Motile aeromonads recovered from tilapia (Oreochromis niloticus) cultured in earthen ponds in the Philippines: Assessment of antibiotic susceptibility and multidrug resistance to selected antibiotics
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Abstract
A total of 177 motile Aeromonas spp., comprised of A. hydrophila (n=162) and A. sobria (n=15), isolated from the water, sediment, gills and intestines of tilapia collected from the grow-out culture earthen ponds in the Philippines were investigated for their antimicrobial susceptibility to 10 antibiotics commonly used in fish aquaculture. All motile Aeromonas spp. tested generally exhibited a very high percentage of resistance to amoxicillin (A. hydrophila: 95%; A. sobria: 100%), erythromycin (99%;100%), neomycin (83%;100%), and oxytetracycline (92%;100%), respectively. On the contrary, they showed substantial susceptibility to chloramphenicol, nitrofurantoin, doxycycline, enrofloxacin and norfloxacin. Results of the antimicrobial susceptibility testing demonstrated that 94.4% and 100% of A. hydrophila and A. sobria isolates tested were resistant to antibiotics belonging to at least 3 or more categories and therefore considered as multiple drug resistant strains. The multiple antibiotic resistance (MAR) indexing of A. hydrophila and A. sobria strains showed that they originated from high-risk sources.
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antimicrobials Aeromonas oxytetracycline amoxicillin MAR index tilapia Oreochromis niloticusSuggested Citation
Pakingking, R. V., Jr., Espanola, J. G., Palma, P., & Usero, R. (2022). Motile aeromonads recovered from tilapia (Oreochromis niloticus) cultured in earthen ponds in the Philippines: Assessment of antibiotic susceptibility and multidrug resistance to selected antibiotics. Israeli Journal of Aquaculture - Bamidgeh , 74, 1-11. https://doi.org/10.46989/001c.37010
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Quantitative and qualitative analyses of the bacterial microbiota of tilapia (Oreochromis niloticus) cultured in earthen ponds in the Philippines
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Aeromonas load and species composition in tilapia (Oreochromis niloticus) cultured in earthen ponds in the Philippines
Pakingking, Rolando V., Jr.; Palma, Peter ; (Wiley, 2020-04-14)
Aeromonas load and species composition in the rearing water, sediment, gills and intestines of healthy tilapia Oreochromis niloticus collected every 2 weeks from Days 30 to 120 after stocking in six earthen ponds in the Philippines were determined. Presumptive Aeromonas counts (PACs) in the water and sediment ranged from 101–103 c.f.u./ml and 101–103 c.f.u./g while in the gills and intestines, PACs ranged from 104–107 c.f.u./g and 102–105 c.f.u./g, respectively. Presumptive Aeromonas counts in the water, sediment, and gills of tilapia varied among days of culture while in the intestines of tilapia, PACs markedly dropped by approximately 2 logs at Day 75 and either remained in the same level or decreased by another 1 log at Day 120 of grow‐out culture. Aeromonas hydrophila predominantly constituted 94% of all presumptive Aeromonas spp. examined (n = 343), followed by A. sobria (4%) and A. salmonicida (2%). Taken together, current data provide some putative threshold levels of tilapia reared in earthen ponds to Aeromonas spp. The dominance of A. hydrophila together with negligible population of A. salmonicida and A. sobria indicate that Aeromonas are common commensal bacteria in tilapia and their environment which under conditions of stress could instigate disease epizootics.
