and slow down the rapid depletion of these resources. Ten fisherfolk cooperatives in Capiz, central Philippines were assessed for their willingness to co-manage fishery resources. Seventeen members and three officers randomly selected from each of six mainland and four island-based cooperatives were interviewed using a pre-tested interview schedule. Statistical tools were employed to analyze field data. Specific factors related to origin and background, membership, management, and economic factors affect the success of cooperatives. Most cooperative members (74%) were willing to assume responsibility in managing fishery resources. Apathy and lack of advocacy and lobbying skills prevent the members from acquiring control and use rights over fishery resources. About 52% of them were aware of the 1991 Local Government Code and the provision on the participation of people's organization in local governance. -
Effect of salinity, dietary lipid source and level on growth of milkfish (Chanos chanos) fry
(Elsevier, 1998)Six semi-purified microparticulate diets containing coconut oil (CO), cod liver oil (CLO), and their 1:1 combination (CO+CLO) at 9% and 18% levels were fed to milkfish fry or late postlarvae in freshwater (0 ‰), brackishwater (16‰), and seawater (34‰) for 30 days. A three-factor factorial design (3×2×3) with three replicates per treatment was followed. Sixty-five milkfish fry (5 mg, 6 mm) were stocked per 15-l rectangular glass aquarium and fed with the experimental diets at 20% of biomass daily. Water temperature was 28±1°C during the culture period. Survival was not affected by water salinity, and lipid source or level. Among the 18 treatments, freshwater-reared milkfish fry fed with 9% CO+CLO had the highest specific growth rate, but this was not significantly different from those of freshwater-reared fish fed with 9–18% CO and 9% CLO diets or brackishwater-reared fish fed with 9% CO diet (P>0.05). As a main factor, salinity or dietary lipid level, but not lipid source, had significant effects on growth and feed conversion ratio of milkfish fry. Highest growth was observed in fish reared in freshwater and as salinity was increased, growth decreased (P<0.05). Overall, the 9% lipid diets promoted better growth than the 18% lipid diets (P<0.05). Except for the significant interaction between dietary lipid source and level indicating that milkfish fry fed with the 18% CLO was the shortest (P<0.05), no other significant effects of the two- or three-factor interactions were found. -
Culturing seahorse (Hippocampus barbouri) in illuminated cages with supplementary Acetes feeding
(The Society of Israeli Aquaculture and Marine Biotechnology (SIAMB), 2010)Juvenile Hippocampus barbouri were grown in illuminated cages with or without supplemental daytime feeding of thawed Acetes (a planktonic marine crustacean), or in non-illuminated cages with Acetes feeding, as a supplement to light-attracted zooplankton prey. After ten weeks, seahorses in illuminated cages fed Acetes had the highest mean body weight (2.24 g) and length (8.20 cm), but these did not significantly differ from seahorses in unfed illuminated cages (1.88 g; 7.25 cm), which did not significantly differ from those in fed non-illuminated cages (0.88 g; 6.32 cm). In all treatments, the mean instantaneous growth rate in body weight declined progressively throughout the test but the instantaneous growth rate in stretched length did not vary. Mean survival (76-100%) of seahorses in fed non-illuminated cages and in unfed illuminated cages did not vary significantly over the test period. The mean survival of seahorses in fed illuminated cages was lowest (54%), but did not significantly differ from the other treatments. Juvenile H. barbouri grown in illuminated cages had better growth than those in non-illuminated cages, but survival was reduced when seahorses in illuminated cages were fed Acetes. -
Diet development and evaluation for juvenile abalone, Haliotis asinina: protein/energy levels
(Elsevier, 1999)Juveniles of abalone, Haliotis asinina with mean initial weight and shell length of 0.6±0.03 g and 15±0.07 mm were fed practical diets for 90 days. The practical diets contained graded levels of protein from fish meal, shrimp meal, and soybean meal at 22 (diet 1), 27 (diet 2), and 32% (diet 3) with corresponding estimated metabolizable energy values of 3254, 3150, and 3090 kcal ME/kg diet. The amino acid profile and proximate analyses of muscle meat of the test animal and published nutrient requirements of other species of Haliotids were used as a basis for formulating and developing these practical diets. The diets were fed to abalone at 2–5% body weight once daily (1600 h) for biological evaluation in terms of weight gain (WG), increase in shell length (SL), specific growth rate (SGR), protein efficiency ratio (PER), feed conversion ratio (FCR) and for physical evaluation in terms of shell coloration of the animal. Natural food, Gracilariopsis bailinae (17% CP/2200 kcal ME/kg) (NF) fed ad libitum served as the control. Better growth rates, in terms of WG and SL, FCR and PER were noted in abalone fed the three formulated diets compared with those fed seaweed, G. bailinae. Abalone fed diets 3 (WG: 347%; SGR: 0.81; SL: 140%) and 2 (WG: 307%; SGR: 0.70; SL: 139%) showed significantly better growth rate than those fed diet 1 (WG: 252%; SGR: 0.51; SL: 132%). Natural food-fed abalone had the lowest WG (134%), SGR (0.06) and increase in SL (77%). Survival was generally high at 85–95% for all treatments. FCR (1.5–2.3) and PER (2.19–2.47) for animals fed the three diets were significantly better than for those fed natural food (0.10 PER and 6.98 FCR). Regression analysis showed the optimum protein level for juvenile abalone to be 27% with an energy level of 3150 kcal/kg ME. Abalone juveniles fed the formulated diets produced shells with light bluish green color while those fed seaweed retained the original brown color. Diet 2 which contained 27% protein, 5% lipid and 40% carbohydrates with an energy value of 3150 kcal/kg ME may be used as a basal diet for the rearing of juvenile abalone, H. asinina. -
