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dc.contributor.authorBatbatan, Christopher
dc.contributor.authorHepowit, Nathaniel
dc.contributor.authorOclarit, Jose M.
dc.identifier.citationBatbatan, C. G., Hepowit, N. L., & Oclarit, J. M. (2011). Developmental historicity and saccharide heterotrophy of Schizochytrium sp. OT01: Implication of docosahexaenoic acid production for biotechnological applications. Asia Life Sciences, 20(2), 289-305.en
dc.description.abstractThraustochytrids have been targeted worldwide in search of highly oleaginous strains for industrial applications and newly collected holotypes are described based on life cycle developmental history and saccharide heterotrophy. Schizochytrium sp. OT01, a novel thraustochytrid protist from Panay, Philippines, distinguished from other reported strains by its asexual reproductive history - including the successive binary division of vegetative cells forming tetrads or octads, granular maturation of thalli into zoosporangia liberating heterokont motile zoospores and transformation of spheroidal thalli into amoeboid cells. Live-cell differential imaging and electron micrographs support its distinctive morphological features, such as hair-like pseudopodial astrals along hyaline margins of mature limaciform amoeboids and in motile zoospores prior to heterokont flagellum elongation. Moreover, its heterotrophic culture on certain saccharides (glucose, fructose, cellobiose, sucrose and starch) in continuously agitated fermentation condition was further investigated to determine hydrocarbon assimilative profiles as deduced from biomass yield and polyunsaturated fatty acid production. Increasing biomass yield was observed in increasing concentrations of saccharides; however, aside from glucose and fructose, a graphical drop of biomass was apparent beyond 15 gL, implying osmotic stress at these levels. Maximum biomass yield was observed at 30 gL- glucose and fructose concentration, reaching to about 6 g/l as expressed in freeze-dried weight of harvested cells, which indicates that OT01 could efficiently absorb and metabolize glucose and fructose compared to other hydrocarbons with relatively greater molecular weights. Even though biomass yield amounts were relatively low in cellobiose, sucrose and starch; however, docosahexaenoic acid (DHA) content in total fatty acids was relatively higher, amounting to 45.23, 46.25 and 48.86%, respectively. Moreover, oleagineity levels in glucose and fructose were highest, with lipid yield amounting to 655.55 and 609.38 mg, respectively, in every gram of freeze-dried weight cells. Consequently, DHA yield was significantly higher in both glucose and fructose, reaching respectively to 492.04 and 478.18 mg per liter of nutrient broth, which indicates higher absorption and metabolism efficiency of monosaccharides into fatty acids.en
dc.description.sponsorshipWith collaborative efforts of the Mindanao State University - Iligan Institute of Technology, this research was supported by the Southeast Asian Fisheries Development Center (Aquaculture Department) under Project Code FD-03-Y2005T through the Biotech Fund of the National Fisheries Research and Development Institute (Bureau of Fisheries and Aquatic Resources).en
dc.publisherRushing Water Publishersen
dc.titleDevelopmental historicity and saccharide heterotrophy of Schizochytrium sp. OT01: Implication of docosahexaenoic acid production for biotechnological applicationsen
dc.citation.journalTitleAsia Life Sciencesen
dc.subject.asfapolyunsaturated fatty acidsen
dc.subject.asfalife cycleen
dc.subject.asfafatty acidsen
dc.subject.asfadevelopmental stagesen
local.subjectdocosahexaenoic aciden
local.subjectsaccharide heterotrophyen
local.subjectSchizochytrium sp. OT01en
local.subjectmetabolism efficiencyen
local.subjectpolyunsaturated fatty acidsen

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    These papers were contributed by Department staff to various national and international journals.

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