Targeting essential gene utilizing RNA interference to protect the ailing shrimp/prawn industry against WSSV
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The white spot syndrome virus (WSSV) remains to be the most widespread and devastating infectious agent that has hit particularly the marine shrimp aquaculture industry worldwide. To date, there are no known effective strategies that can combat WSSV infection. This study aimed to elucidate host-pathogen interaction through the functional study of host - gene. Utilizing RNA Interference, the function of contig23 (c23) in the shrimp genome, identified to have high homology with WSSVORF-325, was determined. Three set-ups were prepared for treatment of c23-, GFP-dsRNA, and PBS using Macrobrachium rosenbergii freshwater prawns. Each treatment group was challenged with WSSV and survival rate was recorded. C23-, and GFP-dsRNA injected prawns showed a significant survival rate of 100%, in contrast to 20% of the PBS injected prawns at 10 days post-infection (dpi). Results showed that injection of c23- and GFP-dsRNA prior to challenge with WSSV, delayed and reduced mortality in contrast to PBS-treated prawns, which showed high mortality. Gene expression analysis showed silencing of both WSSV and c23 at day 3 post-WSSV challenge. This study proved that c23-dsRNA has a protective effect on WSSVchallenged prawns and highlights its involvement in the infectivity of WSSV in M. rosenbergii.
Lazarte, J. M. S., &Maningas, M. B. B. (2015). Targeting essential gene utilizing RNA interference to protect the ailing shrimp/prawn industry against WSSV. In M. R. R. Romana-Eguia, F. D. Parado-Estepa, N. D. Salayo, &M. J. H. Lebata-Ramos (Eds.), Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia: Challenges in Responsible Production of Aquatic Species: Proceedings of the International Workshop on Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia 2014 (RESA) (pp. 283-298). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center. http://hdl.handle.net/10862/2783
PublisherAquaculture Department, Southeast Asian Fisheries Development Center
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BookCR Lavilla-Pitogo, GD Lio-Po, ER Cruz-Lacierda, EV Alapide-Tendencia & LD de la Peña - 2000 - Aquaculture Department, Southeast Asian Fisheries Development Center
Series: Aquaculture extension manual; No. 16The manual provides information on the diseases that affect the 3 major species of shrimps cultured in the Philippines: Penaeus monodon, P. merguiensis and P. indicus. It includes the common name of the disease, causative agent, species affected, stages affected, gross signs, effects on the host and methods of prevention and treatment. This revised edition includes newly discovered diseases. It is hoped that the manual will be of considerable help to shrimp farmers in identifying the disease and lead to prevention or early disease diagnosis and control.
Mortalities of pond-cultured juvenile shrimp, Penaeus monodon, associated with dominance of luminescent vibrios in the rearing environment Severe mortalities due to luminescent vibrios occurred in pond-cultured Penaeus monodon juveniles particularly in the first 45 days of culture. Luminescent vibriosis epizootics led to reduced shrimp production due to mortalities and slow growth of affected stocks. Monitoring of bacterial population in the rearing water of several ponds was conducted from the time the ponds were flooded with water until 60 days of culture to understand the course of infection. Results showed that the occurrence of mortalities was preceded by a shift of the bacterial profile of the rearing water in infected ponds, notably the dominance of luminescent vibrios. Comparison of bacterial load in the rearing water and water source (river or open sea) showed elevated luminescent Vibrio counts in the former at 12 days to 3 weeks after initial entry of water. Histopathology of affected shrimps showed the hepatopancreas as the target organ of infection where severe inflammatory responses in the intertubular sinuses were seen.
Conference paperMG Bondad-Reantaso - In RV Pakingking Jr., EGT de Jesus-Ayson & BO Acosta (Eds.), Addressing Acute Hepatopancreatic Necrosis Disease (AHPND) and Other Transboundary Diseases for Improved Aquatic … Diseases for Improved Aquatic Animal Health in Southeast Asia, 22-24 February 2016, Makati City, Philippines, 2016 - Aquaculture Department, Southeast Asian Fisheries Development CenterThe Organization for Economic Cooperation and Development (OECD) and the Food and Agriculture Organization (FAO) of the United Nations Agricultural Outlook 2015-2024 reported that fisheries production worldwide is projected to expand by 19% between the 2012-14 base period and 2024, to reach 191 million metric tons (MT) and the main driver of this increase will be aquaculture, which is expected to reach 96 million MT by 2024, 38% higher than the base period (average 2012-14) level. Among the 7 key uncertainties that affect gains in productivity, the potential of animal disease outbreaks to affect aquaculture production and subsequently domestic and international markets are once again highlighted, although for the first time in this outlook. Another milestone document, the Blue frontiers: managing the environmental costs of aquaculture identified a number of fish health issues, including increased risk of the spread of pathogens and diseases with intensification, through increased movement of aquatic animals, inter-regional trade and introduction of new species and new strains, and through the use of trash fish or live feed; concerns on residues and development of drug resistant pathogens brought about by the abuse on the use antimicrobials and other veterinary drugs; limited availability of vaccines; environmental stressors that compromise the immune system; difficulties faced by developing countries in implementing international standards; and the need for legislation, enforcement and capacity building. The issues identified then and now are almost the same. Addressing animal health issues in aquaculture is very challenging because the sector is highly complex (with a wide range of diversity in terms of species, systems, practices and environment, each presenting different risks), its fluid environment, and the transboundary nature where fish is considered as one of the most traded commodity, aquatic animals require more attention in order to monitor their health: they are not visible except in tank holding conditions; they live in a complex and dynamic environment and feed consumption and mortalities are hidden under water. This paper looks at the status of a newly emerging disease of cultured shrimp, acute hepatopancreatic necrosis disease (AHPND), which has been recognized as the most important non-viral disease threat to cultured shrimp. In particular, this paper presents the highlights of the International Technical Seminar/Workshop: EMS/AHPND: Government, Scientist and Farmer Responses held from 22-24 June 2015 in Panama City, Panama, which was organized under the auspices of an FAO inter-regional project TCP/INT/3502: Reducing and Managing the Risks of AHPND of Cultured Shrimp, being participated by 11 countries, namely: Colombia, Ecuador, Guatemala, Honduras, Mexico, Panama and Peru from Latin America and the Caribbean (LAC) region and India, Iran, the Philippines and Sri Lanka from the Asian region. The Panama EMS/AHPND June 2015 event aimed to provide a platform to improve the understanding of the disease through the lens of governments, scientists and producers and collectively generate practical management and control measures. More than 100 stakeholders from 21 countries representing the government, academe and producer sectors participated in the event. The highlights contain the latest available information at that time (June 2015) about AHPND including the current state of knowledge about the causative agent, the host and geographical distribution, detection methods, risk factors, management and actions of regional and international organizations.