Nature parks, museums, gardens, and zoos for biodiversity conservation and environment education: the Philippines
(Springer Verlag, 1998)Public consciousness about biodiversity and the environment, and their importance for sustainable development is not widespread in the Philippines. This article advocates nonformal environment education through nature recreation as a means toward 'greening, the mind and the spirit of the citizens. Information is provided about biodiversity, and the status and potential of nature parks, museums, gardens, and zoos in the country. Many of the 116 national parks and protected areas have been exploited for products and energy, and only some provide for recreation-cum-education. The Philippines has no national botanical garden, zoo, or aquarium, and the National Museum is not the proud institution that it should be. Some universities have small museums, botanical gardens, and other biodiversity exhibits for instruction and research, but these and the few zoos and wildlife centers are poorly funded or managed. -
Dietary onion and ginger enhance growth, hemato-immunological responses, and disease resistance in brown-marbled grouper, Epinephelus fuscoguttatus
(Bioflux, 2012)A 12-week (September to December 2009) feeding trial was conducted to evaluate theimmunostimulatory effects of different substances administered orally through the diet in the brown-marbled grouper, Epinephelus fuscoguttatus. Five experimental diets containing either onion, ginger, β–glucan, or vitamin C and a control diet (without immunostimulants) were fed to the fish weighing about 44 g for 12 weeks. Onion-fed fish showed significantly increased weight gain, hematocrit, and total Ig compared to the control group; however, leukocyte differential count and ROS production were unaffected. Ginger-fed fish likewise significantly increased total Ig, ROS production and lysozyme activity. However, it did not affect growth and hematocrit value. β-glucan significantly increased growth and total Ig but had no effect on the other parameters. Vitamin C significantly increased hematocrit, total Ig and ROS production but did not increase growth. Upon challenge with a bacterial pathogen Vibrio harveyi, mortality was significantly reduced in the onion, ginger and vitamin C-fed fish but not in the β–glucan-fed fish. This study demonstrated that onion and ginger could positively affect the innate immune responses and protect grouper against Vibrio harveyi infection. -
Optimization of culture conditions for tissue culture production of young plantlets of carrageenophyte Kappaphycus
(Springer Verlag, 2011)To improve the production of Kappaphycus plantlets in tissue culture, optimum media concentrations of an Ascophyllum nodosum extract (Acadian Marine Plant Extract Powder, AMPEP), plant growth regulators (PGR), pH–temperature combinations, and explant density were determined. Kappaphycus alvarezii var. tambalang purple (PUR), kapilaran brown (KAP), vanguard brown (VAN), adik-adik (AA), tungawan green (TGR), and K. striatum var. sacol green (GS) were used as explants. Based on the shortest period for shoot emergence and the economical use of AMPEP, the optimum enriched media was 3.0 mg L−1 AMPEP and 0.1 mg L−1 AMPEP + PGR 1 mg L−1 each phenylacetic acid (PAA) and zeatin for PUR, 1.0 mg L−1 AMPEP + PGR for KAP and GS, 0.1 mg L−1 AMPEP + PGR for VAN, and 3.0 mg L−1 AMPEP and 0.001 mg L−1 AMPEP + PGR for AA and TGR. Results showed that the addition of PGR to low concentrations of AMPEP hastened shoot formation. pH–temperature combinations for the most rapid shoot formation were determined for the brown (KAP) and purple (PUR) color morphotypes of K. alvarezii var. tambalang and the green morphotype of K. striatum var. sacol (GS) cultured in 1.0 mg L−1 AMPEP + PGR. The brown morphotype produced the most number of shoots at pH 7.7 at 20°C after as little as 20 days. Purple K. alvarezii showed an increased shoot formation at pH 6.7 at 25°C and the green K. striatum morphotype at pH 8.7 at 25°C. The optimum number of explants added to the culture media was also determined for tungawan green (TGR), brown (KAP), and tambalang purple (PUR) varieties of K. alvarezii in 1.0 mg L−1 AMPEP + PGR. The number of explants and the volume of the culture media combination were also tested. The highest average number of shoots formed occurred in two explants:1 mL culture media (2:1) for KAP and PUR (35.00% and 16.67%, respectively) and 1 explant: 2 mL culture media for the TGR (100.00%) with a range of 0.5–3.0 mm shoot length after 40 days in culture. The earliest shoot formation was observed after 21 days for the brown and 9 days for both the green and purple color morphotypes of Kappaphycus, in all densities investigated. This indicated that within the range tested, the density of explants did not have a significant effect on the rate of shoot formation but did influence the average number generated from the culture. The rate of production of new and improved Kappaphycus explants for a commercial nursery stock was improved through the use of AMPEP with optimized culture media pH, temperature, and density conditions. -
Identification of Pseudomonas sp. strain S3 based on small subunit ribosomal RNA gene sequences.
