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dc.contributor.authorLudevese-Pascual, Gladys
dc.contributor.authorAhmed, Farhana
dc.contributor.authorDe Troch, Marleen
dc.contributor.authorAmar, Edgar
dc.contributor.authorLaranja, Joseph Leopoldo
dc.contributor.authorBode, Samuel
dc.contributor.authorBoeckx, Pascal
dc.contributor.authorBossier, Peter
dc.contributor.authorDe Schryver, Peter
dc.date.accessioned2020-07-22T02:38:40Z
dc.date.available2020-07-22T02:38:40Z
dc.date.issued2020-05-11
dc.identifier.citationLudevese‐Pascual, G., Ahmed, F., De Troch, M., Amar, E., Laranja, J. L., Bode, S., Boeckx, P., Bossier, P., & De Schryver, P. (2020). Determination of poly‐β‐hydroxybutyrate assimilation by postlarval whiteleg shrimp, Litopenaeus vannamei using stable 13C isotope tracing. Journal of the World Aquaculture Society, jwas.12700. https://doi.org/10.1111/jwas.12700en
dc.identifier.issn0893-8849
dc.identifier.urihttp://hdl.handle.net/10862/5929
dc.description.abstractA preliminary study was conducted to demonstrate fate of the bacterial storage compound poly-β-hydroxybutyrate (PHB) once ingested and degraded in vivo in crustaceans. The 2% supplementation of 13C-labeled Ralstonia eutropha DSM545 containing 75% PHB on dry weight in postlarval whiteleg shrimp, Litopenaeus vannamei resulted in consistent enrichment of two fatty acids (containing 14–24 carbons [C] in length) pentadecanoic acid (15:0) and palmitic acid (16:0) in all lipid fractions. This could indicate that 13C signal may not only originate from the PHB but partly from the structural components of the bacterial cell. There was a trend of lower 13C enrichment in various lipid fractions of the postlarvae as compared to the diet. These findings may have important implications as to potential of PHB as energy source rather than as building block. There was a rapid transfer of 13C in the postlarvae as seen by the significant 13C enrichment in the tissues 4 hr after feeding with labeled bacterial cells. Overall, the assimilation of PHB in crustacean tissues is demonstrated for the first time. Our results indicate that PHB once ingested is rapidly assimilated in the tissues and could probably act as an energy source rather than as a building block.en
dc.language.isoenen
dc.publisherWileyen
dc.subjectAssimilationen
dc.subjectLitopenaeus vannameien
dc.titleDetermination of poly‐β‐hydroxybutyrate assimilation by postlarval whiteleg shrimp, Litopenaeus vannamei using stable 13C isotope tracingen
dc.title.alternativeDetermination of poly‐beta‐hydroxybutyrate assimilation by postlarval whiteleg shrimp, Litopenaeus vannamei using stable 13C isotope tracingen
dc.typeArticleen
dc.identifier.doi10.1111/jwas.12700
dc.citation.journalTitleJournal of the World Aquaculture Societyen
dc.subject.asfaFatty acidsen
dc.subject.asfaCarbon isotopesen
dc.subject.asfaIsotopesen
dc.subject.asfaShrimp cultureen
dc.subject.asfaPathogensen
dc.subject.asfaRecirculating systemsen
dc.subject.asfaLipidsen
dc.subject.asfaIsotope fractionationen
dc.subject.asfaStatistical analysisen
dc.subject.asfaPhotoperiodsen
dc.subject.asfaCellsen
dc.subject.asfaFeed compositionen
dc.identifier.essn1749-7345


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