In vitro and in vivo evaluation of the efficacies of commercial probiotics and disinfectant against acute hepatopancreatic necrosis disease and luminescent vibriosis in Litopenaeus vannamei

Abstract

The bioactivities of two commercially available probiotics and one chemical disinfectant were tested against strains of <em>Vibrio parahaemolyticus</em> (VP_AHPND) and <em>V. harveyi</em>. This study aimed to determine shrimp pathogenic Vibrios' <em>in vitro</em> and <em>in vivo</em> sensitivities to commercial probiotics and a chemical disinfectant. The probiotics and disinfectant were tested first <em>in vitro,</em> followed by the <em>in vivo</em> trials. Results showed that upon administration of probiotics either through diet or adding into the tank water, the survivability of shrimp was increased during challenge with VP_AHPND and <em>V. harveyi</em>. Also, the disinfectant was tested against the same pathogens and showed positive bactericidal effects at 2500 ppm and 5000 ppm. The present findings suggest that adding probiotics to the rearing water or the shrimp feeds effectively prevents infection by lowering the load of pathogenic bacteria. In comparison, the effectiveness of the disinfectant (PUR) depends on its appropriate concentration and timing of application. It is not only limited to rearing water but is also applicable for decontaminating pond liners, tanks, and other paraphernalia.