Immunological and molecular biology techniques in disease diagnosis
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The improvement of existing immunoassay techniques, development of monoclonal antibody technology and the development of new immunoassay approaches are all working together to provide new tools for the detection of disease-causing organisms in fish and crustaceans. Following the introduction of nucleic acid hybridization technique and PCR, it was recognized that the methods offered a sensitive approach to the detection and identification of specific microorganisms as in the case of a bacterial or viral infection in a variety of sample types. Potentially, a characteristic DNA sequence from a single virus particle or cell of a particular organism can be amplified to detectable levels within a short period of time. Conventional diagnostic methods that involve the culture of microorganisms can take days or weeks to complete or very tedious to perform. PCR offers a rapid, very sensitive, very specific and simple alternative. Further developments in immunodiagnostics and emerging technologies such as DNA-based tests will revolutionize the detection and identification of infectious disease agents.
de la Peña, L. D. (2001). Immunological and molecular biology techniques in disease diagnosis. In G. D. Lio-Po, C. R. Lavilla, & E. R. Cruz-Lacierda (Eds.), Health Management in Aquaculture (pp. 137-158). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
PublisherAquaculture Department, Southeast Asian Fisheries Development Center
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Book chapterGD Lio-Po & LHS Lim - In PTK Woo, DW Bruno & LHS Lim (Eds.), Diseases and disorders of finfish in cage culture, 2014 - CABI PublishingThis chapter presents the viral, bacterial, pseudofungal and parasitic diseases in cultured warm freshwater fish. Focus is given on the distribution, causative agent, pathology, diagnosis, prevention and control of these diseases.
BookA textbook on diseases of cultured warmwater fish and shrimps in the Philippines. Eleven chapters cover essential information on the basic principles of disease causation, major diseases of cultured fish and crustaceans, particularly shrimps, and methods of prevention and control. Emphasis is made on major diseases that occur in the Philippines and other countries in the Asian region. Included also are topics on harmful algae, immunology and molecular biological diagnostic techniques.
BookCR Lavilla-Pitogo, GD Lio-Po, ER Cruz-Lacierda, EV Alapide-Tendencia & LD de la Peña - 2000 - Aquaculture Department, Southeast Asian Fisheries Development Center
Series: Aquaculture extension manual / SEAFDEC. Aquaculture Department; No. 16The manual provides information on the diseases that affect the 3 major species of shrimps cultured in the Philippines: Penaeus monodon, P. merguiensis and P. indicus. It includes the common name of the disease, causative agent, species affected, stages affected, gross signs, effects on the host and methods of prevention and treatment. This revised edition includes newly discovered diseases. It is hoped that the manual will be of considerable help to shrimp farmers in identifying the disease and lead to prevention or early disease diagnosis and control.