Evaluation of free essential amino acid in muscle of Nile tilapia (Oreochromis niloticus), as a basis of amino acid requirement for growth.
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นามธรรม
The free essential amino acid levels in muscle of young Nile tilapia (Oreochromis niloticus) after feeding graded amounts of a corresponding test amino acid were measured in 9 separate experiments to determine if free amino acids indicate requirements for growth. Data were analyzed by the broken line regression method. Breakpoints for free threonine and free isoleucine were near the threonine and isoleucine requirements for growth. Breakpoints for free lysine, free histidine, and free valine were much higher than the lysine, histidine, and valine requirements for growth, respectively. Levels of other free essential amino acids in muscle behaved erratically or increased linearly as the dietary test amino acid increased. Amino acid requirements as estimated by levels of free essential amino acids in muscle did not consistently confirm amino acid requirements for growth of Nile tilapia.
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Conference paperคอลเลกชัน
- Conference Proceedings [300]
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Amino acid requirements for growth of Nile tilapia
(American Society for Nutrition, 1988)A series of feeding experiments was conducted in aquaria to determine the quantitative requirements of the 10 essential amino acids for growth of young Nile tilapia (Oreochromis niloticus). The test diets contained casein and gelatin supplemented by crystalline L-amino acids to provide an amino acid profile similar to 28% whole egg protein except for the test amino acid. Each set of test diets consisted of seven isonitrogenous diets containing varying levels of the amino acid to be tested. Weight gains analyzed by the broken line regression method indicated the following requirements as a percentage of the dietary protein: lysine, 5.12; arginine, 4.20; histidine, 1.72; valine, 2.80; leucine, 3.39; isoleucine, 3.11; threonine, 3.75; tryptophan, 1.00; methionine with cystine (0.54% of the protein), 3.21; and phenylalanine with tyrosine (1.79% of the protein), 5.54. -
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