Targeting essential gene utilizing RNA interference to protect the ailing shrimp/prawn industry against WSSV
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The white spot syndrome virus (WSSV) remains to be the most widespread and devastating infectious agent that has hit particularly the marine shrimp aquaculture industry worldwide. To date, there are no known effective strategies that can combat WSSV infection. This study aimed to elucidate host-pathogen interaction through the functional study of host - gene. Utilizing RNA Interference, the function of contig23 (c23) in the shrimp genome, identified to have high homology with WSSVORF-325, was determined. Three set-ups were prepared for treatment of c23-, GFP-dsRNA, and PBS using Macrobrachium rosenbergii freshwater prawns. Each treatment group was challenged with WSSV and survival rate was recorded. C23-, and GFP-dsRNA injected prawns showed a significant survival rate of 100%, in contrast to 20% of the PBS injected prawns at 10 days post-infection (dpi). Results showed that injection of c23- and GFP-dsRNA prior to challenge with WSSV, delayed and reduced mortality in contrast to PBS-treated prawns, which showed high mortality. Gene expression analysis showed silencing of both WSSV and c23 at day 3 post-WSSV challenge. This study proved that c23-dsRNA has a protective effect on WSSVchallenged prawns and highlights its involvement in the infectivity of WSSV in M. rosenbergii.
Lazarte, J. M. S., &Maningas, M. B. B. (2015). Targeting essential gene utilizing RNA interference to protect the ailing shrimp/prawn industry against WSSV. In M. R. R. Romana-Eguia, F. D. Parado-Estepa, N. D. Salayo, &M. J. H. Lebata-Ramos (Eds.), Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia: Challenges in Responsible Production of Aquatic Species: Proceedings of the International Workshop on Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia 2014 (RESA) (pp. 283-298). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center. http://hdl.handle.net/10862/2783
PublisherAquaculture Department, Southeast Asian Fisheries Development Center
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BookCR Lavilla-Pitogo, GD Lio-Po, ER Cruz-Lacierda, EV Alapide-Tendencia & LD de la Peña - 2000 - Aquaculture Department, Southeast Asian Fisheries Development Center
Series: Aquaculture extension manual; No. 16The manual provides information on the diseases that affect the 3 major species of shrimps cultured in the Philippines: Penaeus monodon, P. merguiensis and P. indicus. It includes the common name of the disease, causative agent, species affected, stages affected, gross signs, effects on the host and methods of prevention and treatment. This revised edition includes newly discovered diseases. It is hoped that the manual will be of considerable help to shrimp farmers in identifying the disease and lead to prevention or early disease diagnosis and control.
Conference paperY Wong & J Jiang - In RV Pakingking Jr., EGT de Jesus-Ayson & BO Acosta (Eds.), Addressing Acute Hepatopancreatic Necrosis Disease (AHPND) and Other Transboundary Diseases for Improved Aquatic … Diseases for Improved Aquatic Animal Health in Southeast Asia, 22-24 February 2016, Makati City, Philippines, 2016 - Aquaculture Department, Southeast Asian Fisheries Development CenterSingapore has a small shrimp farming industry with approximately 54 metric tons (MT) of shrimps produced in one year from both land and coastal farms. There is also one shrimp farm producing broodstock for export. Singapore has the capability to diagnose acute hepatopancreatic necrosis disease (AHPND) through histopathology and the isolation of its causal agent which is Vibrio parahaemolyticus. The polymerase chain reaction (PCR) method for AHPND detection in shrimp is currently being undertaken to further strengthen its laboratory and diagnostic capacity. Notably, Singapore is still AHPND-free. On the contrary, white spot disease caused by white spot syndrome virus (WSSV) is a disease of concern as it affects the trade for ornamental crustaceans. Singapore has an active surveillance program for WSSV and other transboundary pathogens of penaeid shrimps. Positive detections would be followed by movement controls and stamping out protocols.
Current status of acute hepatopancreatic necrosis disease (AHPND) and other transboundary diseases of farmed shrimps in Indonesia MS Hastuti & Desrina - In RV Pakingking Jr., EGT de Jesus-Ayson & BO Acosta (Eds.), Addressing Acute Hepatopancreatic Necrosis Disease (AHPND) and Other Transboundary Diseases for Improved Aquatic … Diseases for Improved Aquatic Animal Health in Southeast Asia, 22-24 February 2016, Makati City, Philippines, 2016 - Aquaculture Department, Southeast Asian Fisheries Development CenterTransboundary diseases have been a constant challenge for the aquaculture industry in Indonesia. In spite of this, Indonesian aquaculture has experienced a steady growth since 2010. Early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) is a serious emerging transboundary disease of cultured shrimp that has not been reported in Indonesia. On the contrary, hepatopancreatic microsporidiosis (HPM) was first detected in 2015. Other previously reported transboundary diseases of shrimp and fish include white spot disease (WSD), Taura syndrome and infectious myonecrosis (IMN), and viral nervous necrosis (VNN) and koi herpesvirus (KHV), respectively. These diseases have been included in the surveillance program conducted in 2016. To avert the spread of these transboundary pathogens in the Indonesian aquaculture facilities and natural waters, competent authorities have been tasked to implement stringent control measures including government policy and regulation, active and passive surveillance, and strengthening farmers and stake holders awareness of the importance of disease control and health maintenance.