Humoral immune response of channel catfish to Flavobacterium columnare antigens, lipopolysaccharide and formalin-killed cells, and the protective ability of these antigens
View/
Date
2002Page views
717ASFA keyword
AGROVOC keyword
Taxonomic term
Metadata
Show full item recordShare
Abstract
The objective of the experiment was to determine the immunogenicity of various concentrations of formalin-killed whole cells of Flavobacterium columnare and lipopolysaccharide (LPS) from the bacterium, with and without Freund s adjuvant. The immunological protective capability of each antigen preparation was then evaluated. Five antigen preparations of F. columnare; the LPS, LPS plus Freund s complete adjuvant (FCA), formalin-killed whole cells (FKWC) at 1 x 107CFU/ml or optical density (OD) of 0.35 at550 nm, 0.35 OD550 FKWC plus FCA and 6 x 1010 CFU/ml or 0.9 OD550 FKWC, were immunogenic when injected intraperitoneally in 2nd year channel catfish (Ictalurus punctatus). An increase in antigen concentration from 1 x 107CFU/ml to 6 x 1010 CFU/ml FKWC did not significantly (p>0.05) increase the antibody titer. The antigen FKWC at a concentration of 1 x 107 CFU/ml was also exposed to the fish by the immersion method. An increase in exposure time of immersion in 1 x 107 CFU/ml from 5 to 15 and 30 min resulted in an increase in antibody titers. Higher antibody production was seen in fish held at 27°C and exposed by immersion to antigen twice. Improved protection in channel catfish fingerlings immunized by intraperitoneal injection with LPS+FCA, FKWC (0.35 OD550), FKWC (0.35 OD550) + FCA, and FKWC (0.9 OD550) antigens over challenged saline and formalin-saline control groups was seen. Fish immunized by immersion in FKWC (OD 0.35550) for 15 min and those immunized by multiple exposure immersion and kept at 27°C also showed improved protection compared to controls or fish immunized by four other immersion immunization regimes.
Suggested Citation
Shamsudin, M. N., & Plumb, J. A. (2002). Humoral immune response of channel catfish to Flavobacterium columnare antigens, lipopolysaccharide and formalin-killed cells, and the protective ability of these antigens. In C. R. Lavilla-Pitogo & E. R. Cruz-Lacierda (Eds.), Diseases in Asian aquaculture IV: Proceedings of the Fourth Symposium on Diseases in Asian Aquaculture, 22-26 November 1999, Cebu City, Philippines (pp. 337-348). Fish Health Section, Asian Fisheries Society.
Type
Conference paperISBN
9718020160Collections
Related items
Showing items related by title, author, creator and subject.
-
Dietary onion and ginger enhance growth, hemato-immunological responses, and disease resistance in brown-marbled grouper, Epinephelus fuscoguttatus
; Amar, Edgar C.
; Faisan, Joseph P., Jr. ; Pakingking, Rolando V., Jr. ; (Bioflux, 2012)
A 12-week (September to December 2009) feeding trial was conducted to evaluate theimmunostimulatory effects of different substances administered orally through the diet in the brown-marbled grouper, Epinephelus fuscoguttatus. Five experimental diets containing either onion, ginger, β–glucan, or vitamin C and a control diet (without immunostimulants) were fed to the fish weighing about 44 g for 12 weeks. Onion-fed fish showed significantly increased weight gain, hematocrit, and total Ig compared to the control group; however, leukocyte differential count and ROS production were unaffected. Ginger-fed fish likewise significantly increased total Ig, ROS production and lysozyme activity. However, it did not affect growth and hematocrit value. β-glucan significantly increased growth and total Ig but had no effect on the other parameters. Vitamin C significantly increased hematocrit, total Ig and ROS production but did not increase growth. Upon challenge with a bacterial pathogen Vibrio harveyi, mortality was significantly reduced in the onion, ginger and vitamin C-fed fish but not in the β–glucan-fed fish. This study demonstrated that onion and ginger could positively affect the innate immune responses and protect grouper against Vibrio harveyi infection. -
Induction of immunity and resistance to white spot syndrome virus (WSSV) in shrimp Penaeus monodon (Fabricius) by synthetic oligodeoxynucleotide and bacterial DNA
Amar, Edgar C.; Faisan, Joseph P., Jr. (University of the Philippines Los Baños, 2012)
Shrimps like all invertebrates are believed to lack true adaptive immunity but recent evidence indicate that they can be protected against pathogenic organisms by priming their immune system with immunostimulatory substances. Here, we describe results of investigation aimed to strengthen shrimp immunity as a preventive strategy against white spot syndrome virus (WSSV) infection. Oligodeoxynucleotides (ODNs) with and without Cytosine-Guanine (CpG) motifs, and Vibrio harveyi genomic DNA (VHD) were administered by intramuscular injection and shrimp responses were assessed by ex vivo assays and experimental infection trials. Results showed that CpG ODN significantly increased ex-vivo immunity indices such as total hemocyte count (THC), plasma agglutination titer (PAT) and hemocyte lysate agglutination titer (HLAT). VHD significantly increased immune indices such as THC, plasma total protein (PTP) and HLAT. Reverse (GpC) motifs increased THC only. At a lower viral challenge dose, both CpG and GpC motifs, and VHD, were able to reduce shrimp mortality significantly but only CpG and VHD did so at a higher challenge dose. Strengthening shrimp immunity by the use of immunostimulatory nucleotides and bacterial genomic DNA could be a feasible preventive approach in the management of WSSV infections in shrimp. -
Evaluation of dietary freeze-dried Chaetoceros calcitrans supplementation to control Vibrio harveyi infection on Penaeus monodon juvenile
; ; ; ; Amar, Edgar (Elsevier, 2014)
Effects of supplementation of diets with freeze-dried Chaetoceros calcitrans to control Vibrio harveyi infection are evaluated through immune responses, and disease resistance of juvenile Penaeus monodon. Total lipid and fatty acid profile of Chaetoceros calcitrans is also analyzed. A challenge infection with 107 cfu/mL concentration of Vibrio harveyi is intramuscularly injected to juvenile Penaeus monodon after 45 days of feeding of diets supplemented with 15 g/kg and 30 g/kg dried Chaetoceros calcitrans. The use of dried Chaetoceros calcitrans is compared with that of ß-1,3 glucan Curdlan, a commercial immune enhancer. Incorporation of 30 g/kg Chaetoceros calcitrans in the diet enhances the immune system of shrimp as effected by high prophenoloxidase activity and plasma protein concentration and is better compared to the commercially available Curdlan. Chaetoceros calcitrans also contains polyunsaturated fatty acids (PUFAs) such as linolenic acid and eicosapentanoic acid (EPA) which are responsible for its antibacterial action against Vibrio harveyi. All these biological activities of Chaetoceros calcitrans add up to increase resistance of the juvenile Penaeus monodon to vibriosis as shown by its high survival rate from the challenge infection with Vibrio harveyi. Therefore, it is worthwhile to use Chaetoceros calcitrans as supplementary feed. Its effect in increasing the immune competence coupled with its antibacterial action, make the shrimp resistant to luminous vibriosis that continues to affect the industry, thereby augmenting aquaculture production.
