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    In vitro investigation of the anti-koi herpesvirus (KHV) effect of different disinfectants

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    TendenciaEA.pdf (207.4Kb) AQD Access AQD Access
    Downloads: 2
    Date
    2005-03
    Author
    Tendencia, Eleonor ORCID
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    Abstract
    One cause of the rapid spread of koi herpesvirus (KHV) is the trade of live ornamental fish. One way of preventing the spread of the disease is through proper hygiene. This study was done to investigate the virucidal effects of some disinfectants on KHV in vitro. Results of the experiment have shown that KHV (TCID50 = 107.5) exposed to different concentrations of iodine (3, 6, 9 %), benzalkonium chloride (3, 6, 9, 12, 15 %), formalin (0.1, 0.5, 1, 1.5, 2.0%) and hydrogen peroxide (3, 6, 9, 12, 15 %) did not produce cytopathic effect (CPE) on KF-1 cell lines after 7 days incubation at 20°C. Syncitia was observed in cell lines inoculated with KHV exposed to 12 and 15% iodine for 30 min.
    URI
    http://hdl.handle.net/10862/5946
    Suggested Citation
    Tendencia, E. A. (2005). In vitro investigation of the anti-koi herpesvirus (KHV) effect of different disinfectants. In K. Nagasawa (Ed.), Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia (pp. 389–393). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
    Type
    Book chapter
    ISBN
    9718511732
    Subject
    Disinfection; Iodine; Disinfectants; Ornamental fish; Cell culture; Mortality; Fish diseases; Electron microscopy; Polypeptides; Disinfectants; Tissue culture; Benzalkonium chloride; Hydrogen peroxide; Cyprinus carpio; Formalin; Anorexia
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    • Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia [43]


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