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Reproduction, early development, and larval rearing of the climbing perch Anabas testudineus (Teleostei: Anabantidae) in captivity
(College of Agriculture and Food Science, University of the Philippines Los Baños, 2023-06)
Induced spawning, early development, and larval rearing of the climbing perch Anabas testudineus were examined under captive conditions. Five female climbing perch [125.33 ± 3.27 mm standard length (SL); 70.67 ± 5.59 g body weight (BW)] were each paired with apparently mature males (108.50 ± 3.97 mm SL; 39.27 ± 4.70 g BW) and induced to spawn with 5,000 IU human chorionic gonadotropin (hCG) + 0.5 mL Ovaprim/kg BW. All hCG + Ovaprim-injected fish spawned 9 – 11 h after hormone administration but saline-injected fish (control group) failed to spawn. Mean egg production per female, fertilization and hatching rates, and larval production per female were 7,667 ± 1,313, 98.11 ± 1.63%, 61.01 ± 19.92%, and 4,435 ± 1,041, respectively. Fertilized eggs (1.52 – 1.96 mm in diameter) were almost spherical, non-adhesive, transparent, and buoyant due to a large, single oil globule (515 ± 33 µm in diameter). First cleavage appeared 30 min post-fertilization (MPF) and egg development lasted for 20 – 24 h post-fertilization (HPF) (29°C). Newly hatched climbing perch larvae [1.73 ± 0.08 mm in total length (TL)] with yolk volume (YV) and oil globule volume (OGV) of 0.545 ± 0.104 mm<sup>3</sup> and 0.072 ± 0.013 mm<sup>3</sup>, respectively had no pigmented eyes or functional mouth parts and digestive tract, and immobile with the yolk sac pointed upward. Eyes became pigmented and both mouth and anus opened in 1 d old larvae (2.09 ± 0.04 mm TL) in preparation for exogenous feeding. Except for body depth (BD) and pre-anal length (PAL), a general increase in TL (1.73 – 3.72 mm), eye diameter (ED) (0.164 – 0.378 mm), head length (HL) (0.205 – 0.923 mm), and mouth gape (MG) size (0.308 – 0.552 mm) was observed, coinciding with yolk resorption in 7 d post-hatched larvae. Climbing perch larvae were reared at four stocking densities (25, 50, 75, 100 larvae L-1) and fed solely on live food (Brachionus rotundiformis from days 2 to 12 and Artemia nauplii from days 13 to 40) or co-fed live food and microparticulate diet (MPD) (Artemia nauplii from days 2 to 15 and Artemia + MPD from days 16 to 40). Mean survival rates at lower densities (25 – 50 larvae L<sup>-1</sup>) were significantly higher than those reared at 75 and 100 larvae L<sup>-1</sup> (P < 0.05). Furthermore, climbing perch larvae co-fed Artemia nauplii and MPD showed better survival (28.67 – 81.00%) than those fed solely on live food (24.88 – 64.00%). However, at the end of the 40 d trial period, growth parameters did not vary significantly among densities (P > 0.05), except for the condition factor in larvae co-fed Artemia nauplii and MPD. Taken together, results demonstrate the effectiveness of hCG + Ovaprim for captive breeding and observations on the early development of climbing perch, and the feasibility of rearing the larvae at 25 – 50 L<sup>-1</sup> on a combination of Artemia nauplii and MPD under laboratory conditions....
High amorphous poly-beta-hydroxybutyrate (PHB) content in a probiotic Bacillus strain displays better protective effects in Vibrio-challenged gnotobiotic Artemia
(Elsevier, 2018)
In this study, the Bacillus sp. JL47, a superior PHB-accumulating Bacillus strain identified from the previous work, was tested for its protective effects in gnotobiotic Artemia franciscana during a pathogenic Vibrio ...
Evaluation of various leguminous seeds as protein sources for milkfish, Chanos chanos Forsskal, juveniles
(Asian Fisheries Society, 1987)
The nutritive value of four leguminous plant seeds: pigeonpea (Cajanus cajan), mungo (Phaseolus radiatus), kidneybean (Phaseolus vulgaris) and soybean (Glycine max), were evaluated as protein sources for milkfish (Chanos ...
Boosting the sustainability of aquaculture feed for the lucrative business of mangrove crab grow-out culture
(Secretariat, Southeast Asian Fisheries Development Center, 2023-12)
Conventional feeds for mangrove crab (Scylla serrata) are highly fixated on the use of fish-by-products (FBC) and other slaughter wastes. These feeds, however, are highly polluting that can easily cause water quality ...
Use of rotifers-fed microalgal paste in the seed production of mangrove crab Scylla serrata in the Philippines
(Springer, 2022-09-09)
Despite the progress in the production of artificial diets for marine larvae, feeding during the early life stages of most aquaculture species still relies on live feeds such as rotifers. Advanced rotifer culture techniques ...
Simulated transport of silver therapon (Leiopotherapon plumbeus) fingerlings at different durations and loading densities: Effects on survival and water quality
(College of Agriculture and Food Science, University of the Philippines Los Baños, 2024-06)
The silver therapon, Leiopotherapon plumbeus, is being promoted as a new species for aquaculture. However, information on the optimal conditions for the transport of this species for grow-out culture needs to be established ...