(Asian Network for Scientific Information, 2010)Pseudomonas sp. strain S3 was isolated from Paddy (rice) field agricultural area. This organism, which can utilize a halogenated compound of D,L-2-Chloropropionic acid as sole carbon and energy source, catalyses the hydrolytic dehalogenation of both D- and L- isomers of 2-Chloropropionic acid. Identification of Pseudomonas sp. S3 is still ambiguous due to the lack of basic studies, especially their molecular genetic information. In this study, the amplified 16S rRNA gene sequence of Pseudomonas sp. S3 (Accession No. FJ968758) was compared to other nine selected gene sequences from the same group of Pseudomonas sp. and/or dehalogenase producing bacteria using in silico method. Their phylogenetic relationships were then determined. The results were analysed using MEGA4 software to ascertain its evolutionary distance by reconstructing a phylogenetic tree of these organisms. The evolutionary history and bootstrap consensus tree were inferred using the Neighbour-Joining method from 500 replicates. The tree is drawn to scale, with branch lengths (next to the branches) in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the p-distance method and were in the units of the number of base substitutions per site. Based on this analysis, Pseudomonas sp. S3 16S rRNA gene was closely related to the Pseudomonas chlororaphis with genetic distance 0.170 base substitutions per site. S3 gene was also compared among known dehalogenase producing bacteria 16S rRNA genes. Results suggested that S3 was closely related to the Pseudomonas sp. R1 with a genetic distance 0.040 base substitutions per site. From present study, evolutionary relationships of 16S rRNA gene of Pseudomonas sp. S3 were elegantly illustrated by phylograms, comparable to a pedigree showing which microorganisms are most closely related. -
Culture of Macrobrachium rosenbergii (De Man 1879) in experimental cages in a freshwater eutrophic lake at different stocking densities
(Blackwell Publishing, 2007)Macrobrachium rosenbergii (de Man 1879) juveniles (0.4 g) were cultured in experimental cages (L × W × H: 2.5 × 1 × 1 m) in Laguna de Bay, the largest lake in the Philippines. The following stocking densities at four replicates each were used: 15, 30, 60 and 90 prawns m−2 of cage bottom. The mean sizes at harvest after 5 months of culture ranged from 14.3 g for the highest stocking density to 26.3 g for the lowest. The mean size at harvest, daily growth rate and size class distribution were significantly influenced by stocking density, with those at the lowest stocking density showing significantly better growth and overall proportion of larger prawns. Heterogeneous individual growth (HIG) was fairly evident in all treatments. The percentage of blue-clawed males was not influenced by treatment but the mean weight was significantly higher in the lower stocking densities. Both the percentage and mean weight of berried females were significantly higher in the lowest stocking density. Survival was the highest in the lower stocking densities (55.3%, 54.0%, 52.7% and 36.9% for 15, 30, 60 and 90 prawns m−2 respectively). Feed conversion ratio (FCR) improved with decreasing stocking density, ranging from 2.1 to 3. As expected, yield per cropping increased with stocking density and ranged from 450 to 1089 g m−2 yr−1 of actual cage area. Production values obtained in the cage cultured M. rosenbergii were comparable to or even higher than those reported from pond culture, given that the stocking densities used in this study were generally higher than in ponds. The results show that the farming of M. rosenbergii in cages in lakes is a viable alternative to pond culture and has the potential of improve aquaculture production in lakeshore fish farming communities. -
Antibacterial activity of tilapia Tilapia hornorum against Vibrio harveyi
(Elsevier, 2004)Disease due to luminous Vibrio has been a major problem of the shrimp industry. Different technologies have been introduced to control the disease. One of the techniques reported to work against luminous bacteria in the Philippines is the green water culture system (or finfish–shrimp integrated culture system). A green water culture system is an innovative technique wherein shrimp are cultured in water collected from a pond where tilapia or other fish species are grown. In some cases, the fish are cultured in an isolated net pen inside the shrimp culture pond. This study clarifies the effect of one component of the green water culture system, the presence of all male tilapia (Tilapia hornorum) on luminous bacteria Vibrio harveyi. Results showed that stocking tilapia at a biomass not lower than 300 g/m3 efficiently inhibited the growth of luminous bacteria in shrimp (biomass=80 g/m3) rearing water without the growth of microalgae. -
Propagule production of Eucheuma denticulatum (Burman) Collins et Harvey by tissue culture.
