Progress and current status of diagnostic techniques for marine fish viral diseases at the SEAFDEC Aquaculture Department.
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The incidence of unexplained mortalities among marine finfish in the Philippines has been increasingly observed. Considering that outbreaks of viral infections affecting similarly cultured marine fishes such as grouper and seabass were reported in many countries, a comprehensive diagnostic program to meet the challenge was initiated at the Aquaculture Department, Southeast Asian Fisheries Development Center (SEAFDEC-AQD)with funding from the Japanese Trust Fund Fish Disease Project. This activity was further boosted by the Japan International Research Center for Agricultural Sciences (JIRCAS). Overall, the program involved the staff of the marine finfish hatchery and the Fish Health Section. Cases of unexplained mortalities observed in the hatchery were referred to the Fish Health Section. Detailed information on the culture histories of each case were provided by the hatchery staff. Diagnostic tests were performed on each case and those with potential indication of viral etiology were processed for virus detection. Presumptive diagnosis of viral infections was based on typical signs, cell culture isolation histopathology and in-vivo pathogenicity tests. Confirmatory tests to identify specific viruses include RT-PCR, FAT and electron microscopy. The highlights of outbreaks of viral nervous necrosis and other virus-associated infections among marine finfish at SEAFDEC-AQD are presented.
Lio-Po, G. D., Cruz-Lacierda, E. R., de la Peña, L. D., Maeno, Y., & Inui, Y. (2002). Progress and current status of diagnostic techniques for marine fish viral diseases at the SEAFDEC Aquaculture Department. In Y. Inui & E. R. Cruz-Lacierda (Eds.), Disease Control in Fish and Shrimp Aquaculture in Southeast Asia – Diagnosis and Husbandry Techniques: Proceedings of the SEAFDEC-OIE Seminar-Workshop on Disease Control in Fish and Shrimp Aquaculture in Southeast Asia – Diagnosis and Husbandry Techniques, 4-6 December 2001, Iloilo City, Philippines (pp. 97–106). Tigbauan, Iloilo, Philippines: SEAFDEC Aquaculture Department.
PublisherAquaculture Department, Southeast Asian Fisheries Development Center
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Book chapterGD Lio-Po & LHS Lim - In PTK Woo, DW Bruno & LHS Lim (Eds.), Diseases and disorders of finfish in cage culture, 2014 - CABI PublishingThis chapter presents the viral, bacterial, pseudofungal and parasitic diseases in cultured warm freshwater fish. Focus is given on the distribution, causative agent, pathology, diagnosis, prevention and control of these diseases.
BookA textbook on diseases of cultured warmwater fish and shrimps in the Philippines. Eleven chapters cover essential information on the basic principles of disease causation, major diseases of cultured fish and crustaceans, particularly shrimps, and methods of prevention and control. Emphasis is made on major diseases that occur in the Philippines and other countries in the Asian region. Included also are topics on harmful algae, immunology and molecular biological diagnostic techniques.
BookCR Lavilla-Pitogo, GD Lio-Po, ER Cruz-Lacierda, EV Alapide-Tendencia & LD de la Peña - 2000 - Aquaculture Department, Southeast Asian Fisheries Development Center
Series: Aquaculture extension manual / SEAFDEC. Aquaculture Department; No. 16The manual provides information on the diseases that affect the 3 major species of shrimps cultured in the Philippines: Penaeus monodon, P. merguiensis and P. indicus. It includes the common name of the disease, causative agent, species affected, stages affected, gross signs, effects on the host and methods of prevention and treatment. This revised edition includes newly discovered diseases. It is hoped that the manual will be of considerable help to shrimp farmers in identifying the disease and lead to prevention or early disease diagnosis and control.