SEAFDEC/AQDINSTITUTIONAL REPOSITORY
    • Login
    View Item 
    •   SEAFDEC/AQD Institutional Repository Home
    • 02 SEAFDEC/AQD Collaborative Publications
    • SEAFDEC/AQD-Government of Japan-Trust Fund (GOJ-TF)
    • Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia
    • View Item
    •   SEAFDEC/AQD Institutional Repository Home
    • 02 SEAFDEC/AQD Collaborative Publications
    • SEAFDEC/AQD-Government of Japan-Trust Fund (GOJ-TF)
    • Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Standardization of diagnostic methods for monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV): Establishment of monoclonal antibodies (MAbs) against MBV and HPV

    • Global styles
    • APA
    • Elsevier - Harvard
    • Vancouver
    • MLA
    • Help
    Thumbnail
    View/Open
    CatapES2005.pdf (158.1Kb) AQD Access AQD Access
    Downloads: 2
    Date
    2005-03
    Author
    Catap, Elena S.
    de la Peña, Leobert D.
    Page views
    676
    Metadata
    Show full item record
    
    Share 
     
    Abstract
    Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) remain as two of the serious pathogens that infect black tiger shrimp (Penaeus monodon) in the Philippines. It is, therefore, necessary to prevent the spread and occurrence of such pathogens, especially in shrimp hatcheries where postlarvae (PLs) stocked in culture ponds are usually obtained. One effective means is to establish rapid and reliable diagnostic techniques for these viruses.

    This research was undertaken mainly to develop monoclonal antibodies (MAbs) against MBV and HPV. Consequently, these MAbs will be used as primary reagents to establish an appropriate immunologically-based detection method for MBV and HPV. Immunologically-based diagnostic methods have proven to be rapid, sensitive and quite specific. Furthermore, they could be performed in the field using very simple set-up.

    Four main studies were conducted for this research. The first involves the development of infection model of HPV in P. monodon. Series of experiments showed that HPV could be horizontally-transmitted in postlarval P. monodon through the oral route. This allowed the availability of infected materials to be used as immunogen and for screening of antibodies. The infection model also provided a method by which the pathogen could be further studied, including its control in hatchery conditions. The second study describes the development of MAbs against HPV and MBV, including the optimization of an immunofluourescent antibody test (IFAT), as a screening technique. The third study involves the production of polyclonal antibodies (PAbs) against MBV and HPV wherein IFAT, immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA) were optimized and used to screen the antibodies as to their usefulness in MBV and HPV diagnosis. The fourth set of experiments were undertaken to determine the efficiency of chlorine as a disinfectant in inhibiting HPV infection in P. monodon PLs. The results of each study are discussed in this report.
    URI
    http://hdl.handle.net/10862/5908
    Suggested Citation
    Catap, E. S., & de la Peña, L. D. (2005). Standardization of diagnostic methods for monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV): Establishment of monoclonal antibodies (MAbs) against MBV and HPV. In K. Nagasawa (Ed.), Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia (pp. 27–28). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
    Type
    Book chapter
    ISBN
    9718511732
    Subject
    Monoclonal antibodies; Disease detection; Animal diseases; Enzyme-linked immunosorbent assay; Baculovirus; ELISA; Penaeus monodon; Polyclonal antibodies
    Collections
    • Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia [43]


    © SEAFDEC/AQD  2021
    Contact Us | Send Feedback
     

     

    Browse

    All of SAIRCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsKeywordsThis CollectionBy Issue DateAuthorsTitlesSubjectsKeywords

    My Account

    LoginRegister

    Statistics

    View Usage Statistics
    SEAFDEC/AQD Library

    © SEAFDEC/AQD  2021
    Contact Us | Send Feedback
     

     

    Export citations

    Export the current results of the search query as a citation list. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

    The list of citations that can be exported is limited to items.

    Export citations

    Export the current item as a citation. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

    Export Citations

    EXTERNAL LINKS DISCLAIMER

    This link is being provided as a convenience and for informational purposes only. SEAFDEC/AQD bears no responsibility for the accuracy, legality or content of the external site or for that of subsequent links. Contact the external site for answers to questions regarding its content.

    If you come across any external links that don't work, we would be grateful if you could report them to the repository administrators.

    Click DOWNLOAD to open/view the file. Request a copy in case the link we provided don't work.

    Download

    DOCUMENT REQUEST NOT AVAILABLE

    This publication is still available (in PRINT) and for sale at AQD bookstore. The library is currently restricted to send PDF of publications that are still for sale.

    You may contact bookstore@seafdec.org.ph or visit AQD bookstore for orders.

    FILE UNDER EMBARGO

    This file associated with this publication is currently under embargo. This will be available for download after the embargo date.