Localisation of enzymes in the digestive system during early development of the grouper (Epinephelus coioides)
Abstract
This study was undertaken to investigate the occurrence of some digestive enzymes in the gastrointestinal tract during early development in the grouper. This work was conducted to provide information on formulating an appropriate feeding scheme and an artificial diet for the early development of the grouper, Epinephelus coioides. Larvae of E. coioides were reared in 5 tonne rectangular concrete tanks. The digestive enzymes localized were acid phosphatase (ACP), alkaline phosphatase (ALP), nonspecific esterase (NSE), aminopeptidase (AMP), trypsin (TRP), maltase (MAL) and lipase (LIP). Weak enzyme activity occurred during the yolk sac stage. High AMP activity started at day 14 prior to Artemia feeding at day 16. Fluctuations in TRP activity might be related to stomach formation. Occurrence of MAL during early development demonstrated a capacity to digest carbohydrates. An increase in LIP activity coincided with the occurrence of gastric glands. Insignificant changes in digestive enzymes were observed in the metamorphosing grouper larvae from day 40 to 60.
Citation
Quinitio, G. F., Sa-an, A. C., Toledo, J. D., & Tan-Fermin, J. D. (2004). Localisation of enzymes in the digestive system during early development of the grouper (Epinephelus coioides). In M. A. Rimmer, S. McBride, & K. C. Williams (Eds.), Advances in grouper aquaculture (pp. 30–34). Canberra: Australian Centre for International Agricultural Research.
Publisher
Australian Centre for International Agricultural ResearchSeries
ACIAR Monograph 110Subject
Type
Book chapterISBN
9781863204385Collections
- Book Chapters [82]
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Showing items related by title, author, creator and subject.
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Article
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Article
Apparent digestibility coefficient of nutrients from shrimp, mussel, diatom and seaweed by juvenile Holothuria scabra Jaeger
-Aquaculture Research , 2014 - WileyThe ability of Holothuria scabra to digest nutrients, such as organic matter (OM), protein and carbohydrate from animal and plant feed ingredients was investigated. Four test feeds prepared by mixing sand with single ingredients from animal sources (shrimp and mussel) and plant sources (diatom and seaweed) were fed to H. scabra to estimate apparent digestibility coefficient (ADC). The total assimilated nutrient (TAN) increased with ADC, whereas ingestion rate (IR) varied slightly among the feeds suggesting that ADC might be a good indicator of nutrient availability to H. scabra. The ADCOM of shrimp and mussel was significantly higher than that diatom and seaweed: 86.2%, 77.1%, 55.1% and 32.3% respectively. ADCprotein was similar for shrimp (88.7%), mussel (84.8%) and diatom (75.2%), but significantly lower in seaweed (34.4%). ADCcarbohydrate was similar in mussel (58.5%) and diatom (58.3%) as well as in seaweed (31.6) and shrimp (28.0%). ADCprotein was relatively higher than ADCcarbohydrate suggesting that H. scabra generally digests more protein than carbohydrate. Furthermore, results indicated that nutrients from animal-based feeds are more efficiently digested by H. scabra; thus, animal ingredients rich in easily digestible protein could potentially provide an efficiently balanced diet for H. scabra fed with diatom containing high easily digestible carbohydrate.
