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Standardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon)

dc.contributor.authorde la Peña, Leobert D.
dc.contributor.authorLavilla-Pitogo, Celia R.
dc.contributor.authorPaner, Milagros G.
dc.contributor.editorNagasawa, Kazuya
dc.date.accessioned2020-07-21T00:31:42Z
dc.date.available2020-07-21T00:31:42Z
dc.date.issued2005-03
dc.identifier.citationde la Peña, L. D., Lavilla-Pitogo, C. R., & Paner, M. G. (2005). Standardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon). In K. Nagasawa (Ed.), Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia (pp. 19–26). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.en
dc.identifier.isbn9718511732
dc.identifier.urihttp://hdl.handle.net/10862/5907
dc.description.abstractSeveral runs were conducted using the three published PCR protocols and the three DNA extraction methods. All three primer pairs designed by individual authors based on the sequence of a cloned fragment of the white spot syndrome virus (WSSV) genome successfully detected the Philippine WSSV isolates. In addition, a high specificity of the assay was also obtained. CTAB and DNAzol extraction methods consistently gave a better DNA quality using the three PCR protocols. Any of the three PCR protocols can be used in the rapid and sensitive detection of WSSV in shrimp (Penaeus monodon) and crab (Episesarma vesicolor).en
dc.language.isoenen
dc.publisherAquaculture Department, Southeast Asian Fisheries Development Centeren
dc.subjectPenaeus monodonen
dc.subjectEpisesarma versicoloren
dc.subjectPenaeus japonicusen
dc.titleStandardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon)en
dc.typeBook chapteren
dc.citation.spage19en
dc.citation.epage26en
dc.citation.bookTitleRecent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asiaen
dc.subject.asfaWhite spot syndrome virusen
dc.subject.asfastandardizingen
dc.subject.asfapolymerase chain reactionen
dc.subject.asfadisease recognitionen
dc.subject.asfaanimal diseasesen
dc.subject.asfaDNAen
dc.subject.asfafreshwater crustaceansen
dc.subject.asfashrimp cultureen
dc.subject.asfaRNAen
dc.subject.scientificNamePenaeus monodonen


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