(Walter de Gruyter, 2003)Plants of Eucheuma denticulatum collected from Danajon Reef, Bohol, the Philippines were used to produce tissue cultures for the purpose of generating new strains. The plants were sectioned and grown in media encouraging callus growth. New shoots were produced after 3 months of in vitro culture. After another 3 months of laboratory growth, 5 plants were outplanted in the field. After 4 months growth at approximately 0.50 m depth, the five plants grew to biomasses that ranged from 354.9 to 1055.9 g wet weight. They demonstrated growth rates that ranged from 5.8 to 7.2% day−1. The growth rates of the plants produced through tissue culture in this study suggest that such plants can grow at rates as good as, if not better than, those of 'seedlings' produced by the traditional method in the commercial cultivation of E. denticulatum. -
mRNA expression patterns for GH, PRL, SL, IGF-I and IGF-II during altered feeding status in rabbitfish, Siganus guttatus.
(Elsevier, 2007)Feeding time is a major synchronizer of many physiological rhythms in many organisms. Alteration in the nutritional status, specifically fasting, also affects the secretion rhythms of growth hormone (GH) and insulin-like growth factor-I (IGF-I). In this study, we investigated whether the expression patterns for the mRNAs of GH, prolactin (PRL) and somatolactin (SL) in the pituitary gland, and insulin-like growth factor I and II (IGF-I and IGF-II) in the liver of juvenile rabbitfish (Siganus guttatus) follow a rhythm according to feeding time and whether these hormone rhythms changes with starvation. Hormone mRNA levels were determined by real time PCR. The daily expression pattern for the mRNAs of GH, PRL and SL was not altered whether food was given in the morning (10:00 h) or in the afternoon (15:00 h). The daily GH mRNA expression pattern, however, was affected when food was not available for 3 days. In contrast, the daily expression pattern for IGF-I mRNA reaches its peak at roughly 5–6 h after feeding. This pattern, however, was not observed with IGF-II mRNA. During 15-day starvation, GH mRNA levels in starved fish were significantly higher than the control fish starting on the 9th day of starvation until day 15. The levels returned to normal after re-feeding. In contrast to GH, PRL mRNA levels in starved fish were significantly lower than the control group starting on the 6th day of starvation until 3 days after re-feeding. SL mRNA levels were not significantly different between the control and starved group at anytime during the experiment. Both IGF-I and IGF-II mRNA levels in starved group were significantly higher than the control fish on the 3rd and 6th day of starvation. mRNA levels of both IGF-I and II in the starved fish decreased starting on the 9th day of starvation. While IGF-I mRNA levels in the starved group continued to decrease as starvation progressed, IGF-II mRNA levels were not significantly different from the control during the rest of the starvation period. The results indicate that aside from GH and IGF-I, PRL and IGF-II are likewise involved in starvation in rabbitfish. -
Evaluation of mucus, Navicula, and mixed diatoms as larval settlement inducers for the tropical abalone Haliotis asinina
(Elsevier, 2003)Different biological films reportedly effective in larval settlement of other abalone species were tested on the tropical abalone Haliotis asinina to determine the most effective settlement inducers for the species. Larval attachment was significantly higher on mucus, Navicula, Navicula+mucus, and mixed diatoms+mucus than on mixed diatoms and the blank control. Metamorphosis was significantly higher on Navicula, Navicula+mucus. These results indicate the important contribution of mucus in larval settlement and the effectiveness of Navicula as a single species over mixed diatoms in larval settlement of the tropical abalone H. asinina.





















