Development of shrimp (Penaeus monodon) cell culture in vitro
MetadataShow full item record
Primary shrimp cell cultures were successfully developed from lymphoid organ of black tiger shrimp (Penaeus monodon) in double-strength Leibovitz’s L-15 medium supplemented with 15% fetal bovine serum, 1% glucose, 15% shrimp meat extract and 5% lactalbumin hydrolysate. The optimum conditions for primary culture in vitro were obtained in 2 × L-15 medium with an osmolality of 770 mmol/kg at temperature range of 25-28°C. The optimum pH was 7.4. Both epithelial-like and fibroblastic-like cells were observed from lymphoid organ within 2 days incubation. Within three days, 80% confluent monolayers were obtained from lymphoid organ while cultures from other tissues required five days. Cultures were maintained for up to at least 43 days. Only cells from lymphoid organ could be subcultured and confluent monolayers achieved within 10 days post-split. Healthy cultures of the lymphoid cells did not persist beyond the third passage. These primary shrimp cell cultures supported growth of shrimp viruses like WSSV and YHV, and cytopathic effects of these two viruses were characterized.
Kasornchandra, J., & Khongpradit, R. (2005). Development of shrimp (Penaeus monodon) cell culture in vitro. In K. Nagasawa (Ed.), Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia (pp. 59–66). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
Showing items related by title, author, creator and subject.
Development of monoclonal antibody (MAb) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) Catap, Elena S.; de la Peña, Leobert D. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)Antibody-based detection methods are relatively simple and rapid, aside from being specific and sensitive. Thus, there is a necessity to develop such techniques for early and rapid diagnosis of viral pathogens such as MBV ...
Level and percentage recovery of resistance to oxytetracycline and oxolinic acid of bacteria from shrimp ponds The bacterial level of the water, sediment and cultured shrimp (Penaeus monodon) from different ponds were determined using a general medium, a presumptive Vibrio medium and a presumptive Pseudomonas–Aeromonas medium. ...
Prevalence of yellow head virus in cultured black tiger shrimp (Penaeus monodon Fabricius) from selected shrimp farms in the Philippines Natividad, Karlo Dante T.; Magbanua, Fe O.; Migo, Veronica P.; Alfafara, Catalino G.; Albaladejo, Juan D.; Nadala, Jr., Elpidio Cesar B.; Loh, Philip C.; Tapay, Lourdes M. (Fish Health Section, Asian Fisheries Society, 2002)The prevalence and geographic distribution of Yellow head virus (YHV) in cultured Penaeus monodon in the Philippines was documented by Western blot assay. Fifty-three out of 219 samples (24.2%) were found to be positive ...