Growth response and carcass composition of red tilapia fry fed diets with varying protein levels and protein to energy ratios
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An 8-week feeding experiment was conducted with red tilapia (Oreochromis ) fry of 0.160 plus or minus 0.035 g initial weight. Twelve diets of 4 protein levels (25, 30, 35 and 40%) and 3 protein to energy (P/E) ratios (111, 100 and 80 mg protein/kcal) at each protein level were used. The highest growth was attained by fry fed a 40% protein diet with a P/E ratio of 111 mg/kcal. A lower but not a significantly different growth response was attained by fry on a 35% protein diet with a P/E ratio of 111 mg/kcal and a 30% protein diet with a P/E ratio of 100. Protein efficiency ratio was affected by the dietary protein level. Feed conversion ratio was not significantly influenced by the dietary protein level nor the P/E ratio. Carcass moisture content (%) was affected only by the P/E ratio. Carcass moisture content was directly related to the P/E ratio of the diets and was inversely related to the digestible energy (DE) level. Both protein level and P/E ratio significantly influenced carcass lipid content (%) on a dry matter basis but not the ash content. Carcass lipid (5) increased with increasing dietary protein and increasing DE levels of the diet, but decreased with increasing P/E ratio. Carcass protein content decreased significantly with the decrease of P/E ratio and increase of DE level of the diet.
Santiago, C. B., & Laron, M. A. (1991). Growth response and carcass composition of red tilapia fry fed diets with varying protein levels and protein to energy ratios. In S. S. De Silva (Ed.), Fish Nutrition Research in Asia. Proceedings of the Fourth Asian Fish Nutrition Workshop, 3-8 September 1990, Vijayawada, Andhra Pradesh, India (pp. 55–62). Manila, Philippines: Asian Fisheries Society.
PublisherAsian Fisheries Society
- Conference Proceedings 
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Evaluation of dietary freeze-dried Chaetoceros calcitrans supplementation to control Vibrio harveyi infection on Penaeus monodon juvenile Effects of supplementation of diets with freeze-dried Chaetoceros calcitrans to control Vibrio harveyi infection are evaluated through immune responses, and disease resistance of juvenile Penaeus monodon. Total lipid and fatty acid profile of Chaetoceros calcitrans is also analyzed. A challenge infection with 107 cfu/mL concentration of Vibrio harveyi is intramuscularly injected to juvenile Penaeus monodon after 45 days of feeding of diets supplemented with 15 g/kg and 30 g/kg dried Chaetoceros calcitrans. The use of dried Chaetoceros calcitrans is compared with that of ß-1,3 glucan Curdlan, a commercial immune enhancer. Incorporation of 30 g/kg Chaetoceros calcitrans in the diet enhances the immune system of shrimp as effected by high prophenoloxidase activity and plasma protein concentration and is better compared to the commercially available Curdlan. Chaetoceros calcitrans also contains polyunsaturated fatty acids (PUFAs) such as linolenic acid and eicosapentanoic acid (EPA) which are responsible for its antibacterial action against Vibrio harveyi. All these biological activities of Chaetoceros calcitrans add up to increase resistance of the juvenile Penaeus monodon to vibriosis as shown by its high survival rate from the challenge infection with Vibrio harveyi. Therefore, it is worthwhile to use Chaetoceros calcitrans as supplementary feed. Its effect in increasing the immune competence coupled with its antibacterial action, make the shrimp resistant to luminous vibriosis that continues to affect the industry, thereby augmenting aquaculture production.
Molecular cloning and localization of GABAA receptor-associated protein in the rotifer Brachionus plicatilis HS Marcial, K Suga, S Kinoshita, G Kaneko, A Hagiwara & S Watabe -
International Review of Hydrobiology, 2014 - Wiley-VCH Verlagγ-Aminobutyric acid receptor type A-associated protein (GABARAP) and its homologs constitute a protein family found in many eukaryotes from yeast to human, and are known to be involved in intracellular membrane trafficking of GABAA receptors and autophagy. In this study, we cloned cDNA-encoding GABARAP from the monogonont rotifer Brachionus plicatilis and examined for its tissue distribution at the protein level in neonates, males and females. Using reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE) techniques, we showed that like other GABARAPs, rotifer GABARAP was also composed of 117 amino acids and highly homologous to vertebrate GABARAP2 ortholog (74–76% identity). GABARAP was demonstrated with its specific antibody to be ubiquitously distributed, irrespective of neonates, males, and females, in the coronal area that covers brain and contains most mechano- and chemoreceptors. Rotifer GABARAP was also expressed in the mature eggs but not in immature eggs. Double immunostaining with mammalian anti-GABA γ receptor antibody showed that rotifer GABARAP co-localized with GABA receptor, suggesting the association of the two proteins. The presence of GABARAP in rotifer implies that it is highly conserved during evolution, and plays important roles in various biological processes.
Conference paperRM Coloso - In MR Catacutan, RM Coloso & BO Acosta (Eds.), Development and Use of Alternative Dietary Ingredients or Fish Meal Substitutes in Aquaculture Feed Formulation … Ingredients or Fish Meal Substitutes in Aquaculture Feed Formulation, 9-11 December 2014, Nay Pyi Taw, Myanmar, 2015 - Aquaculture Department, Southeast Asian Fisheries Development CenterFish production from aquaculture in Asia has steadily increased during the past decade. In 2012, Asia s share in the total world aquaculture production was about 89% with 60 M metric tons valued at US$ 120 B. ASEAN Member States such as Viet Nam, Indonesia, Thailand, Myanmar, and the Philippines are among the top producers in Asia contributing 9 M metric tons of production from aquaculture valued at US$ 19 B (FAO, 2014). To sustain the production and profitability of aquaculture operations, reducing costs is needed mainly through feeds and feeding which represent up to 60% of operational costs. Reductions in feeding costs can be realized through optimizing nutrient levels of diets, feeding strategies, and by using plant protein sources as fish meal substitutes. As more intensive methods for production of the top five commodities (carps, tilapia, milkfish, catfish, and Pangasius sp.) become popular in ASEAN Member States, practical feeds need to be formulated using plant protein sources that are locally available. Plant protein sources such as soy proteins and corn gluten have been used as partial or total replacements for fish meal quite extensively in aquafeed for the top aquaculture commodities because of their high protein content (40-60%) and good digestibility. Other alternative dietary protein sources with emphasis on oilseed meals, peas and other leguminous seed meals, leaf meals from terrestrial plants, aquatic plants, plant protein concentrates, single cell proteins, cereal by products, fermentation and other products have been or are currently being evaluated as fish meal substitutes for their nutritive values, inclusion levels, constraints in processing mainly to reduce the effects of anti-nutritional factors as well as economic value. The proper use of these ingredients would promote good fish growth, survival, production, and boost the income of small scale farmers. Testing of aquaculture feeds containing these local ingredients will help the regional as well as worldwide research and development efforts and ultimately benefit the local small scale fish farmers and other stakeholders.