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dc.contributor.authorShamsudin, Mariana Nor
dc.contributor.authorPlumb, John A.
dc.contributor.editorLavilla-Pitogo, Celia R.
dc.contributor.editorCruz-Lacierda, Erlinda R.
dc.date.accessioned2021-11-18T00:48:02Z
dc.date.available2021-11-18T00:48:02Z
dc.date.issued2002
dc.identifier.citationShamsudin, M. N., & Plumb, J. A. (2002). Humoral immune response of channel catfish to Flavobacterium columnare antigens, lipopolysaccharide and formalin-killed cells, and the protective ability of these antigens. In C. R. Lavilla-Pitogo & E. R. Cruz-Lacierda (Eds.), Diseases in Asian aquaculture IV: Proceedings of the Fourth Symposium on Diseases in Asian Aquaculture, 22-26 November 1999, Cebu City, Philippines (pp. 337-348). Fish Health Section, Asian Fisheries Society.en
dc.identifier.isbn9718020160
dc.identifier.urihttp://hdl.handle.net/10862/6225
dc.description.abstractThe objective of the experiment was to determine the immunogenicity of various concentrations of formalin-killed whole cells of Flavobacterium columnare and lipopolysaccharide (LPS) from the bacterium, with and without Freund s adjuvant. The immunological protective capability of each antigen preparation was then evaluated. Five antigen preparations of F. columnare; the LPS, LPS plus Freund s complete adjuvant (FCA), formalin-killed whole cells (FKWC) at 1 x 107CFU/ml or optical density (OD) of 0.35 at550 nm, 0.35 OD550 FKWC plus FCA and 6 x 1010 CFU/ml or 0.9 OD550 FKWC, were immunogenic when injected intraperitoneally in 2nd year channel catfish (Ictalurus punctatus). An increase in antigen concentration from 1 x 107CFU/ml to 6 x 1010 CFU/ml FKWC did not significantly (p>0.05) increase the antibody titer. The antigen FKWC at a concentration of 1 x 107 CFU/ml was also exposed to the fish by the immersion method. An increase in exposure time of immersion in 1 x 107 CFU/ml from 5 to 15 and 30 min resulted in an increase in antibody titers. Higher antibody production was seen in fish held at 27°C and exposed by immersion to antigen twice. Improved protection in channel catfish fingerlings immunized by intraperitoneal injection with LPS+FCA, FKWC (0.35 OD550), FKWC (0.35 OD550) + FCA, and FKWC (0.9 OD550) antigens over challenged saline and formalin-saline control groups was seen. Fish immunized by immersion in FKWC (OD 0.35550) for 15 min and those immunized by multiple exposure immersion and kept at 27°C also showed improved protection compared to controls or fish immunized by four other immersion immunization regimes.en
dc.publisherFish Health Section, Asian Fisheries Societyen
dc.subjectimmune responseen
dc.subjectlipopolysaccharidesen
dc.subjectformalinen
dc.subjectIctalurus punctatusen
dc.subjectfreshwater catfishen
dc.subjectagglutination testsen
dc.titleHumoral immune response of channel catfish to Flavobacterium columnare antigens, lipopolysaccharide and formalin-killed cells, and the protective ability of these antigensen
dc.typeConference paperen
dc.citation.spage337en
dc.citation.epage348en
dc.citation.conferenceTitleDiseases in Asian aquaculture IV: Proceedings of the Fourth Symposium on Diseases in Asian Aquaculture, 22-26 November 1999, Cebu City, Philippinesen
dc.subject.asfaimmunityen
dc.subject.asfaantigensen
dc.subject.asfamarine organismsen
dc.subject.asfafish diseasesen
dc.subject.asfaantibodiesen
dc.subject.scientificNameFlavobacterium columnareen


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