Evaluation of free essential amino acid in muscle of Nile tilapia (Oreochromis niloticus), as a basis of amino acid requirement for growth.
Share
Abstract
The free essential amino acid levels in muscle of young Nile tilapia (Oreochromis niloticus) after feeding graded amounts of a corresponding test amino acid were measured in 9 separate experiments to determine if free amino acids indicate requirements for growth. Data were analyzed by the broken line regression method. Breakpoints for free threonine and free isoleucine were near the threonine and isoleucine requirements for growth. Breakpoints for free lysine, free histidine, and free valine were much higher than the lysine, histidine, and valine requirements for growth, respectively. Levels of other free essential amino acids in muscle behaved erratically or increased linearly as the dietary test amino acid increased. Amino acid requirements as estimated by levels of free essential amino acids in muscle did not consistently confirm amino acid requirements for growth of Nile tilapia.
Type
Conference paperCollections
- Conference Proceedings [300]
Related items
Showing items related by title, author, creator and subject.
-
Pichia kudriavzevii as feed additive in Nile tilapia (Oreochromis niloticus) diet
; Aya, Frolan
; Watanabe, Kenshi ; ; Aki, Tsunehiro ; Dedeles, Gina (Oxford University Press; Applied Microbiology International, 2024-06)
Yeasts are unicellular eukaryotic microorganisms extensively employed in various applications, notably as an alternative source of protein in feeds, owing to their nutritional benefits. Despite their potential, marine and mangrove yeast species used in the aquaculture industry have received little attention in the Philippines. Pichia kudriavzevii (A2B R1 ISO 3), sourced from bark samples, was selected and mass-produced due to its high protein content and amino acid profile. The dried biomass of P. kudriavzevii was incorporated into the diets of Nile tilapia (Oreochromis niloticus) juveniles at varying inclusion levels (0, 1, 2, and 4 g/kg diet) and its effect on their growth performance, body composition, and liver and intestinal morphology was assessed after 40 days of feeding. The groups that received P. kudriavzevii at a concentration of 2 g/kg diet exhibited higher final body weight, percent weight gain, and specific growth rate in comparison to the other treatment groups. Whole body proximate composition did not vary among the dietary groups. Intestinal and liver histopathology also indicated no abnormalities. These findings suggest the potential of ascomycetous P. kudriavzevii as a beneficial feed additive in Nile tilapia diets, warranting further investigation into its long-term effects and broader applications in fish culture. -
Amino acid requirements for growth of Nile tilapia
(American Society for Nutrition, 1988)A series of feeding experiments was conducted in aquaria to determine the quantitative requirements of the 10 essential amino acids for growth of young Nile tilapia (Oreochromis niloticus). The test diets contained casein and gelatin supplemented by crystalline L-amino acids to provide an amino acid profile similar to 28% whole egg protein except for the test amino acid. Each set of test diets consisted of seven isonitrogenous diets containing varying levels of the amino acid to be tested. Weight gains analyzed by the broken line regression method indicated the following requirements as a percentage of the dietary protein: lysine, 5.12; arginine, 4.20; histidine, 1.72; valine, 2.80; leucine, 3.39; isoleucine, 3.11; threonine, 3.75; tryptophan, 1.00; methionine with cystine (0.54% of the protein), 3.21; and phenylalanine with tyrosine (1.79% of the protein), 5.54. -
The essential nutrients: Proteins and amino acids
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2002)This section aims to teach the reader the ten essential amino acids required by fish and their chemical structures, distinguish between essential and non-essential amino acids; the fate of absorbed amino acids in fish; effects of deficiencies and excesses of dietary amino acids in fish diets; the procedure on how to determine the qualitative and quantitative amino acid requirements of fish; methods of evaluating protein quality; and how to determine protein requirements of some aquaculture species.
