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dc.contributor.authorde la Peña, Leobert D.
dc.contributor.authorCruz-Lacierda, Erlinda R.
dc.contributor.authorBaguisi, Kathrine Marie S.
dc.contributor.authorNapa, Jane Frances
dc.contributor.authorSombito, Christopher D.
dc.contributor.authorTendencia, Eleonor
dc.contributor.authorPaner, Milagros G.
dc.contributor.editorNagasawa, Kazuya
dc.date.accessioned2020-07-22T00:45:25Z
dc.date.available2020-07-22T00:45:25Z
dc.date.issued2005-03
dc.identifier.citationde la Peña, L. D., Cruz-Lacierda, E. R., Baguisi, K. M. S., Napa, J. F., Sombito, C. D., Tendencia, E. A., & Paner, M. G. (2005). Establishment of preventive measures against viral nervous necrosis (VNN) in finfish hatcheries. In K. Nagasawa (Ed.), Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia (pp. 115–125). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.en
dc.identifier.isbn9718511732
dc.identifier.urihttp://hdl.handle.net/10862/5919
dc.description.abstractThe establishment of preventive measures against viral nervous necrosis (VNN) at the finfish hatcheries of SEAFDEC Aquaculture Department in Iloilo, Philippines, was instituted primarily to minimize the impact of the viral infection in cultured stocks. Selection of VNN-negative broodstocks was conducted and the detection rate ranged from 25-56%. It was confirmed that all species of broodstocks are already contaminated with the virus with varying percentages. The combination of cell culture and RT-PCR in the detection of the virus proved to be more sensitive than the RT-PCR alone. The trash fish food was suspected as a main source of contamination and tests prove that most of the trash fish species were contaminated with the virus. The effect of chemical treatment was tested and a good result was obtained by using 5 and 7 ppm iodine. Several recommendations were formulated in the prevention and control of VNN in the hatchery phase: VNN-negative broodstocks should be selected by using cell culture and PCR; spawning of the selected broodstocks should be limited to only six times and these fertilized eggs should be used for larval productions; fertilized eggs should be washed and disinfected with iodine; and commercial broodstock feeds should be used instead of trash fish.en
dc.language.isoenen
dc.publisherAquaculture Department, Southeast Asian Fisheries Development Centeren
dc.subjectdisease preventionen
dc.subjectEpinephelus coioidesen
dc.subjectLates calcariferen
dc.subjectLutjanus argentimaculatusen
dc.subjectChanos chanosen
dc.titleEstablishment of preventive measures against viral nervous necrosis (VNN) in finfish hatcheriesen
dc.typeBook chapteren
dc.citation.spage115
dc.citation.epage125
dc.citation.bookTitleRecent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asiaen
dc.subject.asfahatcheriesen
dc.subject.asfanecrosisen
dc.subject.asfamaricultureen
dc.subject.asfafish cultureen
dc.subject.asfafish diseasesen
dc.subject.asfahistopathologyen
dc.subject.asfabreeding stocken
dc.subject.asfavirusesen
dc.subject.asfanucleic acidsen
dc.subject.asfafish eggsen
dc.subject.asfadisinfectionen
dc.subject.asfatrash fishen
dc.subject.asfacell cultureen
dc.subject.scientificNameChanos chanos
dc.subject.scientificNameEpinephelus fuscoguttatusen
dc.subject.scientificNameLates calcariferen


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